publications scientifiques
publications scientifiques
| 1. | Aubry, M; Kama, M; Henderson, A; Teissier, A; Vanhomwegen, J; Mariteragi-Helle, T; Paoaafaite, T; Manuguerra, J C; Christi, K; Watson, C; Lau, C; Kucharski, A; Cao-Lormeau, V M: Low chikungunya virus seroprevalence two years after emergence in Fiji.. Dans: Int J Infect Dis, 90 , p. 223–25, 2020. (type: Article de journal | Résumé | BibTeX) @article{aubry_low_2020, title = {Low chikungunya virus seroprevalence two years after emergence in Fiji.}, author = {M Aubry and M Kama and A Henderson and A Teissier and J Vanhomwegen and T Mariteragi-Helle and T Paoaafaite and J C Manuguerra and K Christi and C Watson and C Lau and A Kucharski and V M Cao-Lormeau}, year = {2020}, date = {2020-01-01}, journal = {Int J Infect Dis}, volume = {90}, pages = {223--25}, abstract = {OBJECTIVES: In Fiji, autochthonous chikungunya virus (CHIKV) infection was first detected in March 2015. In a previous serosurvey conducted during October-November 2015, we reported a prevalence of anti-CHIKV IgG antibodies of 0.9%. In the present study, we investigated the seroprevalence of CHIKV two years after its emergence in Fiji. METHODS: Sera from 320 residents of Fiji recruited in June 2017, from the same cohort of individuals that participated in the serosurvey in 2015, were tested for the presence of IgG antibodies against CHIKV using a recombinant antigen-based microsphere immunoassay. RESULTS: Between 2015 and 2017, CHIKV seroprevalence among residents increased from 0.9% (3/333) to 12.8% (41/320). Of the participants with available serum samples collected in both 2015 and 2017 (n = 200), 31 (15.5%) who were seronegative in 2015 had seroconverted to CHIKV in 2017. CONCLUSIONS: Our findings suggest that low-level transmission of CHIKV occurred during the two years following the emergence of the virus in Fiji. No CHIKV infection has been reported in Fiji since 2017, but due to the presumed low herd immunity of the population, the risk of CHIKV re-emergence is high. Consequently, chikungunya should be considered in the differential diagnosis of acute febrile diseases in Fiji.}, keywords = {}, pubstate = {published}, tppubtype = {article} } OBJECTIVES: In Fiji, autochthonous chikungunya virus (CHIKV) infection was first detected in March 2015. In a previous serosurvey conducted during October-November 2015, we reported a prevalence of anti-CHIKV IgG antibodies of 0.9%. In the present study, we investigated the seroprevalence of CHIKV two years after its emergence in Fiji. METHODS: Sera from 320 residents of Fiji recruited in June 2017, from the same cohort of individuals that participated in the serosurvey in 2015, were tested for the presence of IgG antibodies against CHIKV using a recombinant antigen-based microsphere immunoassay. RESULTS: Between 2015 and 2017, CHIKV seroprevalence among residents increased from 0.9% (3/333) to 12.8% (41/320). Of the participants with available serum samples collected in both 2015 and 2017 (n = 200), 31 (15.5%) who were seronegative in 2015 had seroconverted to CHIKV in 2017. CONCLUSIONS: Our findings suggest that low-level transmission of CHIKV occurred during the two years following the emergence of the virus in Fiji. No CHIKV infection has been reported in Fiji since 2017, but due to the presumed low herd immunity of the population, the risk of CHIKV re-emergence is high. Consequently, chikungunya should be considered in the differential diagnosis of acute febrile diseases in Fiji. |
| 2. | Henderson, A; Aubry, M; Kama, M; Vanhomwegen, J; Teissier, A; Mariteragi-Helle, T; Paoaafaite, T; Teissier, Y; Watson, C; Manuguerra, J C; Edmunds, W; Withworth, J; Lau, C; Cao-Lormeau, V M; Kucharski, A: Zika seroprevalence declines and neutralizing antibodies wane in adults following outbreaks in French Polynesia and Fiji.. Dans: Elife, 9 (e48460), 2020. (type: Article de journal | Résumé | BibTeX) @article{henderson_zika_2020, title = {Zika seroprevalence declines and neutralizing antibodies wane in adults following outbreaks in French Polynesia and Fiji.}, author = {A Henderson and M Aubry and M Kama and J Vanhomwegen and A Teissier and T Mariteragi-Helle and T Paoaafaite and Y Teissier and C Watson and J C Manuguerra and W Edmunds and J Withworth and C Lau and V M Cao-Lormeau and A Kucharski}, year = {2020}, date = {2020-01-01}, journal = {Elife}, volume = {9}, number = {e48460}, abstract = {It has been commonly assumed that Zika virus (ZIKV) infection confers long-term protection against reinfection, preventing ZIKV from re-emerging in previously affected areas for several years. However, the long-term immune response to ZIKV following an outbreak remains poorly documented. We compared results from eight serological surveys before and after known ZIKV outbreaks in French Polynesia and Fiji, including cross-sectional and longitudinal studies. We found evidence of a decline in seroprevalence in both countries over a two-year period following first reported ZIKV transmission. This decline was concentrated in adults, while high seroprevalence persisted in children. In the Fiji cohort, there was also a significant decline in neutralizing antibody titres against ZIKV, but not against dengue viruses that circulated during the same period.}, keywords = {}, pubstate = {published}, tppubtype = {article} } It has been commonly assumed that Zika virus (ZIKV) infection confers long-term protection against reinfection, preventing ZIKV from re-emerging in previously affected areas for several years. However, the long-term immune response to ZIKV following an outbreak remains poorly documented. We compared results from eight serological surveys before and after known ZIKV outbreaks in French Polynesia and Fiji, including cross-sectional and longitudinal studies. We found evidence of a decline in seroprevalence in both countries over a two-year period following first reported ZIKV transmission. This decline was concentrated in adults, while high seroprevalence persisted in children. In the Fiji cohort, there was also a significant decline in neutralizing antibody titres against ZIKV, but not against dengue viruses that circulated during the same period. |
| 3. | Teissier, Y; Paul, R; Aubry, M; Rodo, X; Dommar, C; Salje, H; Sakuntabhai, A; Cazelles, B; Cao-Lormeau, V M: Long-term persistence of monotypic dengue transmission in small size isolated populations, French Polynesia, 1978-2014.. Dans: Plos Neg Trop Dis, 14 (3), p. e0008110, 2020. (type: Article de journal | Résumé | BibTeX) @article{teissier_long-term_2020, title = {Long-term persistence of monotypic dengue transmission in small size isolated populations, French Polynesia, 1978-2014.}, author = {Y Teissier and R Paul and M Aubry and X Rodo and C Dommar and H Salje and A Sakuntabhai and B Cazelles and V M Cao-Lormeau}, year = {2020}, date = {2020-01-01}, journal = {Plos Neg Trop Dis}, volume = {14}, number = {3}, pages = {e0008110}, abstract = {Understanding the transition of epidemic to endemic dengue transmission remains a challenge in regions where serotypes co-circulate and there is extensive human mobility. French Polynesia, an isolated group of 117 islands of which 72 are inhabited, distributed among five geographically separated subdivisions, has recorded mono-serotype epidemics since 1944, with long inter-epidemic periods of circulation. Laboratory confirmed cases have been recorded since 1978, enabling exploration of dengue epidemiology under monotypic conditions in an isolated, spatially structured geographical location. A database was constructed of confirmed dengue cases, geolocated to island for a 35-year period. Statistical analyses of viral establishment, persistence and fade-out as well as synchrony among subdivisions were performed. Seven monotypic and one heterotypic dengue epidemic occurred, followed by low-level viral circulation with a recrudescent epidemic occurring on one occasion. Incidence was asynchronous among the subdivisions. Complete viral die-out occurred on several occasions with invasion of a new serotype. Competitive serotype replacement has been observed previously and seems to be characteristic of the South Pacific. Island population size had a strong impact on the establishment, persistence and fade-out of dengue cases and endemicity was estimated achievable only at a population size in excess of 175 000. Despite island remoteness and low population size, dengue cases were observed somewhere in French Polynesia almost constantly, in part due to the spatial structuration generating asynchrony among subdivisions. Long-term persistence of dengue virus in this group of island populations may be enabled by island hopping, although could equally be explained by a reservoir of sub-clinical infections on the most populated island, Tahiti.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Understanding the transition of epidemic to endemic dengue transmission remains a challenge in regions where serotypes co-circulate and there is extensive human mobility. French Polynesia, an isolated group of 117 islands of which 72 are inhabited, distributed among five geographically separated subdivisions, has recorded mono-serotype epidemics since 1944, with long inter-epidemic periods of circulation. Laboratory confirmed cases have been recorded since 1978, enabling exploration of dengue epidemiology under monotypic conditions in an isolated, spatially structured geographical location. A database was constructed of confirmed dengue cases, geolocated to island for a 35-year period. Statistical analyses of viral establishment, persistence and fade-out as well as synchrony among subdivisions were performed. Seven monotypic and one heterotypic dengue epidemic occurred, followed by low-level viral circulation with a recrudescent epidemic occurring on one occasion. Incidence was asynchronous among the subdivisions. Complete viral die-out occurred on several occasions with invasion of a new serotype. Competitive serotype replacement has been observed previously and seems to be characteristic of the South Pacific. Island population size had a strong impact on the establishment, persistence and fade-out of dengue cases and endemicity was estimated achievable only at a population size in excess of 175 000. Despite island remoteness and low population size, dengue cases were observed somewhere in French Polynesia almost constantly, in part due to the spatial structuration generating asynchrony among subdivisions. Long-term persistence of dengue virus in this group of island populations may be enabled by island hopping, although could equally be explained by a reservoir of sub-clinical infections on the most populated island, Tahiti. |
| 4. | Togami, E; Gyawali, N; Ong, O; Kama, M; Cao-Lormeau, V M; Aubry, M; Ko, A I; Nilles, E; Collins-Emerson, J M; Devine, G J; Lau, C: First evidence of concurrent enzootic and endemic transmission of Ross River virus in the absence of marsupial reservoirs in Fiji.. Dans: Int J Infect Dis, Epub ahead of print , 2020. (type: Article de journal | Résumé | BibTeX) @article{togami_first_2020, title = {First evidence of concurrent enzootic and endemic transmission of Ross River virus in the absence of marsupial reservoirs in Fiji.}, author = {E Togami and N Gyawali and O Ong and M Kama and V M Cao-Lormeau and M Aubry and A I Ko and E Nilles and J M Collins-Emerson and G J Devine and C Lau}, year = {2020}, date = {2020-01-01}, journal = {Int J Infect Dis}, volume = {Epub ahead of print}, abstract = {BACKGROUND: Ross River virus (RRV) is a zoonotic alphavirus transmitted by several mosquito species. Until recently, endemic transmission was only considered possible in the presence of marsupial reservoirs. METHODS: We investigated RRV seroprevalence in placental mammals, including horses, cows, goats, pigs, dogs, rats, and mice in Fiji, where there are no marsupials. A total of 302 vertebrate serum samples were collected from 86 households from 10 communities in Western Fiji. FINDINGS: Neutralizing antibodies against RRV were detected in 28 to 100% of sera depending on species, and neutralization was strong even at high dilutions. SIGNIFICANCE: Our results are unlikely to be due to cross reactions; Chikungunya is the only other alphavirus known to be present in the Pacific Islands, but it rarely spills over into non-humans, even during epidemics. Our findings, together with recent report of high RRV seroprevalence in humans, strongly suggest that RRV is circulating in Fiji in the absence of marsupial reservoirs. Considering that all non-human vertebrates present in Fiji are panglobal in distribution, RRV has the potential to further expand its geographic range. Further surveillance and access to diagnostics of RRV is critical for the early detection of emergence and outbreaks.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BACKGROUND: Ross River virus (RRV) is a zoonotic alphavirus transmitted by several mosquito species. Until recently, endemic transmission was only considered possible in the presence of marsupial reservoirs. METHODS: We investigated RRV seroprevalence in placental mammals, including horses, cows, goats, pigs, dogs, rats, and mice in Fiji, where there are no marsupials. A total of 302 vertebrate serum samples were collected from 86 households from 10 communities in Western Fiji. FINDINGS: Neutralizing antibodies against RRV were detected in 28 to 100% of sera depending on species, and neutralization was strong even at high dilutions. SIGNIFICANCE: Our results are unlikely to be due to cross reactions; Chikungunya is the only other alphavirus known to be present in the Pacific Islands, but it rarely spills over into non-humans, even during epidemics. Our findings, together with recent report of high RRV seroprevalence in humans, strongly suggest that RRV is circulating in Fiji in the absence of marsupial reservoirs. Considering that all non-human vertebrates present in Fiji are panglobal in distribution, RRV has the potential to further expand its geographic range. Further surveillance and access to diagnostics of RRV is critical for the early detection of emergence and outbreaks. |
| 5. | J., Viallon; Chinain, M; Darius, H T: Revisiting the Neuroblastoma Cell-Based Assay (CBA-N2a) for the Improved Detection of Marine Toxins Active on Voltage Gated Sodium Channels (VGSCs). Dans: Toxins, 12 (5), p. 281, 2020. (type: Article de journal | Résumé | Liens | BibTeX) @article{noauthor_revisiting_2020, title = {Revisiting the Neuroblastoma Cell-Based Assay (CBA-N2a) for the Improved Detection of Marine Toxins Active on Voltage Gated Sodium Channels (VGSCs)}, author = {Viallon J. and M Chinain and H T Darius}, doi = {https://doi.org/10.3390/toxins12050281}, year = {2020}, date = {2020-01-01}, journal = {Toxins}, volume = {12}, number = {5}, pages = {281}, abstract = {The neuroblastoma cell-based assay (CBA-N2a) is widely used for the detection of marine biotoxins in seafood products, yet a consensus protocol is still lacking. In this study, six key parameters of CBA-N2a were revisited: cell seeding densities, cell layer viability after 26 h growth, MTT incubation time, Ouabain and Veratridine treatment and solvent and matrix effects. A step-by-step protocol was defined identifying five viability controls for the validation of CBA-N2a results. Specific detection of two voltage gated sodium channel activators, pacific ciguatoxin (P-CTX3C) and brevetoxin (PbTx3) and two inhibitors, saxitoxin (STX) and decarbamoylsaxitoxin (dc-STX) was achieved, with EC50 values of 1.7 ± 0.35 pg/mL, 5.8 ± 0.9 ng/mL, 3 ± 0.5 ng/mL and 15.8 ± 3 ng/mL, respectively. When applied to the detection of ciguatoxin (CTX)-like toxicity in fish samples, limit of detection (LOD) and limit of quantification (LOQ) values were 0.031 ± 0.008 and 0.064 ± 0.016 ng P-CTX3C eq/g of flesh, respectively. Intra and inter-assays comparisons of viability controls, LOD, LOQ and toxicity in fish samples gave coefficients of variation (CVs) ranging from 3% to 29%. This improved test adaptable to either high throughput screening or composite toxicity estimation is a useful starting point for a standardization of the CBA-N2a in the field of marine toxin detection.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The neuroblastoma cell-based assay (CBA-N2a) is widely used for the detection of marine biotoxins in seafood products, yet a consensus protocol is still lacking. In this study, six key parameters of CBA-N2a were revisited: cell seeding densities, cell layer viability after 26 h growth, MTT incubation time, Ouabain and Veratridine treatment and solvent and matrix effects. A step-by-step protocol was defined identifying five viability controls for the validation of CBA-N2a results. Specific detection of two voltage gated sodium channel activators, pacific ciguatoxin (P-CTX3C) and brevetoxin (PbTx3) and two inhibitors, saxitoxin (STX) and decarbamoylsaxitoxin (dc-STX) was achieved, with EC50 values of 1.7 ± 0.35 pg/mL, 5.8 ± 0.9 ng/mL, 3 ± 0.5 ng/mL and 15.8 ± 3 ng/mL, respectively. When applied to the detection of ciguatoxin (CTX)-like toxicity in fish samples, limit of detection (LOD) and limit of quantification (LOQ) values were 0.031 ± 0.008 and 0.064 ± 0.016 ng P-CTX3C eq/g of flesh, respectively. Intra and inter-assays comparisons of viability controls, LOD, LOQ and toxicity in fish samples gave coefficients of variation (CVs) ranging from 3% to 29%. This improved test adaptable to either high throughput screening or composite toxicity estimation is a useful starting point for a standardization of the CBA-N2a in the field of marine toxin detection. |
| 6. | Chinain, M; Gatti, C M; Roué, M; Darius, H T: Ciguatera-causing dinoflagellates in the genera Gambierdiscus and Fukuyoa: distribution, ecophysiology and toxicology.. Dans: Dinoflagellates: Classification, Evolution, Physiology and Ecological Significance, New-York, 2020. (type: incollection | BibTeX) @incollection{chinain_ciguatera-causing_2020, title = {Ciguatera-causing dinoflagellates in the genera Gambierdiscus and Fukuyoa: distribution, ecophysiology and toxicology.}, author = {M Chinain and C M Gatti and M Roué and H T Darius}, year = {2020}, date = {2020-01-01}, booktitle = {Dinoflagellates: Classification, Evolution, Physiology and Ecological Significance}, address = {New-York}, edition = {Durvasula Subba Rao (Ed.), Nova Science Publishers}, keywords = {}, pubstate = {published}, tppubtype = {incollection} } |
| 7. | Tester, P A; Wicklaffe, L; Jossart, J; Rhodes, L; Enevoldsen, H; Adachi, M; Nishimura, T; Rodriguez, F; Chinain, M; Litaker, R W: Global distribution of the genera Gambierdiscus and Fukuyoa.. Dans: Actes du 18th International Conference on Harmful Algae, 21-26 octobre 2018, Nantes, France, 2020. (type: Inproceedings | BibTeX) @inproceedings{tester_global_2020, title = {Global distribution of the genera Gambierdiscus and Fukuyoa.}, author = {P A Tester and L Wicklaffe and J Jossart and L Rhodes and H Enevoldsen and M Adachi and T Nishimura and F Rodriguez and M Chinain and R W Litaker}, year = {2020}, date = {2020-01-01}, booktitle = {Actes du 18th International Conference on Harmful Algae, 21-26 octobre 2018}, address = {Nantes, France}, keywords = {}, pubstate = {published}, tppubtype = {inproceedings} } |
| 8. | Roué, M; Smith, K F; Sibat, M; Viallon, J; Henry, K; Ung, A; Biessy, L; Hess, P; Darius, H T; Chinain, M: Assessment of Ciguatera and Other Phycotoxin-Related Risks in Anaho Bay (Nuku Hiva Island, French Polynesia): Molecular, Toxicological, and Chemical Analyses of Passive Samplers.. Dans: Toxins, 12 (5), p. 321, 2020. (type: Article de journal | Résumé | BibTeX) @article{roue_assessment_2020, title = {Assessment of Ciguatera and Other Phycotoxin-Related Risks in Anaho Bay (Nuku Hiva Island, French Polynesia): Molecular, Toxicological, and Chemical Analyses of Passive Samplers.}, author = {M Roué and K F Smith and M Sibat and J Viallon and K Henry and A Ung and L Biessy and P Hess and H T Darius and M Chinain}, year = {2020}, date = {2020-01-01}, journal = {Toxins}, volume = {12}, number = {5}, pages = {321}, abstract = {Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed concentrating quantifiable amounts of CTXs on SPATT samplers. The CTX levels varied with increasing deployment time, resin load, and surface area. In addition to CTXs, okadaic acid (OA) and dinophysistoxin-1 (DTX1) were also detected in SPATT extracts using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), consistent with the presence of Gambierdiscus and Prorocentrum species in the environment, as assessed by quantitative polymerase chain reaction (qPCR) and high-throughput sequencing (HTS) metabarcoding analyses conducted on passive window screen (WS) artificial substrate samples. Although these preliminary findings await further confirmation in follow-up studies, they highlight the usefulness of SPATT samplers in the routine surveillance of CP risk on a temporal scale, and the monitoring of other phycotoxin-related risks in ciguatera-prone areas.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed concentrating quantifiable amounts of CTXs on SPATT samplers. The CTX levels varied with increasing deployment time, resin load, and surface area. In addition to CTXs, okadaic acid (OA) and dinophysistoxin-1 (DTX1) were also detected in SPATT extracts using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), consistent with the presence of Gambierdiscus and Prorocentrum species in the environment, as assessed by quantitative polymerase chain reaction (qPCR) and high-throughput sequencing (HTS) metabarcoding analyses conducted on passive window screen (WS) artificial substrate samples. Although these preliminary findings await further confirmation in follow-up studies, they highlight the usefulness of SPATT samplers in the routine surveillance of CP risk on a temporal scale, and the monitoring of other phycotoxin-related risks in ciguatera-prone areas. |
| 9. | Zhen, L; Gatti, C M; Gamarro, Garrido E; Suzuki, A; Teah, H Y: Modeling the time-lag effect of sea surface temperatures on ciguatera poisoning in the South Pacific: Implications for surveillance and response.. Dans: Toxicon, 182 , p. 21–29, 2020. (type: Article de journal | Résumé | BibTeX) @article{zhen_modeling_2020, title = {Modeling the time-lag effect of sea surface temperatures on ciguatera poisoning in the South Pacific: Implications for surveillance and response.}, author = {L Zhen and C M Gatti and E Garrido Gamarro and A Suzuki and H Y Teah}, year = {2020}, date = {2020-01-01}, journal = {Toxicon}, volume = {182}, pages = {21--29}, abstract = {Ciguatera poisoning (CP), arising from ciguatoxins produced by toxic dinoflagellate Gambierdiscus, is one of the most common food-borne diseases in the South Pacific. Climate change as well as its related events have been hypothesized to a higher abundance and wider presence of toxic dinoflagellates, hence a higher risk of the disease. Yet existing studies assessing the relationship between climate factors and CP are limited or based on old data. In this study, we used prewhitened cross-correlation analysis and auto-regressive integrated moving-average (ARIMA) modeling to develop predictive models of monthly CP incidence in Cook Islands and French Polynesia, two ciguatera-endemic regions in the South Pacific, utilizing the latest epidemiological data. Results reveal the significant time-lagged associations between the monthly CP incidence rate and several indicators relating to sea surface temperature (SST). In particular, SST anomaly is proven to be a strong positive predictor of an increased ciguatera incidence for both countries. If these time-lags can be supported by more investigations, it will allow health authorities to take appropriate actions, to limit or avoid an epidemic risk, especially on high-risk climate scenarios.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatera poisoning (CP), arising from ciguatoxins produced by toxic dinoflagellate Gambierdiscus, is one of the most common food-borne diseases in the South Pacific. Climate change as well as its related events have been hypothesized to a higher abundance and wider presence of toxic dinoflagellates, hence a higher risk of the disease. Yet existing studies assessing the relationship between climate factors and CP are limited or based on old data. In this study, we used prewhitened cross-correlation analysis and auto-regressive integrated moving-average (ARIMA) modeling to develop predictive models of monthly CP incidence in Cook Islands and French Polynesia, two ciguatera-endemic regions in the South Pacific, utilizing the latest epidemiological data. Results reveal the significant time-lagged associations between the monthly CP incidence rate and several indicators relating to sea surface temperature (SST). In particular, SST anomaly is proven to be a strong positive predictor of an increased ciguatera incidence for both countries. If these time-lags can be supported by more investigations, it will allow health authorities to take appropriate actions, to limit or avoid an epidemic risk, especially on high-risk climate scenarios. |
| 10. | Dolah, Van F M; Morey, S J; Milne, S; Ung, A; Anderson, P E; Chinain, M: Transcriptomic analysis of polyketide synthases in a highly ciguatoxic dinoflagellate, Gambierdiscus polynesiensis and low toxicity Gambierdiscus pacificus, from French Polynesia. Dans: PLOS One, 15 (4), p. 20231400, 2020. (type: Article de journal | Résumé | Liens | BibTeX) @article{van_dolah_transcriptomic_2020, title = {Transcriptomic analysis of polyketide synthases in a highly ciguatoxic dinoflagellate, Gambierdiscus polynesiensis and low toxicity Gambierdiscus pacificus, from French Polynesia}, author = {F M Van Dolah and S J Morey and S Milne and A Ung and P E Anderson and M Chinain}, doi = {doi:10.1371/journal.pone.0231400}, year = {2020}, date = {2020-01-01}, journal = {PLOS One}, volume = {15}, number = {4}, pages = {20231400}, abstract = {Marine dinoflagellates produce a diversity of polyketide toxins that are accumulated in marine food webs and are responsible for a variety of seafood poisonings. Reef-associated dinoflagellates of the genus Gambierdiscus produce toxins responsible for ciguatera poisoning (CP), which causes over 50,000 cases of illness annually worldwide. The biosynthetic machinery for dinoflagellate polyketides remains poorly understood. Recent transcriptomic and genomic sequencing projects have revealed the presence of Type I modular polyketide synthases in dinoflagellates, as well as a plethora of single domain transcripts with Type I sequence homology. The current transcriptome analysis compares polyketide synthase (PKS) gene transcripts expressed in two species of Gambierdiscus from French Polynesia: a highly toxic ciguatoxin producer, G. polynesiensis, versus a non-ciguatoxic species G. pacificus, each assembled from approximately 180 million Illumina 125 nt reads using Trinity, and compares their PKS content with previously published data from other Gambierdiscus species and more distantly related dinoflagellates. Both modular and single-domain PKS transcripts were present. Single domain β-ketoacyl synthase (KS) transcripts were highly amplified in both species (98 in G. polynesiensis, 99 in G. pacificus), with smaller numbers of standalone acyl transferase (AT), ketoacyl reductase (KR), dehydratase (DH), enoyl reductase (ER), and thioesterase (TE) domains. G. polynesiensis expressed both a larger number of multidomain PKSs, and larger numbers of modules per transcript, than the non-ciguatoxic G. pacificus. The largest PKS transcript in G. polynesiensis encoded a 10,516 aa, 7 module protein, predicted to synthesize part of the polyether backbone. Transcripts and gene models representing portions of this PKS are present in other species, suggesting that its function may be performed in those species by multiple interacting proteins. This study contributes to the building consensus that dinoflagellates utilize a combination of Type I modular and single domain PKS proteins, in an as yet undefined manner, to synthesize polyketides.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Marine dinoflagellates produce a diversity of polyketide toxins that are accumulated in marine food webs and are responsible for a variety of seafood poisonings. Reef-associated dinoflagellates of the genus Gambierdiscus produce toxins responsible for ciguatera poisoning (CP), which causes over 50,000 cases of illness annually worldwide. The biosynthetic machinery for dinoflagellate polyketides remains poorly understood. Recent transcriptomic and genomic sequencing projects have revealed the presence of Type I modular polyketide synthases in dinoflagellates, as well as a plethora of single domain transcripts with Type I sequence homology. The current transcriptome analysis compares polyketide synthase (PKS) gene transcripts expressed in two species of Gambierdiscus from French Polynesia: a highly toxic ciguatoxin producer, G. polynesiensis, versus a non-ciguatoxic species G. pacificus, each assembled from approximately 180 million Illumina 125 nt reads using Trinity, and compares their PKS content with previously published data from other Gambierdiscus species and more distantly related dinoflagellates. Both modular and single-domain PKS transcripts were present. Single domain β-ketoacyl synthase (KS) transcripts were highly amplified in both species (98 in G. polynesiensis, 99 in G. pacificus), with smaller numbers of standalone acyl transferase (AT), ketoacyl reductase (KR), dehydratase (DH), enoyl reductase (ER), and thioesterase (TE) domains. G. polynesiensis expressed both a larger number of multidomain PKSs, and larger numbers of modules per transcript, than the non-ciguatoxic G. pacificus. The largest PKS transcript in G. polynesiensis encoded a 10,516 aa, 7 module protein, predicted to synthesize part of the polyether backbone. Transcripts and gene models representing portions of this PKS are present in other species, suggesting that its function may be performed in those species by multiple interacting proteins. This study contributes to the building consensus that dinoflagellates utilize a combination of Type I modular and single domain PKS proteins, in an as yet undefined manner, to synthesize polyketides. |
| 11. | Bonnard, I; Bornancin, L; Dalle, K; Chinain, M; Zubia, M; Banaigs, B; Roué, M: Assessment of the chemical diversity and potential toxicity of benthic cyanobacterial blooms in the lagoon of Moorea Island (French Polynesia).. Dans: Journal of Marine Science and Engineering, 8 , p. 406, 2020. (type: Article de journal | Résumé | Liens | BibTeX) @article{bonnard_assessment_2020, title = {Assessment of the chemical diversity and potential toxicity of benthic cyanobacterial blooms in the lagoon of Moorea Island (French Polynesia).}, author = {I Bonnard and L Bornancin and K Dalle and M Chinain and M Zubia and B Banaigs and M Roué}, doi = {10.3390/jmse8060406}, year = {2020}, date = {2020-01-01}, journal = {Journal of Marine Science and Engineering}, volume = {8}, pages = {406}, abstract = {In the last decades, an apparent increase in the frequency of benthic cyanobacterial blooms has occurred in coral reefs and tropical lagoons, possibly in part because of global change and anthropogenic activities. In the frame of the survey of marine benthic cyanobacteria proliferating in the lagoon of Moorea Island (French Polynesia), 15 blooms were collected, mainly involving three species—Anabaena sp.1, Lyngbya majuscula and Hydrocoleum majus-B. Their chemical fingerprints, obtained through high performance liquid chromatography combined with UV detection and mass spectrometry (HPLC-UV-MS) analyses, revealed a high extent of species-specificity. The chemical profile of Anabaena sp.1 was characterized by three major cyclic lipopeptides of the laxaphycin family, whereas the one of L. majuscula was characterized by a complex mixture including tiahuramides, trungapeptins and serinol-derived malyngamides. Toxicity screening analyses conducted on these cyanobacterial samples using Artemia salina and mouse neuroblastoma cell-based (CBA-N2a) cytotoxic assays failed to show any toxicity to a degree that would merit risk assessment with regard to public health. However, the apparently increasing presence of blooms of Lyngbya, Hydrocoleum, Anabaena or other benthic cyanobacteria on coral reefs in French Polynesia encourages the implementation of ad hoc monitoring programs for the surveillance of their proliferation and potential assessment of associated hazards.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In the last decades, an apparent increase in the frequency of benthic cyanobacterial blooms has occurred in coral reefs and tropical lagoons, possibly in part because of global change and anthropogenic activities. In the frame of the survey of marine benthic cyanobacteria proliferating in the lagoon of Moorea Island (French Polynesia), 15 blooms were collected, mainly involving three species—Anabaena sp.1, Lyngbya majuscula and Hydrocoleum majus-B. Their chemical fingerprints, obtained through high performance liquid chromatography combined with UV detection and mass spectrometry (HPLC-UV-MS) analyses, revealed a high extent of species-specificity. The chemical profile of Anabaena sp.1 was characterized by three major cyclic lipopeptides of the laxaphycin family, whereas the one of L. majuscula was characterized by a complex mixture including tiahuramides, trungapeptins and serinol-derived malyngamides. Toxicity screening analyses conducted on these cyanobacterial samples using Artemia salina and mouse neuroblastoma cell-based (CBA-N2a) cytotoxic assays failed to show any toxicity to a degree that would merit risk assessment with regard to public health. However, the apparently increasing presence of blooms of Lyngbya, Hydrocoleum, Anabaena or other benthic cyanobacteria on coral reefs in French Polynesia encourages the implementation of ad hoc monitoring programs for the surveillance of their proliferation and potential assessment of associated hazards. |
| 12. | Chinain, M; Gatti, C M; Roué, M; Darius, H T: Ciguatera Poisoning in French Polynesia: insights into the novel trends of an ancient disease.. Dans: New Microbes New Infections, 31 , p. 100565, 2019. (type: Article de journal | BibTeX) @article{5552, title = {Ciguatera Poisoning in French Polynesia: insights into the novel trends of an ancient disease.}, author = {M Chinain and C M Gatti and M Roué and H T Darius}, year = {2019}, date = {2019-01-01}, journal = {New Microbes New Infections}, volume = {31}, pages = {100565}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 13. | Rodier, M; Longo, S; Henry, K; Ung, A; Lo-Yat, A; Darius, H T; Viallon, J; Beker, B; Delesalle, B; Chinain, M: Diversity and toxic potential of algal bloom forming species from Takaroa atoll lagoon (Tuamotu, French Polynesia): a field and mesocosm study.. Dans: Aquatic Microbial Ecology, 83 , p. 15-34, 2019. (type: Article de journal | Résumé | BibTeX) @article{5554, title = {Diversity and toxic potential of algal bloom forming species from Takaroa atoll lagoon (Tuamotu, French Polynesia): a field and mesocosm study.}, author = {M Rodier and S Longo and K Henry and A Ung and A Lo-Yat and H T Darius and J Viallon and B Beker and B Delesalle and M Chinain}, year = {2019}, date = {2019-01-01}, journal = {Aquatic Microbial Ecology}, volume = {83}, pages = {15-34}, abstract = {Pearl farming lagoons are economically important, yet poorly studied ecosystems in French Polynesia. This paper describes a study conducted in 2016 in Takaroa (Tuamotu Archipelago), an atoll recurrently affected by harmful algal bloom (HAB) events. The objectives were to gain insight into phytoplankton community composition, identify the main bloom-forming species and investigate their potential for toxicity. A mesocosm approach was used to assess the response of phytoplankton communities to 3 nutrient treatments: Conway with Si, Si-depleted f/2 and a commercial N-P fertilizer. In total, 87 morpho-species were described from Takaroa lagoon, with dinoflagellates as the most diverse group. Diatoms (Extubocellulus sp., Cylindrotheca closterium, Nitzschia spp.), dinoflagellates (Gymnodinium spp., Heterocapsa spp.) and flagellates (Cryptomonas sp., Pyraminonas spp.) were among the major bloom-forming species identified. Most markedly, Extubocellulus sp., a diatom never reported from French Polynesia before, was able to bloom even in Si-poor environments. Additionally, in vitro cultures of 12 bloom-forming strains were successfully established and tested for their toxicity. Preliminary results suggest that 9 strains, including dinoflagellates (Prorocentrum lima, Amphidinium spp., Heterocapsa sp.), Pyraminonadales (Pyramimonas sp.) and cryptophytes (Cryptomonas sp.), are the likely producers of cyclic imine neurotoxins and toxins acting on voltage-gated sodium channels. The contribution of these toxins to the mortality events previously reported in Takaroa lagoon is further discussed. Overall, this study highlights the relevance of a mesocosm approach which can be applied to other understudied atolls of French Polynesia recurrently threatened by HABs.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pearl farming lagoons are economically important, yet poorly studied ecosystems in French Polynesia. This paper describes a study conducted in 2016 in Takaroa (Tuamotu Archipelago), an atoll recurrently affected by harmful algal bloom (HAB) events. The objectives were to gain insight into phytoplankton community composition, identify the main bloom-forming species and investigate their potential for toxicity. A mesocosm approach was used to assess the response of phytoplankton communities to 3 nutrient treatments: Conway with Si, Si-depleted f/2 and a commercial N-P fertilizer. In total, 87 morpho-species were described from Takaroa lagoon, with dinoflagellates as the most diverse group. Diatoms (Extubocellulus sp., Cylindrotheca closterium, Nitzschia spp.), dinoflagellates (Gymnodinium spp., Heterocapsa spp.) and flagellates (Cryptomonas sp., Pyraminonas spp.) were among the major bloom-forming species identified. Most markedly, Extubocellulus sp., a diatom never reported from French Polynesia before, was able to bloom even in Si-poor environments. Additionally, in vitro cultures of 12 bloom-forming strains were successfully established and tested for their toxicity. Preliminary results suggest that 9 strains, including dinoflagellates (Prorocentrum lima, Amphidinium spp., Heterocapsa sp.), Pyraminonadales (Pyramimonas sp.) and cryptophytes (Cryptomonas sp.), are the likely producers of cyclic imine neurotoxins and toxins acting on voltage-gated sodium channels. The contribution of these toxins to the mortality events previously reported in Takaroa lagoon is further discussed. Overall, this study highlights the relevance of a mesocosm approach which can be applied to other understudied atolls of French Polynesia recurrently threatened by HABs. |
| 14. | Aubry, M; Mapotoeke, M; Teissier, A; Paoaafaite, T; Dumas-Chastang, E; Giard, M; Cao-Lormeau, V M: Dengue virus serotype 2 (DENV-2) outbreak, French Polynesia, 2019.. Dans: Eurosurveillance, 24 , p. 1900407, 2019. (type: Article de journal | Résumé | BibTeX) @article{6411, title = {Dengue virus serotype 2 (DENV-2) outbreak, French Polynesia, 2019.}, author = {M Aubry and M Mapotoeke and A Teissier and T Paoaafaite and E Dumas-Chastang and M Giard and V M Cao-Lormeau}, year = {2019}, date = {2019-01-01}, journal = {Eurosurveillance}, volume = {24}, pages = {1900407}, abstract = {In 1996-97, the last dengue virus serotype 2 (DENV-2) outbreak occurred in French Polynesia. In February 2019, DENV-2 infection was detected in a traveller from New Caledonia. In March, autochthonous DENV-2 infection was diagnosed in two residents. A DENV-2 outbreak was declared on 10 April with 106 cases as at 24 June. Most of the population is not immune to DENV-2; a large epidemic could occur with risk of imported cases in mainland France.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In 1996-97, the last dengue virus serotype 2 (DENV-2) outbreak occurred in French Polynesia. In February 2019, DENV-2 infection was detected in a traveller from New Caledonia. In March, autochthonous DENV-2 infection was diagnosed in two residents. A DENV-2 outbreak was declared on 10 April with 106 cases as at 24 June. Most of the population is not immune to DENV-2; a large epidemic could occur with risk of imported cases in mainland France. |
| 15. | Aubry, M; Cao-Lormeau, V M: History of arthropod-borne virus infections in French Polynesia.. Dans: New Microbes New Infections, 29 , p. 100513, 2019. (type: Article de journal | Résumé | BibTeX) @article{5550, title = {History of arthropod-borne virus infections in French Polynesia.}, author = {M Aubry and V M Cao-Lormeau}, year = {2019}, date = {2019-01-01}, journal = {New Microbes New Infections}, volume = {29}, pages = {100513}, abstract = {In French Polynesia, arthropod-borne diseases are major public health problems. From the mid-1940s, the four serotypes of dengue virus (DENV-1 to -4) have caused 15 epidemics of variable severity. In 2013, for the first time, a sustained co-circulation of two different DENV serotypes (DENV-1 and -3) was reported. The same year, Zika virus (ZIKV) caused the largest outbreak ever recorded at that time. Severe neurologic complications in adults, including Guillain-Barré syndrome and central nervous system malformations in newborns and foeteuses, such as microcephaly, were reported, and a causal link with ZIKV infection was established. In addition to mosquito-borne transmission, the potential for perinatal, sexual and blood-transfusion transmission of ZIKV was demonstrated. In 2014, chikungunya virus (CHIKV) caused an explosive outbreak. Series of Guillain-Barré syndrome temporally associated with the CHIKV epidemic were reported. Except for DENV, ZIKV and CHIKV, no other arboviruses have been detected so far, but serologic evidence suggested the past silent circulation of Ross River virus. From May 2015 DENV-1 has been the only arbovirus transmitted in French Polynesia, but the reemergence of DENV-2 is highly expected since the detection of two autochthonous cases of DENV-2 infection in June 2018.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In French Polynesia, arthropod-borne diseases are major public health problems. From the mid-1940s, the four serotypes of dengue virus (DENV-1 to -4) have caused 15 epidemics of variable severity. In 2013, for the first time, a sustained co-circulation of two different DENV serotypes (DENV-1 and -3) was reported. The same year, Zika virus (ZIKV) caused the largest outbreak ever recorded at that time. Severe neurologic complications in adults, including Guillain-Barré syndrome and central nervous system malformations in newborns and foeteuses, such as microcephaly, were reported, and a causal link with ZIKV infection was established. In addition to mosquito-borne transmission, the potential for perinatal, sexual and blood-transfusion transmission of ZIKV was demonstrated. In 2014, chikungunya virus (CHIKV) caused an explosive outbreak. Series of Guillain-Barré syndrome temporally associated with the CHIKV epidemic were reported. Except for DENV, ZIKV and CHIKV, no other arboviruses have been detected so far, but serologic evidence suggested the past silent circulation of Ross River virus. From May 2015 DENV-1 has been the only arbovirus transmitted in French Polynesia, but the reemergence of DENV-2 is highly expected since the detection of two autochthonous cases of DENV-2 infection in June 2018. |
| 16. | Musso, D; Rovery, C; Loukil, A; Vialette, V; Nguyen, N L: Leprosy in French Polynesia.. Dans: New Microbes New Infections, 29 , p. 100514, 2019. (type: Article de journal | BibTeX) @article{5576, title = {Leprosy in French Polynesia.}, author = {D Musso and C Rovery and A Loukil and V Vialette and N L Nguyen}, year = {2019}, date = {2019-01-01}, journal = {New Microbes New Infections}, volume = {29}, pages = {100514}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 17. | Bouscaren, N; de Coignac, Benoit C; Teissier, Y; Formot, J; Musso, D; Chaix, F; Giard, M: Leptospirosis in French Polynesia: 10 years of surveillance data, 2007-2017.. Dans: New Microbes New Infections, 29 , p. 100518, 2019. (type: Article de journal | Résumé | BibTeX) @article{5551, title = {Leptospirosis in French Polynesia: 10 years of surveillance data, 2007-2017.}, author = {N Bouscaren and C Benoit de Coignac and Y Teissier and J Formot and D Musso and F Chaix and M Giard}, year = {2019}, date = {2019-01-01}, journal = {New Microbes New Infections}, volume = {29}, pages = {100518}, abstract = {Leptospirosis is a worldwide zoonosis with higher incidence in tropical areas and is a neglected disease in the Pacific region. French Polynesia (FP) is a French overseas territory located in the South Pacific. Data on the epidemiology in FP are scarce. In this study, we describe our understanding of leptospirosis epidemiology in FP and discuss the prospects concerning this disease and its surveillance to better address preventive actions. We report 11 years of surveillance data between 1 January 2007 and 31 December 2017. Over the study period, 1356 confirmed and probable leptospirosis cases were reported. The mean annual incidence rate was 46.0 (95% confidence interval, 43.6-48.5) cases per 100?000 inhabitants. We registered 864 (63.7%) hospitalizations; of these, at least 270 (19.9%) were in the intensive care unit, and 24 patients (1.8%) died. Even if the incidence of leptospirosis is lower in FP compared to most of other Pacific countries and territories, our data confirm that the disease is highly endemic in FP. Despite all the preventive measures taken, leptospirosis remains a major public health concern in FP, thus highlighting the need to maintain intensive leptospirosis surveillance, medical staff training and provision of information to the general population.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Leptospirosis is a worldwide zoonosis with higher incidence in tropical areas and is a neglected disease in the Pacific region. French Polynesia (FP) is a French overseas territory located in the South Pacific. Data on the epidemiology in FP are scarce. In this study, we describe our understanding of leptospirosis epidemiology in FP and discuss the prospects concerning this disease and its surveillance to better address preventive actions. We report 11 years of surveillance data between 1 January 2007 and 31 December 2017. Over the study period, 1356 confirmed and probable leptospirosis cases were reported. The mean annual incidence rate was 46.0 (95% confidence interval, 43.6-48.5) cases per 100?000 inhabitants. We registered 864 (63.7%) hospitalizations; of these, at least 270 (19.9%) were in the intensive care unit, and 24 patients (1.8%) died. Even if the incidence of leptospirosis is lower in FP compared to most of other Pacific countries and territories, our data confirm that the disease is highly endemic in FP. Despite all the preventive measures taken, leptospirosis remains a major public health concern in FP, thus highlighting the need to maintain intensive leptospirosis surveillance, medical staff training and provision of information to the general population. |
| 18. | Zidane, M; Ren, Y; Xhaard, C; Leufroy, A; Côte, S; Dewailly, E; Noël, L; Guérin, T; Bouisset, P; Bernagout, S; Paaoafaite, J; Iltis, J; Taquet, M; Suhas, E; Rachédi, F; Boissin, J L; Sebbag, J; Shan, L; Bost-Bezeaud, F; Petitdidier, P: Non-Essential Trace Elements Dietary Exposure in French Polynesia: Intake Assessment, Nail Bio Monitoring and Thyroid Cancer Risk.. Dans: Asian Pac J Cancer Prev, 20 , p. 355-367, 2019. (type: Article de journal | Résumé | BibTeX) @article{5556, title = {Non-Essential Trace Elements Dietary Exposure in French Polynesia: Intake Assessment, Nail Bio Monitoring and Thyroid Cancer Risk.}, author = {M Zidane and Y Ren and C Xhaard and A Leufroy and S Côte and E Dewailly and L Noël and T Guérin and P Bouisset and S Bernagout and J Paaoafaite and J Iltis and M Taquet and E Suhas and F Rachédi and J L Boissin and J Sebbag and L Shan and F Bost-Bezeaud and P Petitdidier}, year = {2019}, date = {2019-01-01}, journal = {Asian Pac J Cancer Prev}, volume = {20}, pages = {355-367}, abstract = {BACKGROUNDIn French Polynesia, thyroid cancer mortality and incidence is reported to be the highest in the world. Excessive levels of non-essential trace elements (nETE) in the body are associated with several types of cancer. OBJECTIVE The present study aims to provide quantitative information on food contamination by mercury (Hg), lead (Pb), arsenic (As) and cadmium (Cd) in French Polynesia and its potential correlation with measurements performed in fingernails of Polynesians, and then to investigate the potential association between these nETE and different thyroid cancer risks. METHODS The study population included 229 interviewed cases and 373 interviewed controls We performed a descriptive analysis of Polynesian food and examined the association between thyroid cancer risk and daily intake levels of nETE and with fingernail nETE levels. RESULTS Hg contamination was mainly present in sea products, Pb contamination was present in almost all samples, Cd was detectable in starchy food and As was detectable in all sea products. No patient exceeded dietary contamination WHO limits for Pb, 2 participants exceeded it for Hg and 3 individuals (0.5%) for cadmium. In fingernail clippings, the most detectable pollutant was Pb (553 participants), then Hg (543 participants) then Cd (only in 130 participants). Thyroid cancer risk was increased more than 4 times by Pb daily intake in patients with a history of cancer in first-degree relatives than in ones without (p for interaction =0.01), and 2 times more in women with more than 3 pregnancies than in those with none or less (p for interaction =0.005); it was also increased following As intake by more than 30% in patients with a history of cancer in first-degree relatives than in ones without (p for interaction =0.05). CONCLUSION Locally produced foods are not a source of nETE exposure in French Polynesia. Dieatry nETE exposure and fingernail nETE concentration are not associated to differentiated thyroid cancer risk. No correlation found between nETE dietary exposure and fingernail nETE concentration.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BACKGROUNDIn French Polynesia, thyroid cancer mortality and incidence is reported to be the highest in the world. Excessive levels of non-essential trace elements (nETE) in the body are associated with several types of cancer. OBJECTIVE The present study aims to provide quantitative information on food contamination by mercury (Hg), lead (Pb), arsenic (As) and cadmium (Cd) in French Polynesia and its potential correlation with measurements performed in fingernails of Polynesians, and then to investigate the potential association between these nETE and different thyroid cancer risks. METHODS The study population included 229 interviewed cases and 373 interviewed controls We performed a descriptive analysis of Polynesian food and examined the association between thyroid cancer risk and daily intake levels of nETE and with fingernail nETE levels. RESULTS Hg contamination was mainly present in sea products, Pb contamination was present in almost all samples, Cd was detectable in starchy food and As was detectable in all sea products. No patient exceeded dietary contamination WHO limits for Pb, 2 participants exceeded it for Hg and 3 individuals (0.5%) for cadmium. In fingernail clippings, the most detectable pollutant was Pb (553 participants), then Hg (543 participants) then Cd (only in 130 participants). Thyroid cancer risk was increased more than 4 times by Pb daily intake in patients with a history of cancer in first-degree relatives than in ones without (p for interaction =0.01), and 2 times more in women with more than 3 pregnancies than in those with none or less (p for interaction =0.005); it was also increased following As intake by more than 30% in patients with a history of cancer in first-degree relatives than in ones without (p for interaction =0.05). CONCLUSION Locally produced foods are not a source of nETE exposure in French Polynesia. Dieatry nETE exposure and fingernail nETE concentration are not associated to differentiated thyroid cancer risk. No correlation found between nETE dietary exposure and fingernail nETE concentration. |
| 19. | Chomerat, N; Bilien, G; Derrien, A; Henry, K; Ung, A; Viallon, J; Darius, H T; Gatti, C M; Roué, M; Hervé, F; Réveillon, D; Amzil, Z; Chinain, M: Ostreopsis lenticularis Y. Fukuyo (Dinophyceae, Gonyaulacales) from French Polynesia (South Pacific Ocean): A revisit of its morphology, molecular phylogeny and toxicity.. Dans: Harmful Algae, 84 , p. 95-111, 2019. (type: Article de journal | Résumé | BibTeX) @article{5553, title = {Ostreopsis lenticularis Y. Fukuyo (Dinophyceae, Gonyaulacales) from French Polynesia (South Pacific Ocean): A revisit of its morphology, molecular phylogeny and toxicity.}, author = {N Chomerat and G Bilien and A Derrien and K Henry and A Ung and J Viallon and H T Darius and C M Gatti and M Roué and F Hervé and D Réveillon and Z Amzil and M Chinain}, year = {2019}, date = {2019-01-01}, journal = {Harmful Algae}, volume = {84}, pages = {95-111}, abstract = {To date, the genus Ostreopsis comprises eleven described species, of which seven are toxigenic and produce various compounds presenting a major threat to human and environmental health. The taxonomy of several of these species however remains controversial, as it was based mostly on morphological descriptions leading, in some cases, to ambiguous interpretations and even possible misidentifications. The species Ostreopsis lenticularis was first described by Y. Fukuyo from French Polynesia using light microscopy observations, but without genetic information associated. The present study aims at revisiting the morphology, molecular phylogeny and toxicity of O. lenticularis based on the analysis of 47 strains isolated from 4 distinct locales of French Polynesia, namely the Society, Australes, Marquesas and Gambier archipelagos. Observations in light, epifluorescence and field emission scanning electron microscopy of several of these strains analyzed revealed morphological features in perfect agreement with the original description of O. lenticularis. Cells were oval, not undulated, 60.594.4?µm in dorso-ventral length, 56.178.2?µm in width, and possessed a typical plate pattern with thecal plates showing two sizes of pores. Phylogenetic analyses inferred from the LSU rDNA and ITS5.8S sequences revealed that the 47 strains correspond to a single genotype, clustering with a strong support with sequences previously ascribed to Ostreopsis sp. 5. Clonal cultures of O. lenticularis were also established and further tested for their toxicity using the neuroblastoma cell-based assay and LCMS/MS analyses. None of the 19 strains tested showed toxic activity on neuroblastoma cells, while LCMS/MS analyses performed on the strains from Tahiti Island (i.e. type locality) confirmed that palytoxin and related structural analogs were below the detection limit. These findings allow to clarify unambiguously the genetic identity of O. lenticularis while confirming previous results from the Western Pacific which indicate that this species shows no toxicity, thus stressing the need to reconsider its current classification within the group of toxic species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To date, the genus Ostreopsis comprises eleven described species, of which seven are toxigenic and produce various compounds presenting a major threat to human and environmental health. The taxonomy of several of these species however remains controversial, as it was based mostly on morphological descriptions leading, in some cases, to ambiguous interpretations and even possible misidentifications. The species Ostreopsis lenticularis was first described by Y. Fukuyo from French Polynesia using light microscopy observations, but without genetic information associated. The present study aims at revisiting the morphology, molecular phylogeny and toxicity of O. lenticularis based on the analysis of 47 strains isolated from 4 distinct locales of French Polynesia, namely the Society, Australes, Marquesas and Gambier archipelagos. Observations in light, epifluorescence and field emission scanning electron microscopy of several of these strains analyzed revealed morphological features in perfect agreement with the original description of O. lenticularis. Cells were oval, not undulated, 60.594.4?µm in dorso-ventral length, 56.178.2?µm in width, and possessed a typical plate pattern with thecal plates showing two sizes of pores. Phylogenetic analyses inferred from the LSU rDNA and ITS5.8S sequences revealed that the 47 strains correspond to a single genotype, clustering with a strong support with sequences previously ascribed to Ostreopsis sp. 5. Clonal cultures of O. lenticularis were also established and further tested for their toxicity using the neuroblastoma cell-based assay and LCMS/MS analyses. None of the 19 strains tested showed toxic activity on neuroblastoma cells, while LCMS/MS analyses performed on the strains from Tahiti Island (i.e. type locality) confirmed that palytoxin and related structural analogs were below the detection limit. These findings allow to clarify unambiguously the genetic identity of O. lenticularis while confirming previous results from the Western Pacific which indicate that this species shows no toxicity, thus stressing the need to reconsider its current classification within the group of toxic species. |
| 20. | Strugarek, M; Bossin, H C; Dumont, Y: On the use of the sterile insect release technique to reduce or eliminate mosquito populations.. Dans: Applied Mathematical Modelling, 68 , p. 443-70, 2019. (type: Article de journal | Résumé | BibTeX) @article{5555, title = {On the use of the sterile insect release technique to reduce or eliminate mosquito populations.}, author = {M Strugarek and H C Bossin and Y Dumont}, year = {2019}, date = {2019-01-01}, journal = {Applied Mathematical Modelling}, volume = {68}, pages = {443-70}, abstract = {Vector control is critical to limit the circulation of vector-borne diseases, like chikungunya, dengue or zika, which have become important issues around the world. Among them, the Sterile Insect Technique (SIT) and the Incompatible Insect Technique (IIT) have recently aroused a renewed interest. In this paper we derive and study a minimalistic mathematical model designed for Aedes mosquito population elimination by SIT/IIT. Contrary to most of the previous models, it is bistable in general, allowing simultaneously for elimination of the population and for its survival. We consider different types of releases (constant, periodic or impulsive) and show necessary conditions to reach elimination in each case. We also estimate both sufficient and minimal treatment times. Biological parameters are estimated from a case study of an Aedes polynesiensis population, for which extensive numerical investigations illustrate the analytical results. The applications of this work are two-fold: to help identifying some key parameters that may need further field investigations, and to help designing release protocols.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Vector control is critical to limit the circulation of vector-borne diseases, like chikungunya, dengue or zika, which have become important issues around the world. Among them, the Sterile Insect Technique (SIT) and the Incompatible Insect Technique (IIT) have recently aroused a renewed interest. In this paper we derive and study a minimalistic mathematical model designed for Aedes mosquito population elimination by SIT/IIT. Contrary to most of the previous models, it is bistable in general, allowing simultaneously for elimination of the population and for its survival. We consider different types of releases (constant, periodic or impulsive) and show necessary conditions to reach elimination in each case. We also estimate both sufficient and minimal treatment times. Biological parameters are estimated from a case study of an Aedes polynesiensis population, for which extensive numerical investigations illustrate the analytical results. The applications of this work are two-fold: to help identifying some key parameters that may need further field investigations, and to help designing release protocols. |
| 21. | Mitchell, P; Mier-y-Teran-Romero, L; Biggerstaff, B; Delorey, M; Aubry, M; Cao-Lormeau, V M; Lozier, M; Cauchemez, S; Johansson, M: Reassessing Serosurvey-Based Estimates of the Zika Symptomatic Proportion.. Dans: Am J Epidemiol, 188 , p. 206-213, 2019. (type: Article de journal | Résumé | BibTeX) @article{5572, title = {Reassessing Serosurvey-Based Estimates of the Zika Symptomatic Proportion.}, author = {P Mitchell and L Mier-y-Teran-Romero and B Biggerstaff and M Delorey and M Aubry and V M Cao-Lormeau and M Lozier and S Cauchemez and M Johansson}, year = {2019}, date = {2019-01-01}, journal = {Am J Epidemiol}, volume = {188}, pages = {206-213}, abstract = { Since the 2007 Zika epidemic in Yap, it has been apparent that not all people infected with Zika virus (ZIKV) experience symptoms. However, the proportion of infections that result in symptoms remains unclear. Existing estimates varied in their interpretation of symptoms due to other causes and the case definition used, and assumed perfect test sensitivity and specificity. Using a Bayesian model and data from ZIKV serosurveys in Yap (2007), French Polynesia (2013-14), and Puerto Rico (2016), we found that assuming perfect sensitivity and specificity generally led to lower estimates of the symptomatic proportion. Incorporating reasonable assumptions for assay sensitivity and specificity, we estimated that 27% (95% Credible Interval: 1537%) (Yap), 44% (26-66%) (French Polynesia), and 50% (34-92%) (Puerto Rico) of infections were symptomatic, with variation due to differences in study populations, study designs, and case definitions. The proportion of ZIKV infections causing symptoms is critical for surveillance system design and impact assessment. Here, we accounted for key uncertainties in existing seroprevalence data and found that estimates for the symptomatic proportion ranged from 27% to 50%, suggesting that while the majority of infections are asymptomatic or mildly symptomatic, symptomatic infections might be more common than previously estimated. },keywords = {}, pubstate = {published}, tppubtype = {article} } <p>Since the 2007 Zika epidemic in Yap, it has been apparent that not all people infected with Zika virus (ZIKV) experience symptoms. However, the proportion of infections that result in symptoms remains unclear. Existing estimates varied in their interpretation of symptoms due to other causes and the case definition used, and assumed perfect test sensitivity and specificity. Using a Bayesian model and data from ZIKV serosurveys in Yap (2007), French Polynesia (2013-14), and Puerto Rico (2016), we found that assuming perfect sensitivity and specificity generally led to lower estimates of the symptomatic proportion. Incorporating reasonable assumptions for assay sensitivity and specificity, we estimated that 27% (95% Credible Interval: 1537%) (Yap), 44% (26-66%) (French Polynesia), and 50% (34-92%) (Puerto Rico) of infections were symptomatic, with variation due to differences in study populations, study designs, and case definitions. The proportion of ZIKV infections causing symptoms is critical for surveillance system design and impact assessment. Here, we accounted for key uncertainties in existing seroprevalence data and found that estimates for the symptomatic proportion ranged from 27% to 50%, suggesting that while the majority of infections are asymptomatic or mildly symptomatic, symptomatic infections might be more common than previously estimated.</p> |
| 22. | Baidaliuk, A; Miot, E; Lequime, S; Moltini-Conclois, I; Delaigue, F; Dabo, S; Dickson, L; Aubry, F; Merkling, S; Cao-Lormeau, V M; Lambrechts, L: Cell-fusing agent virus reduces arbovirus dissemination in Aedes aegypti mosquitoes in vivo.. Dans: J virol, 93 (18), p. e00705-19, 2019. (type: Article de journal | Résumé | BibTeX) @article{6409, title = {Cell-fusing agent virus reduces arbovirus dissemination in Aedes aegypti mosquitoes in vivo.}, author = {A Baidaliuk and E Miot and S Lequime and I Moltini-Conclois and F Delaigue and S Dabo and L Dickson and F Aubry and S Merkling and V M Cao-Lormeau and L Lambrechts}, year = {2019}, date = {2019-01-01}, journal = {J virol}, volume = {93}, number = {18}, pages = {e00705-19}, abstract = { Aedes aegypti mosquitoes are the main vectors of arthropod-borne viruses (arboviruses) of public health significance such as the flaviviruses causing dengue (DENV) and Zika (ZIKV). Mosquitoes are also the natural hosts of a wide range of viruses that are insect-specific, raising the question of their influence on arbovirus transmission in nature. Cell-fusing agent virus (CFAV) was the first described insect-specific flavivirus, initially discovered in an Ae. aegypti cell line and subsequently detected in natural Ae. aegypti populations. It was recently shown that DENV and the CFAV strain isolated from the Ae. aegypti cell line have mutually beneficial interactions in mosquito cells in culture. However, whether natural strains of CFAV and DENV interact in live mosquitoes is unknown. Using a wild-type CFAV isolate recently derived from Thai Ae. aegypti mosquitoes, we found that CFAV negatively interferes with both DENV type 1 and ZIKV, in vitro and in vivo. For both arboviruses, prior infection by CFAV reduced the dissemination titer in mosquito head tissues. Our results indicate that interactions observed between arboviruses and the CFAV strain derived from the cell line might not be a relevant model of the viral interference that we observed in vivo Overall, our study supports the hypothesis that insect-specific flaviviruses may contribute to reduce transmission of human-pathogenic flaviviruses.IMPORTANCE The mosquito Aedes aegypti carries several arthropod-borne viruses (arboviruses) that are pathogenic to humans including dengue and Zika viruses. Interestingly, Ae. aegypti is also naturally infected with insect-only viruses such as cell-fusing agent virus. Although interactions between cell-fusing agent virus and dengue virus have been documented in mosquito cells in culture, whether wild strains of cell-fusing agent virus interfere with arbovirus transmission by live mosquitoes was unknown. We used an experimental approach to demonstrate that cell-fusing agent virus infection reduces the propagation of dengue and Zika viruses in Ae. aegypti These results support the idea that insect-only viruses in nature can modulate the ability of mosquitoes to carry arboviruses of medical significance, and that they could possibly be manipulated to reduce arbovirus transmission. },keywords = {}, pubstate = {published}, tppubtype = {article} } <p>Aedes aegypti mosquitoes are the main vectors of arthropod-borne viruses (arboviruses) of public health significance such as the flaviviruses causing dengue (DENV) and Zika (ZIKV). Mosquitoes are also the natural hosts of a wide range of viruses that are insect-specific, raising the question of their influence on arbovirus transmission in nature. Cell-fusing agent virus (CFAV) was the first described insect-specific flavivirus, initially discovered in an Ae. aegypti cell line and subsequently detected in natural Ae. aegypti populations. It was recently shown that DENV and the CFAV strain isolated from the Ae. aegypti cell line have mutually beneficial interactions in mosquito cells in culture. However, whether natural strains of CFAV and DENV interact in live mosquitoes is unknown. Using a wild-type CFAV isolate recently derived from Thai Ae. aegypti mosquitoes, we found that CFAV negatively interferes with both DENV type 1 and ZIKV, in vitro and in vivo. For both arboviruses, prior infection by CFAV reduced the dissemination titer in mosquito head tissues. Our results indicate that interactions observed between arboviruses and the CFAV strain derived from the cell line might not be a relevant model of the viral interference that we observed in vivo Overall, our study supports the hypothesis that insect-specific flaviviruses may contribute to reduce transmission of human-pathogenic flaviviruses.IMPORTANCE The mosquito Aedes aegypti carries several arthropod-borne viruses (arboviruses) that are pathogenic to humans including dengue and Zika viruses. Interestingly, Ae. aegypti is also naturally infected with insect-only viruses such as cell-fusing agent virus. Although interactions between cell-fusing agent virus and dengue virus have been documented in mosquito cells in culture, whether wild strains of cell-fusing agent virus interfere with arbovirus transmission by live mosquitoes was unknown. We used an experimental approach to demonstrate that cell-fusing agent virus infection reduces the propagation of dengue and Zika viruses in Ae. aegypti These results support the idea that insect-only viruses in nature can modulate the ability of mosquitoes to carry arboviruses of medical significance, and that they could possibly be manipulated to reduce arbovirus transmission.</span></span></p> |
| 23. | Richard, V; Cao-Lormeau, V M: Mosquito vectors of arboviruses in French Polynesia. Dans: New Microbes New Infections, 31 , p. 100569, 2019. (type: Article de journal | BibTeX) @article{6407, title = {Mosquito vectors of arboviruses in French Polynesia}, author = {V Richard and V M Cao-Lormeau}, year = {2019}, date = {2019-01-01}, journal = {New Microbes New Infections}, volume = {31}, pages = {100569}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 24. | Aubry, M; Kama, M; Vanhomwegen, J; Teissier, A; Mariteragi-Helle, T; Hue, S; Hibberd, M L; Manuguerra, J C; Christ, K; Watson, C H; Nilles, E J; Lau, C L; Aaskov, J; Musso, D; Kucharski, A J; Cao-Lormeau, V M: Ross River virus seroprevalence, Fiji, 2013-2015.. Dans: Emerg Infect Dis, 25 , p. 827-30, 2019. (type: Article de journal | Résumé | BibTeX) @article{6410, title = {Ross River virus seroprevalence, Fiji, 2013-2015.}, author = {M Aubry and M Kama and J Vanhomwegen and A Teissier and T Mariteragi-Helle and S Hue and M L Hibberd and J C Manuguerra and K Christ and C H Watson and E J Nilles and C L Lau and J Aaskov and D Musso and A J Kucharski and V M Cao-Lormeau}, year = {2019}, date = {2019-01-01}, journal = {Emerg Infect Dis}, volume = {25}, pages = {827-30}, abstract = { A unique outbreak of Ross River virus (RRV) infection was reported in Fiji in 1979. In 2013, RRV seroprevalence among residents was 46.5% (362/778). Of the residents who were seronegative in 2013 and retested in 2015, 10.9% (21/192) had seroconverted to RRV, suggesting ongoing endemic circulation of RRV in Fiji. },keywords = {}, pubstate = {published}, tppubtype = {article} } <p>A unique outbreak of Ross River virus (RRV) infection was reported in Fiji in 1979. In 2013, RRV seroprevalence among residents was 46.5% (362/778). Of the residents who were seronegative in 2013 and retested in 2015, 10.9% (21/192) had seroconverted to RRV, suggesting ongoing endemic circulation of RRV in Fiji.</p> |
| 25. | Kama, M; Aubry, M; Naivalu, T; Vanhomwegen, J; Mariteragi-Helle, T; Teissier, A; Paoaafaite, T; Hue, S; Hibberd, M L; Manuguerra, J C; Christi, K; Watson, C H; Nilles, E J; Aaskov, J; Lau, C L; Musso, D; Kucharski, A J; Cao-Lormeau, V M: Sustained low-level transmission of Zika and chikungunya viruses following emergence in the Fiji Islands, Pacific.. Dans: Emerg Infect Dis, 25 , p. 1535-8, 2019. (type: Article de journal | Résumé | BibTeX) @article{6408, title = {Sustained low-level transmission of Zika and chikungunya viruses following emergence in the Fiji Islands, Pacific.}, author = {M Kama and M Aubry and T Naivalu and J Vanhomwegen and T Mariteragi-Helle and A Teissier and T Paoaafaite and S Hue and M L Hibberd and J C Manuguerra and K Christi and C H Watson and E J Nilles and J Aaskov and C L Lau and D Musso and A J Kucharski and V M Cao-Lormeau}, year = {2019}, date = {2019-01-01}, journal = {Emerg Infect Dis}, volume = {25}, pages = {1535-8}, abstract = { Zika and chikungunya viruses were first detected in Fiji in 2015. Examining surveillance, phylogenetic, and serologic data, we found evidence of low-level transmission of Zika and chikungunya viruses during 2013–2017, in contrast to the major outbreaks caused by closely related virus strains in other Pacific Island countries. },keywords = {}, pubstate = {published}, tppubtype = {article} } <p>Zika and chikungunya viruses were first detected in Fiji in 2015. Examining surveillance, phylogenetic, and serologic data, we found evidence of low-level transmission of Zika and chikungunya viruses during 2013–2017, in contrast to the major outbreaks caused by closely related virus strains in other Pacific Island countries.</p> |
| 26. | St-Jean, A; Suhas, E; Pina, De J J; Cordier, S; Lucas, M; Ayotte, P: Exposure to toxic metals and polychlorinated biphenyls of adolescentsand adults from two atolls in the Tuamotu Archipelago(French Polynesia). Dans: Science of the total environment, 695 , p. 133791, 2019. (type: Article de journal | Résumé | BibTeX) @article{st-jean_exposure_2019, title = {Exposure to toxic metals and polychlorinated biphenyls of adolescentsand adults from two atolls in the Tuamotu Archipelago(French Polynesia)}, author = {A St-Jean and E Suhas and J J De Pina and S Cordier and M Lucas and P Ayotte}, year = {2019}, date = {2019-01-01}, journal = {Science of the total environment}, volume = {695}, pages = {133791}, abstract = {The atoll of Hao, part of the Tuamotu Archipelago in French Polynesia, hosted an air base which was used by France Air Force and Naval Aviation during the nuclear tests. Following the publication of a report in 2012 indicating widespread contamination of the atoll, we conducted a biomonitoring survey to assess the exposure to toxic metals and polychlorinated biphenyls (PCBs) of Hao residents and residents of Makemo, a nearby atoll without any known sources of industrial pollution. Adults and adolescents (≥12 years) randomly sampled from Hao (n = 275) and Makemo (n = 268) provided blood samples for contaminant analyses. Whole blood samples were analysed for cadmium, lead and total mercury by inductively coupled plasma mass spectrometry. Plasma concentrations of PCBs were measured by gas chromatography mass spectrometry. Face-to-face interviews were conducted to document lifestyle and a food-frequency questionnaire was used to document dietary habits. Concentrations of contaminants were compared between atolls and associations with sociodemographic and personal characteristics of the participants were investigated. A significantly higher mean (geometric) of blood lead concentration was observed in Hao compared to Makemo (3.75 vs 3.40 μg/L, P = 0.02), whereas similar concentrations were noted for cadmium (0.49 vs 0.50 μg/L, P = 0.58) and mercury (11.4 vs 11.5 μg/L, P = 0.78). Mean total PCBs plasma concentration was significantly higher in Hao than in Makemo participants (0.75 vs 0.32 μg/L, P textless 0.001). A significant proportion of participants exceeded toxicological reference values for mercury and lead in both atolls. The higher body burden of PCBs and Pb in Hao compared to Makemo residents may be linked to past air base activities in Hao. According to international standards, PCBs exposure is low; however, exposure to both mercury and lead is high and further investigations are required to identify specific sources of exposure.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The atoll of Hao, part of the Tuamotu Archipelago in French Polynesia, hosted an air base which was used by France Air Force and Naval Aviation during the nuclear tests. Following the publication of a report in 2012 indicating widespread contamination of the atoll, we conducted a biomonitoring survey to assess the exposure to toxic metals and polychlorinated biphenyls (PCBs) of Hao residents and residents of Makemo, a nearby atoll without any known sources of industrial pollution. Adults and adolescents (≥12 years) randomly sampled from Hao (n = 275) and Makemo (n = 268) provided blood samples for contaminant analyses. Whole blood samples were analysed for cadmium, lead and total mercury by inductively coupled plasma mass spectrometry. Plasma concentrations of PCBs were measured by gas chromatography mass spectrometry. Face-to-face interviews were conducted to document lifestyle and a food-frequency questionnaire was used to document dietary habits. Concentrations of contaminants were compared between atolls and associations with sociodemographic and personal characteristics of the participants were investigated. A significantly higher mean (geometric) of blood lead concentration was observed in Hao compared to Makemo (3.75 vs 3.40 μg/L, P = 0.02), whereas similar concentrations were noted for cadmium (0.49 vs 0.50 μg/L, P = 0.58) and mercury (11.4 vs 11.5 μg/L, P = 0.78). Mean total PCBs plasma concentration was significantly higher in Hao than in Makemo participants (0.75 vs 0.32 μg/L, P textless 0.001). A significant proportion of participants exceeded toxicological reference values for mercury and lead in both atolls. The higher body burden of PCBs and Pb in Hao compared to Makemo residents may be linked to past air base activities in Hao. According to international standards, PCBs exposure is low; however, exposure to both mercury and lead is high and further investigations are required to identify specific sources of exposure. |
| 27. | Musso, D; Nhan, T; Pina, De J J; Marchi, J; Texier, G: The Use of Simple Laboratory Parameters in the Differential Diagnosis of Acute-Phase Zika and Dengue Viruses. Dans: Intervirology, 62 (2), p. 51–56, 2019. (type: Article de journal | Résumé | BibTeX) @article{musso_use_2019, title = {The Use of Simple Laboratory Parameters in the Differential Diagnosis of Acute-Phase Zika and Dengue Viruses}, author = {D Musso and T Nhan and J J De Pina and J Marchi and G Texier}, year = {2019}, date = {2019-01-01}, journal = {Intervirology}, volume = {62}, number = {2}, pages = {51--56}, abstract = {Background: Differential diagnosis between acute-phase Zika and dengue is challenging because of a similar clinical presentation and the lack of available molecular diagnosis tools in most of endemic areas. Objectives: Our study aimed to evaluate the use of simple laboratory parameters to differentiate these infections. Methods: We retrospectively compared simple hematology and biochemistry values in 81 and 341 patients with confirmed Zika and dengue, respectively, collected from June 2013 to March 2014 during the French Polynesia outbreaks. Results: Thrombocytopenia, neutropenia, leukopenia, lymphopenia, and elevated aspartate aminotransaminases were significantly more frequent in dengue than in Zika (p textless 0.001). Platelets textless100 × 109/L, neutrophils textless0.5 × 109/L, lymphocytes textless0.5 × 109/L, and aspartate aminotransaminases textgreater100 IU/mL were found in dengue but not in Zika. The positive predictive value of the association of leukocytes textless4 × 109/L + lymphocytes textless1 × 109/L + aspartate aminotransaminases textgreater40 IU/mL for the diagnosis of dengue was 90%, with an accuracy of 82.4%. Conclusion: For the differential diagnosis between acute-phase Zika and dengue, there is no specific standard laboratory pattern. We identified cutoff values and a combination of laboratory parameters that are a strong argument against Zika and in favor of dengue.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Differential diagnosis between acute-phase Zika and dengue is challenging because of a similar clinical presentation and the lack of available molecular diagnosis tools in most of endemic areas. Objectives: Our study aimed to evaluate the use of simple laboratory parameters to differentiate these infections. Methods: We retrospectively compared simple hematology and biochemistry values in 81 and 341 patients with confirmed Zika and dengue, respectively, collected from June 2013 to March 2014 during the French Polynesia outbreaks. Results: Thrombocytopenia, neutropenia, leukopenia, lymphopenia, and elevated aspartate aminotransaminases were significantly more frequent in dengue than in Zika (p textless 0.001). Platelets textless100 × 109/L, neutrophils textless0.5 × 109/L, lymphocytes textless0.5 × 109/L, and aspartate aminotransaminases textgreater100 IU/mL were found in dengue but not in Zika. The positive predictive value of the association of leukocytes textless4 × 109/L + lymphocytes textless1 × 109/L + aspartate aminotransaminases textgreater40 IU/mL for the diagnosis of dengue was 90%, with an accuracy of 82.4%. Conclusion: For the differential diagnosis between acute-phase Zika and dengue, there is no specific standard laboratory pattern. We identified cutoff values and a combination of laboratory parameters that are a strong argument against Zika and in favor of dengue. |
| 28. | St-Jean, A; Suhas, E; Pina, De J J; Cordier, S; Lucas, M; Ayotte, P: Exposure to toxic metals and polychlorinated biphenyls of adolescentsand adults from two atolls in the Tuamotu Archipelago(French Polynesia). Dans: Science of the total environment, 695 , p. 133791, 2019. (type: Article de journal | Résumé | BibTeX) @article{st-jean_exposure_2019b, title = {Exposure to toxic metals and polychlorinated biphenyls of adolescentsand adults from two atolls in the Tuamotu Archipelago(French Polynesia)}, author = {A St-Jean and E Suhas and J J De Pina and S Cordier and M Lucas and P Ayotte}, year = {2019}, date = {2019-01-01}, journal = {Science of the total environment}, volume = {695}, pages = {133791}, abstract = {The atoll of Hao, part of the Tuamotu Archipelago in French Polynesia, hosted an air base which was used by France Air Force and Naval Aviation during the nuclear tests. Following the publication of a report in 2012 indicating widespread contamination of the atoll, we conducted a biomonitoring survey to assess the exposure to toxic metals and polychlorinated biphenyls (PCBs) of Hao residents and residents of Makemo, a nearby atoll without any known sources of industrial pollution. Adults and adolescents (≥12 years) randomly sampled from Hao (n = 275) and Makemo (n = 268) provided blood samples for contaminant analyses. Whole blood samples were analysed for cadmium, lead and total mercury by inductively coupled plasma mass spectrometry. Plasma concentrations of PCBs were measured by gas chromatography mass spectrometry. Face-to-face interviews were conducted to document lifestyle and a food-frequency questionnaire was used to document dietary habits. Concentrations of contaminants were compared between atolls and associations with sociodemographic and personal characteristics of the participants were investigated. A significantly higher mean (geometric) of blood lead concentration was observed in Hao compared to Makemo (3.75 vs 3.40 μg/L, P = 0.02), whereas similar concentrations were noted for cadmium (0.49 vs 0.50 μg/L, P = 0.58) and mercury (11.4 vs 11.5 μg/L, P = 0.78). Mean total PCBs plasma concentration was significantly higher in Hao than in Makemo participants (0.75 vs 0.32 μg/L, P textless 0.001). A significant proportion of participants exceeded toxicological reference values for mercury and lead in both atolls. The higher body burden of PCBs and Pb in Hao compared to Makemo residents may be linked to past air base activities in Hao. According to international standards, PCBs exposure is low; however, exposure to both mercury and lead is high and further investigations are required to identify specific sources of exposure.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The atoll of Hao, part of the Tuamotu Archipelago in French Polynesia, hosted an air base which was used by France Air Force and Naval Aviation during the nuclear tests. Following the publication of a report in 2012 indicating widespread contamination of the atoll, we conducted a biomonitoring survey to assess the exposure to toxic metals and polychlorinated biphenyls (PCBs) of Hao residents and residents of Makemo, a nearby atoll without any known sources of industrial pollution. Adults and adolescents (≥12 years) randomly sampled from Hao (n = 275) and Makemo (n = 268) provided blood samples for contaminant analyses. Whole blood samples were analysed for cadmium, lead and total mercury by inductively coupled plasma mass spectrometry. Plasma concentrations of PCBs were measured by gas chromatography mass spectrometry. Face-to-face interviews were conducted to document lifestyle and a food-frequency questionnaire was used to document dietary habits. Concentrations of contaminants were compared between atolls and associations with sociodemographic and personal characteristics of the participants were investigated. A significantly higher mean (geometric) of blood lead concentration was observed in Hao compared to Makemo (3.75 vs 3.40 μg/L, P = 0.02), whereas similar concentrations were noted for cadmium (0.49 vs 0.50 μg/L, P = 0.58) and mercury (11.4 vs 11.5 μg/L, P = 0.78). Mean total PCBs plasma concentration was significantly higher in Hao than in Makemo participants (0.75 vs 0.32 μg/L, P textless 0.001). A significant proportion of participants exceeded toxicological reference values for mercury and lead in both atolls. The higher body burden of PCBs and Pb in Hao compared to Makemo residents may be linked to past air base activities in Hao. According to international standards, PCBs exposure is low; however, exposure to both mercury and lead is high and further investigations are required to identify specific sources of exposure. |
| 29. | Musso, D; Nhan, T; Pina, De J J; Marchi, J; Texier, G: The Use of Simple Laboratory Parameters in the Differential Diagnosis of Acute-Phase Zika and Dengue Viruses. Dans: Intervirology, 62 (2), p. 51–56, 2019. (type: Article de journal | Résumé | BibTeX) @article{musso_use_2019b, title = {The Use of Simple Laboratory Parameters in the Differential Diagnosis of Acute-Phase Zika and Dengue Viruses}, author = {D Musso and T Nhan and J J De Pina and J Marchi and G Texier}, year = {2019}, date = {2019-01-01}, journal = {Intervirology}, volume = {62}, number = {2}, pages = {51--56}, abstract = {Background: Differential diagnosis between acute-phase Zika and dengue is challenging because of a similar clinical presentation and the lack of available molecular diagnosis tools in most of endemic areas. Objectives: Our study aimed to evaluate the use of simple laboratory parameters to differentiate these infections. Methods: We retrospectively compared simple hematology and biochemistry values in 81 and 341 patients with confirmed Zika and dengue, respectively, collected from June 2013 to March 2014 during the French Polynesia outbreaks. Results: Thrombocytopenia, neutropenia, leukopenia, lymphopenia, and elevated aspartate aminotransaminases were significantly more frequent in dengue than in Zika (p textless 0.001). Platelets textless100 × 109/L, neutrophils textless0.5 × 109/L, lymphocytes textless0.5 × 109/L, and aspartate aminotransaminases textgreater100 IU/mL were found in dengue but not in Zika. The positive predictive value of the association of leukocytes textless4 × 109/L + lymphocytes textless1 × 109/L + aspartate aminotransaminases textgreater40 IU/mL for the diagnosis of dengue was 90%, with an accuracy of 82.4%. Conclusion: For the differential diagnosis between acute-phase Zika and dengue, there is no specific standard laboratory pattern. We identified cutoff values and a combination of laboratory parameters that are a strong argument against Zika and in favor of dengue.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background: Differential diagnosis between acute-phase Zika and dengue is challenging because of a similar clinical presentation and the lack of available molecular diagnosis tools in most of endemic areas. Objectives: Our study aimed to evaluate the use of simple laboratory parameters to differentiate these infections. Methods: We retrospectively compared simple hematology and biochemistry values in 81 and 341 patients with confirmed Zika and dengue, respectively, collected from June 2013 to March 2014 during the French Polynesia outbreaks. Results: Thrombocytopenia, neutropenia, leukopenia, lymphopenia, and elevated aspartate aminotransaminases were significantly more frequent in dengue than in Zika (p textless 0.001). Platelets textless100 × 109/L, neutrophils textless0.5 × 109/L, lymphocytes textless0.5 × 109/L, and aspartate aminotransaminases textgreater100 IU/mL were found in dengue but not in Zika. The positive predictive value of the association of leukocytes textless4 × 109/L + lymphocytes textless1 × 109/L + aspartate aminotransaminases textgreater40 IU/mL for the diagnosis of dengue was 90%, with an accuracy of 82.4%. Conclusion: For the differential diagnosis between acute-phase Zika and dengue, there is no specific standard laboratory pattern. We identified cutoff values and a combination of laboratory parameters that are a strong argument against Zika and in favor of dengue. |
| 30. | Longo, S; Sibat, M; Viallon, J; Darius, H T; Hess, P; Chinain, M: Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia. Dans: Toxins, 11 (12), p. 735, 2019. (type: Article de journal | Résumé | BibTeX) @article{longo_intraspecific_2019, title = {Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia}, author = {S Longo and M Sibat and J Viallon and H T Darius and P Hess and M Chinain}, year = {2019}, date = {2019-01-01}, journal = {Toxins}, volume = {11}, number = {12}, pages = {735}, abstract = {Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds, all strains showed high overall P-CTX production ranging from 1.1 ± 0.1 to 4.6 ± 0.7 pg cell−1. In total, nine P-CTX analogs were detected, depending on strain and growth phase. The production of gambierone, as well as 44-methylgamberione, was also confirmed in G. polynesiensis. This study highlighted: (i) intraspecific variations in toxin production and profiles between clones from distinct geographic origins and (ii) the noticeable increase in toxin production of both CTXs, in particular CTX4A/B, and gambierone group analogs from the exponential to the stationary phase.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds, all strains showed high overall P-CTX production ranging from 1.1 ± 0.1 to 4.6 ± 0.7 pg cell−1. In total, nine P-CTX analogs were detected, depending on strain and growth phase. The production of gambierone, as well as 44-methylgamberione, was also confirmed in G. polynesiensis. This study highlighted: (i) intraspecific variations in toxin production and profiles between clones from distinct geographic origins and (ii) the noticeable increase in toxin production of both CTXs, in particular CTX4A/B, and gambierone group analogs from the exponential to the stationary phase. |
| 31. | Papathanos, P A; Bourtzis, K; Tripet, F; Bossin, H C; Virginio, J F; Capurro, M L; Pedrosa, M C; Guindo, A; Sylla, L; Coulibaly, M B; Yao, F A; Epopa, P S; Diabate, A: A perspective on the need and current status of efficient sex separation methods for mosquito genetic control.. Dans: Parasite Vectors, 11 , p. 654, 2018. (type: Article de journal | Résumé | BibTeX) @article{5805, title = {A perspective on the need and current status of efficient sex separation methods for mosquito genetic control.}, author = {P A Papathanos and K Bourtzis and F Tripet and H C Bossin and J F Virginio and M L Capurro and M C Pedrosa and A Guindo and L Sylla and M B Coulibaly and F A Yao and P S Epopa and A Diabate}, year = {2018}, date = {2018-01-01}, journal = {Parasite Vectors}, volume = {11}, pages = {654}, abstract = {Major efforts are currently underway to develop novel, complementary methods to combat mosquito-borne diseases. Mosquito genetic control strategies (GCSs) have become an increasingly important area of research on account of their species-specificity, track record in targeting agricultural insect pests, and their environmentally non-polluting nature. A number of programs targeting Aedes and Anopheles mosquitoes, vectors of human arboviruses and malaria respectively, are currently being developed or deployed in many parts of the world. Operationally implementing these technologies on a large scale however, beyond proof-of-concept pilot programs, is hampered by the absence of adequate sex separation methods. Sex separation eliminates females in the laboratory from male mosquitoes prior to release. Despite the need for sex separation for the control of mosquitoes, there have been limited efforts in recent years in developing systems that are fit-for-purpose. In this special issue of Parasites and Vectors we report on the progress of the global Coordinated Research Program on “Exploring genetic, molecular, mechanical and behavioural methods for sex separation in mosquitoes” that is led by the Insect Pest Control Subprogramme of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture with the specific aim of building efficient sex separation systems for mosquito species. In an effort to overcome current barriers we briefly highlight what we believe are the three main reasons why progress has been so slow in developing appropriate sex separation systems: the availability of methods that are not scalable, the difficulty of building the ideal genetic systems and, finally, the lack of research efforts in this area.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Major efforts are currently underway to develop novel, complementary methods to combat mosquito-borne diseases. Mosquito genetic control strategies (GCSs) have become an increasingly important area of research on account of their species-specificity, track record in targeting agricultural insect pests, and their environmentally non-polluting nature. A number of programs targeting Aedes and Anopheles mosquitoes, vectors of human arboviruses and malaria respectively, are currently being developed or deployed in many parts of the world. Operationally implementing these technologies on a large scale however, beyond proof-of-concept pilot programs, is hampered by the absence of adequate sex separation methods. Sex separation eliminates females in the laboratory from male mosquitoes prior to release. Despite the need for sex separation for the control of mosquitoes, there have been limited efforts in recent years in developing systems that are fit-for-purpose. In this special issue of Parasites and Vectors we report on the progress of the global Coordinated Research Program on “Exploring genetic, molecular, mechanical and behavioural methods for sex separation in mosquitoes” that is led by the Insect Pest Control Subprogramme of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture with the specific aim of building efficient sex separation systems for mosquito species. In an effort to overcome current barriers we briefly highlight what we believe are the three main reasons why progress has been so slow in developing appropriate sex separation systems: the availability of methods that are not scalable, the difficulty of building the ideal genetic systems and, finally, the lack of research efforts in this area. |
| 32. | Roué, M; Darius, H T; Viallon, J; Ung, A; Gatti, C M; Harwood, D T; Chinain, M: Application of solid phase adsorption toxin tracking (SPATT) devices for the field detection of Gambierdiscus toxins.. Dans: Harmful Algae, 71 , p. 40-9, 2018. (type: Article de journal | Résumé | BibTeX) @article{5583, title = {Application of solid phase adsorption toxin tracking (SPATT) devices for the field detection of Gambierdiscus toxins.}, author = {M Roué and H T Darius and J Viallon and A Ung and C M Gatti and D T Harwood and M Chinain}, year = {2018}, date = {2018-01-01}, journal = {Harmful Algae}, volume = {71}, pages = {40-9}, abstract = {ABSTRACTCiguatera fish poisoning is a food-borne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the Gambierdiscus genus. Since most surveillance programs currently rely on the survey of Gambierdiscus cell densities and species composition, supplementary toxin-based methods allowing the time- and spatially integrated sampling of toxins in ciguateric environments are needed for a more reliable assessment and management of the risks associated with Gambierdiscus proliferation. Solid Phase Adsorption Toxin Tracking (SPATT) filters use porous synthetic resins capable of adsorbing toxins directly from the water column. To assess the ability of these passive monitoring devices to retain Gambierdiscus toxins, SPATT bags filled with 10g of HP20 resin were deployed for 48h in two French Polynesian locations at high (Nuku Hiva Island) vs. low to moderate (Kaukura Atoll) risk of ciguatera. CTXs could be detected in SPATT bags extracts from Nuku Hiva Island, as assessed by the mouse neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography - tandem mass spectrometry (LC-MS/MS) analyses. Results of in vitro experiments suggest that the saturation limit of CTXs on HP20 resin, for a deployment time of 48h, is » 55ng P-CTX-3C equiv. g-1 resin. Despite the non detection of maitotoxin (MTX), LC-MS/MS analyses showed that two other compounds also produced by Gambierdiscus species were retained on SPATT bags, i.e. iso-P-CTX-3B/C and a putative MTX analogue, known as MTX-3. This study, the first to demonstrate the suitability of SPATT technology for the in situ monitoring of Gambierdiscus toxins, highlights the potential application of this tool for routine ciguatera risk assessment and management programs.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ABSTRACTCiguatera fish poisoning is a food-borne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the Gambierdiscus genus. Since most surveillance programs currently rely on the survey of Gambierdiscus cell densities and species composition, supplementary toxin-based methods allowing the time- and spatially integrated sampling of toxins in ciguateric environments are needed for a more reliable assessment and management of the risks associated with Gambierdiscus proliferation. Solid Phase Adsorption Toxin Tracking (SPATT) filters use porous synthetic resins capable of adsorbing toxins directly from the water column. To assess the ability of these passive monitoring devices to retain Gambierdiscus toxins, SPATT bags filled with 10g of HP20 resin were deployed for 48h in two French Polynesian locations at high (Nuku Hiva Island) vs. low to moderate (Kaukura Atoll) risk of ciguatera. CTXs could be detected in SPATT bags extracts from Nuku Hiva Island, as assessed by the mouse neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography - tandem mass spectrometry (LC-MS/MS) analyses. Results of in vitro experiments suggest that the saturation limit of CTXs on HP20 resin, for a deployment time of 48h, is » 55ng P-CTX-3C equiv. g-1 resin. Despite the non detection of maitotoxin (MTX), LC-MS/MS analyses showed that two other compounds also produced by Gambierdiscus species were retained on SPATT bags, i.e. iso-P-CTX-3B/C and a putative MTX analogue, known as MTX-3. This study, the first to demonstrate the suitability of SPATT technology for the in situ monitoring of Gambierdiscus toxins, highlights the potential application of this tool for routine ciguatera risk assessment and management programs. |
| 33. | Bosseboeuf, E; Aubry, M; Nhan, T; Pina, De J J; Rolain, J M; Raoult, D; Musso, D: Azithromycin inhibits the replication of Zika virus.. Dans: J Antivir Antiretrovir, 10 , p. 6-11, 2018. (type: Article de journal | BibTeX) @article{5560, title = {Azithromycin inhibits the replication of Zika virus.}, author = {E Bosseboeuf and M Aubry and T Nhan and J J De Pina and J M Rolain and D Raoult and D Musso}, year = {2018}, date = {2018-01-01}, journal = {J Antivir Antiretrovir}, volume = {10}, pages = {6-11}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 34. | Aubry, M; Laughhunn, A; Maria, Santa F; Lanteri, M C; Stassinopoulos, A; Musso, D: Amustaline (S-303) treatment inactivates high levels of Chikungunya virus in red-blood-cell components.. Dans: Vox Sanguinis, 113 , p. 232-41, 2018. (type: Article de journal | Résumé | BibTeX) @article{5559, title = {Amustaline (S-303) treatment inactivates high levels of Chikungunya virus in red-blood-cell components.}, author = {M Aubry and A Laughhunn and F Santa Maria and M C Lanteri and A Stassinopoulos and D Musso}, year = {2018}, date = {2018-01-01}, journal = {Vox Sanguinis}, volume = {113}, pages = {232-41}, abstract = {Background and objectivesChikungunya virus (CHIKV) infections have been reported in all continents, and the potential risk for CHIKV transfusion-transmitted infections (TTIs) was demonstrated by the detection of CHIKV RNA-positive donations in several countries. TTIs can be reduced by pathogen inactivation (PI) of blood products. In this study, we evaluated the efficacy of amustaline and glutathione (S-303/GSH) to inactivate CHIKV in red-blood-cell concentrates (RBCs). Material and methods Red-blood-cells were spiked with high level of CHIKV. Infectious titres and RNA loads were measured before and after PI treatment. Residual CHIKV infectivity was also assessed after five successive cell culture passages. Results The mean CHIKV titres in RBCs before inactivation was 5·81 ± 0·18 log10 50% tissue culture infectious dose (TCID50 )/mL, and the mean viral RNA load was 10·49 ± 0·15 log10 genome equivalent (GEq)/mL. No CHIKV TCID was detected after S-303 treatment nor was replicative CHIKV particles and viral RNA present after five cell culture passages of samples obtained immediately after S-303 treatment. Conclusion: Chikungunya virus was previously shown to be inactivated by the PI technology using amotosalen and ultraviolet A light for the treatment of plasma and platelets. This new study demonstrates that S-303/GSH can inactivate high titres of CHIKV in RBCs.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background and objectivesChikungunya virus (CHIKV) infections have been reported in all continents, and the potential risk for CHIKV transfusion-transmitted infections (TTIs) was demonstrated by the detection of CHIKV RNA-positive donations in several countries. TTIs can be reduced by pathogen inactivation (PI) of blood products. In this study, we evaluated the efficacy of amustaline and glutathione (S-303/GSH) to inactivate CHIKV in red-blood-cell concentrates (RBCs). Material and methods Red-blood-cells were spiked with high level of CHIKV. Infectious titres and RNA loads were measured before and after PI treatment. Residual CHIKV infectivity was also assessed after five successive cell culture passages. Results The mean CHIKV titres in RBCs before inactivation was 5·81 ± 0·18 log10 50% tissue culture infectious dose (TCID50 )/mL, and the mean viral RNA load was 10·49 ± 0·15 log10 genome equivalent (GEq)/mL. No CHIKV TCID was detected after S-303 treatment nor was replicative CHIKV particles and viral RNA present after five cell culture passages of samples obtained immediately after S-303 treatment. Conclusion: Chikungunya virus was previously shown to be inactivated by the PI technology using amotosalen and ultraviolet A light for the treatment of plasma and platelets. This new study demonstrates that S-303/GSH can inactivate high titres of CHIKV in RBCs. |
| 35. | Martin-Yken, H; Derick, S; Gironde, C; Darius, H T; Furger, C; Laurent, D; Chinain, M: Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: a potential perspective for future biosensors development.. Dans: Environmental Research, 162 , p. 144-51, 2018. (type: Article de journal | Résumé | BibTeX) @article{5569, title = {Ciguatoxins activate the Calcineurin signalling pathway in Yeasts: a potential perspective for future biosensors development.}, author = {H Martin-Yken and S Derick and C Gironde and H T Darius and C Furger and D Laurent and M Chinain}, year = {2018}, date = {2018-01-01}, journal = {Environmental Research}, volume = {162}, pages = {144-51}, abstract = {Ciguatoxins (CTXs) are lipid-soluble polyether compounds produced by dinoflagellates from the genus Gambierdiscus spp. typically found in tropical and subtropical zones. This endemic area is however rapidly expanding due to environmental perturbations, and both toxic Gambierdiscus spp. and ciguatoxic fishes have been recently identified in the North Atlantic Ocean (Madeira and Canary islands) and Mediterranean Sea. Ciguatoxins bind to Voltage Gated Sodium Channels on the membranes of sensory neurons, causing Ciguatera Fish Poisoning (CFP) in humans, a disease characterized by a complex array of gastrointestinal, neurological, neuropsychological, and cardiovascular symptoms. Although CFP is the most frequently reported non bacterial food-borne poisoning worldwide, there is still no simple and quick way of detecting CTXs in contaminated samples. In the prospect to engineer rapid and easy-to-use CTXs live cells-based tests, we have studied the effects of CTXs on the yeast Saccharomyces cerevisiae, a unicellular model which displays a remarkable conservation of cellular signalling pathways with higher eukaryotes. Taking advantage of this high level of conservation, yeast strains have been genetically modified to encode specific transcriptional reporters responding to CTXs exposure. These yeast strains were further exposed to different concentrations of either purified CTX or micro-algal extracts containing CTXs. Our data establish that CTXs are not cytotoxic to yeast cells even at concentrations as high as 1mM, and cause an increase in the level of free intracellular calcium in yeast cells. Concomitantly, a dose-dependent activation of the calcineurin signalling pathway is observed, as assessed by measuring the activity of specific transcriptional reporters in the engineered yeast strains. These findings offer promising prospects regarding the potential development of a yeast cells-based test that could supplement or, in some instances, replace current methods for the routine detection of CTXs in seafood products.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatoxins (CTXs) are lipid-soluble polyether compounds produced by dinoflagellates from the genus Gambierdiscus spp. typically found in tropical and subtropical zones. This endemic area is however rapidly expanding due to environmental perturbations, and both toxic Gambierdiscus spp. and ciguatoxic fishes have been recently identified in the North Atlantic Ocean (Madeira and Canary islands) and Mediterranean Sea. Ciguatoxins bind to Voltage Gated Sodium Channels on the membranes of sensory neurons, causing Ciguatera Fish Poisoning (CFP) in humans, a disease characterized by a complex array of gastrointestinal, neurological, neuropsychological, and cardiovascular symptoms. Although CFP is the most frequently reported non bacterial food-borne poisoning worldwide, there is still no simple and quick way of detecting CTXs in contaminated samples. In the prospect to engineer rapid and easy-to-use CTXs live cells-based tests, we have studied the effects of CTXs on the yeast Saccharomyces cerevisiae, a unicellular model which displays a remarkable conservation of cellular signalling pathways with higher eukaryotes. Taking advantage of this high level of conservation, yeast strains have been genetically modified to encode specific transcriptional reporters responding to CTXs exposure. These yeast strains were further exposed to different concentrations of either purified CTX or micro-algal extracts containing CTXs. Our data establish that CTXs are not cytotoxic to yeast cells even at concentrations as high as 1mM, and cause an increase in the level of free intracellular calcium in yeast cells. Concomitantly, a dose-dependent activation of the calcineurin signalling pathway is observed, as assessed by measuring the activity of specific transcriptional reporters in the engineered yeast strains. These findings offer promising prospects regarding the potential development of a yeast cells-based test that could supplement or, in some instances, replace current methods for the routine detection of CTXs in seafood products. |
| 36. | Angelakis, E; Bachar, D; Yasir, M; Musso, D; Djossou, F; Melenotte, C; Robert, C; Davoust, B; Gaborit, B; Azhar, E I; Bibi, F; Dutour, A; Raoult, D: Comparison of the gut microbiota of obese individuals from different geographic origins.. Dans: New Microbes New Infections, 27 , p. 40-7, 2018. (type: Article de journal | Résumé | BibTeX) @article{5557, title = {Comparison of the gut microbiota of obese individuals from different geographic origins.}, author = {E Angelakis and D Bachar and M Yasir and D Musso and F Djossou and C Melenotte and C Robert and B Davoust and B Gaborit and E I Azhar and F Bibi and A Dutour and D Raoult}, year = {2018}, date = {2018-01-01}, journal = {New Microbes New Infections}, volume = {27}, pages = {40-7}, abstract = {Few studies have examined the interaction of human geography, microbial community structure and obesity. We tested obese adult volunteers from France, Saudi Arabia, French Polynesia and from a traditional population in the village of Trois-Sauts in French Guiana by sequencing the V3-V4 region. We also sequenced homemade fermented cachiri beers that were obtained from the traditional Amazonian population and are highly consumed by this population. We found that French and Saudis had significantly less richness and biodiversity in their gut microbiota than Amazonians and Polynesians (p <0.05). Principle coordinate analysis of the overall composition of the genera communities revealed that the microbiomes of Amazonians clustered independently from the other obese individuals. Moreover, we found that Amazonians presented significantly stricter anaerobic genera than the Saudis, French and Polynesians (p<0.001). Polynesians presented significantly lower relative abundance of Lactobacillus sp. than French (p 0.01) and Saudis (p 0.05). Treponema berlinense and Treponema succinifaciens were only present in the gut microbiome of Amazonians. The cachiri beers presented significantly more bacterial species in common with the gut microbiome of Amazonians (p<0.005). Obese individuals with different origins present modifications in their gut microbiota, and we provide evidence that the cachiri beers influenced the gut microbiome of Amazonians.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Few studies have examined the interaction of human geography, microbial community structure and obesity. We tested obese adult volunteers from France, Saudi Arabia, French Polynesia and from a traditional population in the village of Trois-Sauts in French Guiana by sequencing the V3-V4 region. We also sequenced homemade fermented cachiri beers that were obtained from the traditional Amazonian population and are highly consumed by this population. We found that French and Saudis had significantly less richness and biodiversity in their gut microbiota than Amazonians and Polynesians (p <0.05). Principle coordinate analysis of the overall composition of the genera communities revealed that the microbiomes of Amazonians clustered independently from the other obese individuals. Moreover, we found that Amazonians presented significantly stricter anaerobic genera than the Saudis, French and Polynesians (p<0.001). Polynesians presented significantly lower relative abundance of Lactobacillus sp. than French (p 0.01) and Saudis (p 0.05). Treponema berlinense and Treponema succinifaciens were only present in the gut microbiome of Amazonians. The cachiri beers presented significantly more bacterial species in common with the gut microbiome of Amazonians (p<0.005). Obese individuals with different origins present modifications in their gut microbiota, and we provide evidence that the cachiri beers influenced the gut microbiome of Amazonians. |
| 37. | Sibat, M; Herrenknecht, C; Darius, H T; Roué, M; Chinain, M; Hess, P: Detection of pacific ciguatoxins using liquid chromatography coupled to either low or high resolution mass spectrometry (LC-MS/MS).. Dans: J Chromatogr A, 1571 , p. 16-28, 2018. (type: Article de journal | Résumé | BibTeX) @article{5586, title = {Detection of pacific ciguatoxins using liquid chromatography coupled to either low or high resolution mass spectrometry (LC-MS/MS).}, author = {M Sibat and C Herrenknecht and H T Darius and M Roué and M Chinain and P Hess}, year = {2018}, date = {2018-01-01}, journal = {J Chromatogr A}, volume = {1571}, pages = {16-28}, abstract = {Ciguatera Fish Poisoning (CFP) is primarily caused by consumption of tropical and sub-tropical fish contaminated by Ciguatoxins (CTXs). These lipid-soluble, polyether neurotoxins are produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. While there is no regulatory level in Europe for CTXs, the European Food Safety Authority (EFSA) adopted the United States guidance level of 0.01?µg P-CTX1B eq.kg-1 of fish. This limit is extremely low and requires significant improvement in the detection of CTXs. In this study, we compared analytical protocols based on liquid chromatography coupled to tandem low or high resolution mass spectrometry (LC-LRMS or HRMS) to find the best conditions for sensitivity and/or selectivity. Different approaches such as LC conditions, ion choice and acquisition modes, were evaluated to detect the Pacific-ciguatoxins (P-CTXs) on a triple quadrupole (API4000 Qtrap, Sciex) or a quadrupole time of flight (QTOF 6550, Agilent Technologies) spectrometer. Moreover, matrix effects were calculated using matrix-matched calibration solutions of P-CTX1B and P-CTX3C prepared in purified fish extract. Subsequently, the method performance was assessed on naturally contaminated samples of seafood and phytoplankton. With LRMS, the ammoniated adduct ion used as a precursor ion showed an advantage for selectivity through confirmatory transitions, without affecting signal-to-noise ratios, and hence limits of detection (LODs). As also reported by some studies in the literature, methanol-based mobile phase gave better selectivity and sensitivity for the detection of P-CTXs. While the LOD for P-CTX1B and P-CTX3C met the EFSA recommendation level when using LRMS, the findings suggested careful evaluation of instrumental parameters for determination of CTXs. LODs were significantly higher for HRMS, which currently results in the need for a significantly higher sample intake. Nevertheless, HRMS allowed for the identification of artefacts and may allow for improved confirmation of the identity of P-CTXs analogues. Consequently, LRMS and HRMS are considered complementary to ensure adequate quantitation and identification of P-CTXs.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatera Fish Poisoning (CFP) is primarily caused by consumption of tropical and sub-tropical fish contaminated by Ciguatoxins (CTXs). These lipid-soluble, polyether neurotoxins are produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. While there is no regulatory level in Europe for CTXs, the European Food Safety Authority (EFSA) adopted the United States guidance level of 0.01?µg P-CTX1B eq.kg-1 of fish. This limit is extremely low and requires significant improvement in the detection of CTXs. In this study, we compared analytical protocols based on liquid chromatography coupled to tandem low or high resolution mass spectrometry (LC-LRMS or HRMS) to find the best conditions for sensitivity and/or selectivity. Different approaches such as LC conditions, ion choice and acquisition modes, were evaluated to detect the Pacific-ciguatoxins (P-CTXs) on a triple quadrupole (API4000 Qtrap, Sciex) or a quadrupole time of flight (QTOF 6550, Agilent Technologies) spectrometer. Moreover, matrix effects were calculated using matrix-matched calibration solutions of P-CTX1B and P-CTX3C prepared in purified fish extract. Subsequently, the method performance was assessed on naturally contaminated samples of seafood and phytoplankton. With LRMS, the ammoniated adduct ion used as a precursor ion showed an advantage for selectivity through confirmatory transitions, without affecting signal-to-noise ratios, and hence limits of detection (LODs). As also reported by some studies in the literature, methanol-based mobile phase gave better selectivity and sensitivity for the detection of P-CTXs. While the LOD for P-CTX1B and P-CTX3C met the EFSA recommendation level when using LRMS, the findings suggested careful evaluation of instrumental parameters for determination of CTXs. LODs were significantly higher for HRMS, which currently results in the need for a significantly higher sample intake. Nevertheless, HRMS allowed for the identification of artefacts and may allow for improved confirmation of the identity of P-CTXs analogues. Consequently, LRMS and HRMS are considered complementary to ensure adequate quantitation and identification of P-CTXs. |
| 38. | Zacares, M; Salvador-Herranz, G; Almenar, D; Tur, C; Argiles, R; Bourtzis, K; Bossin, H C; Pla, I: Exploring the potential of computer vision analysis of pupae size dimorphism for adaptive sex sorting systems of various vector mosquito species.. Dans: Parasite Vectors, 11 , p. 656, 2018. (type: Article de journal | Résumé | BibTeX) @article{5806, title = {Exploring the potential of computer vision analysis of pupae size dimorphism for adaptive sex sorting systems of various vector mosquito species.}, author = {M Zacares and G Salvador-Herranz and D Almenar and C Tur and R Argiles and K Bourtzis and H C Bossin and I Pla}, year = {2018}, date = {2018-01-01}, journal = {Parasite Vectors}, volume = {11}, pages = {656}, abstract = {BackgroundSeveral mosquito population suppression strategies based on the rearing and release of sterile males have provided promising results. However, the lack of an efficient male selection method has hampered the expansion of these approaches into large-scale operational programmes. Currently, most of these programmes targeting Aedes mosquitoes rely on sorting methods based on the sexual size dimorphism (SSD) at the pupal stage. The currently available sorting methods have not been developed based on biometric analysis, and there is therefore potential for improvement. We applied an automated pupal size estimator developed by Grupo Tragsa with laboratory samples of Anopheles arabiensis, Aedes albopictus, Ae. polynesiensis, and three strains of Ae. aegypti. The frequency distribution of the pupal size was analyzed. We propose a general model for the analysis of the frequency distribution of mosquito pupae in the context of SSD-sorting methods, which is based on a Gaussian mixture distribution functions, thus making possible the analysis of performance (% males recovery) and purity (% males on the sorted sample). Results For the three Aedes species, the distribution of the pupae size can be modeled by a mixture of two Gaussian distribution functions and the proposed model fitted the experimental data. For a given population, each size threshold is linked to a specific outcome of male recovery. Two dimensionless parameters that measure the suitability for SSD-based sorting of a specific batch of pupae are provided. The optimal sorting results are predicted for the highest values of SSD and lowest values of intra-batch variance. Rearing conditions have a strong influence in the performance of the SSD-sorting methods and non-standard rearing can lead to increase pupae size heterogeneity. Conclusions Sex sorting of pupae based on size dimorphism can be achieved with a high performance (% males recovery) and a reasonably high purity (% males on the sorted sample) for the different Aedes species and strains. The purity and performance of a sex sorting operation in the tested Aedes species are linked parameters whose relation can be modeled. The conclusions of this analysis are applicable to all the existing SSD-sorting methods. The efficiency of the SSD-sorting methods can be improved by reducing the heterogeneity of pupae size within rearing containers. The heterogeneity between batches does not strongly affect the quality of the sex sorting, as long as a specific separation threshold is not pre-set before the sorting process. For new developments, we recommend using adaptive and precise threshold selection methods applied individually to each batch or to a mix of batches. Adaptive and precise thresholds will allow the sex-sorting of mixed batches in operational conditions maintaining the target purity at the cost of a reduction in performance. We also recommend a strategy whereby an acceptable level of purity is pre-selected and remains constant across the different batches of pupae while the performance varies from batch to batch to fit with the desired purity.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundSeveral mosquito population suppression strategies based on the rearing and release of sterile males have provided promising results. However, the lack of an efficient male selection method has hampered the expansion of these approaches into large-scale operational programmes. Currently, most of these programmes targeting Aedes mosquitoes rely on sorting methods based on the sexual size dimorphism (SSD) at the pupal stage. The currently available sorting methods have not been developed based on biometric analysis, and there is therefore potential for improvement. We applied an automated pupal size estimator developed by Grupo Tragsa with laboratory samples of Anopheles arabiensis, Aedes albopictus, Ae. polynesiensis, and three strains of Ae. aegypti. The frequency distribution of the pupal size was analyzed. We propose a general model for the analysis of the frequency distribution of mosquito pupae in the context of SSD-sorting methods, which is based on a Gaussian mixture distribution functions, thus making possible the analysis of performance (% males recovery) and purity (% males on the sorted sample). Results For the three Aedes species, the distribution of the pupae size can be modeled by a mixture of two Gaussian distribution functions and the proposed model fitted the experimental data. For a given population, each size threshold is linked to a specific outcome of male recovery. Two dimensionless parameters that measure the suitability for SSD-based sorting of a specific batch of pupae are provided. The optimal sorting results are predicted for the highest values of SSD and lowest values of intra-batch variance. Rearing conditions have a strong influence in the performance of the SSD-sorting methods and non-standard rearing can lead to increase pupae size heterogeneity. Conclusions Sex sorting of pupae based on size dimorphism can be achieved with a high performance (% males recovery) and a reasonably high purity (% males on the sorted sample) for the different Aedes species and strains. The purity and performance of a sex sorting operation in the tested Aedes species are linked parameters whose relation can be modeled. The conclusions of this analysis are applicable to all the existing SSD-sorting methods. The efficiency of the SSD-sorting methods can be improved by reducing the heterogeneity of pupae size within rearing containers. The heterogeneity between batches does not strongly affect the quality of the sex sorting, as long as a specific separation threshold is not pre-set before the sorting process. For new developments, we recommend using adaptive and precise threshold selection methods applied individually to each batch or to a mix of batches. Adaptive and precise thresholds will allow the sex-sorting of mixed batches in operational conditions maintaining the target purity at the cost of a reduction in performance. We also recommend a strategy whereby an acceptable level of purity is pre-selected and remains constant across the different batches of pupae while the performance varies from batch to batch to fit with the desired purity. |
| 39. | Marie, J; Perera, D; Garstang, H; Bossin, H C; Bourtzis, K: Exploring Mosquito Fauna of Majuro Atoll (Republic of Marshall Islands) in the Context of Zika Outbreak.. Dans: J Med Entomol, 55 , p. 1299-1306, 2018. (type: Article de journal | Résumé | BibTeX) @article{5568, title = {Exploring Mosquito Fauna of Majuro Atoll (Republic of Marshall Islands) in the Context of Zika Outbreak.}, author = {J Marie and D Perera and H Garstang and H C Bossin and K Bourtzis}, year = {2018}, date = {2018-01-01}, journal = {J Med Entomol}, volume = {55}, pages = {1299-1306}, abstract = {First autochthonous Zika clinical case was reported in the Republic of Marshall Islands (RMI) on Majuro Atoll in February 2016. An entomological survey of mosquito larvae and adult populations was carried out in four areas of Majuro, the most populated atoll of RMI encompassing different habitats (forest, rural, or urban) including some with confirmed clinical Zika cases to evaluate which mosquito species could be involved in the Zika transmission. A total of 2,367 immature and adult mosquito specimens were collected and identified to the species level. In total, five mosquito species were detected, Aedes aegypti (Linnaeus), Aedes albopictus (Skuse), Aedes marshallensis (Stone and Bohart), Culex quinquefasciatus (Say), and Culex annulirostris (Skuse) (Diptera: Culicidae), a first record for RMI. The most abundant species was Ae. aegypti presumed to be the main vector of Zika virus followed by Ae. albopictus. Improved management of breeding containers through better public awareness and community engagement, mosquito surveillance and innovative mosquito control strategies using the sterile insect technique (SIT) and/or the incompatible insect technique (IIT) could help prevent outbreaks of arboviruses in the RMI.}, keywords = {}, pubstate = {published}, tppubtype = {article} } First autochthonous Zika clinical case was reported in the Republic of Marshall Islands (RMI) on Majuro Atoll in February 2016. An entomological survey of mosquito larvae and adult populations was carried out in four areas of Majuro, the most populated atoll of RMI encompassing different habitats (forest, rural, or urban) including some with confirmed clinical Zika cases to evaluate which mosquito species could be involved in the Zika transmission. A total of 2,367 immature and adult mosquito specimens were collected and identified to the species level. In total, five mosquito species were detected, Aedes aegypti (Linnaeus), Aedes albopictus (Skuse), Aedes marshallensis (Stone and Bohart), Culex quinquefasciatus (Say), and Culex annulirostris (Skuse) (Diptera: Culicidae), a first record for RMI. The most abundant species was Ae. aegypti presumed to be the main vector of Zika virus followed by Ae. albopictus. Improved management of breeding containers through better public awareness and community engagement, mosquito surveillance and innovative mosquito control strategies using the sterile insect technique (SIT) and/or the incompatible insect technique (IIT) could help prevent outbreaks of arboviruses in the RMI. |
| 40. | Clausing, R J; Losen, B; Oberhaensli, F R; Darius, H T; Sibat, M; Hess, P; Swarzenski, P W; Chinain, M; Bottein, Dechraoui M Y: Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish.. Dans: Aquatic Toxicol, 200 , p. 257-65, 2018. (type: Article de journal | Résumé | BibTeX) @article{5562, title = {Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish.}, author = {R J Clausing and B Losen and F R Oberhaensli and H T Darius and M Sibat and P Hess and P W Swarzenski and M Chinain and M Y Dechraoui Bottein}, year = {2018}, date = {2018-01-01}, journal = {Aquatic Toxicol}, volume = {200}, pages = {257-65}, abstract = {Ciguatoxins (CTXs) are potent algal toxins that cause widespread ciguatera poisoning and are found ubiquitously in coral reef food webs. Here we developed an environmentally-relevant, experimental model of CTX trophic transfer involving dietary exposure of herbivorous fish to the CTX-producing microalgae Gambierdiscus polynesiensis. Juvenile Naso brevirostris were fed a gel-food embedded with microalgae for 16 weeks (89 cells g-1 fish daily, 0.4mg CTX3C equiv kg-1 fish). CTXs in muscle tissue were detectable after 2 weeks at levels above the threshold for human intoxication (1.2 ± mg CTX3C equiv kg-1). Although tissue CTX concentrations stabilized after 8 weeks (~3 ± 0.5 mg CTX3C equiv kg-1), muscle toxin burden (total ìg CTX in muscle tissue) continued to increase linearly through the end of the experiment (16 weeks). Toxin accumulation was therefore continuous, yet masked by somatic growth dilution. The observed CTX concentrations, accumulation rates, and general absence of behavioural signs of intoxication are consistent with field observations and indicate that this method of dietary exposure may be used to develop predictive models of tissue-specific CTX uptake, metabolism and depuration. Results also imply that slow-growing fish may accumulate higher CTX flesh concentrations than fast-growing fish, which has important implications for global seafood safety.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatoxins (CTXs) are potent algal toxins that cause widespread ciguatera poisoning and are found ubiquitously in coral reef food webs. Here we developed an environmentally-relevant, experimental model of CTX trophic transfer involving dietary exposure of herbivorous fish to the CTX-producing microalgae Gambierdiscus polynesiensis. Juvenile Naso brevirostris were fed a gel-food embedded with microalgae for 16 weeks (89 cells g-1 fish daily, 0.4mg CTX3C equiv kg-1 fish). CTXs in muscle tissue were detectable after 2 weeks at levels above the threshold for human intoxication (1.2 ± mg CTX3C equiv kg-1). Although tissue CTX concentrations stabilized after 8 weeks (~3 ± 0.5 mg CTX3C equiv kg-1), muscle toxin burden (total ìg CTX in muscle tissue) continued to increase linearly through the end of the experiment (16 weeks). Toxin accumulation was therefore continuous, yet masked by somatic growth dilution. The observed CTX concentrations, accumulation rates, and general absence of behavioural signs of intoxication are consistent with field observations and indicate that this method of dietary exposure may be used to develop predictive models of tissue-specific CTX uptake, metabolism and depuration. Results also imply that slow-growing fish may accumulate higher CTX flesh concentrations than fast-growing fish, which has important implications for global seafood safety. |
| 41. | Mueller, C; Cao-Lormeau, V M: Insect-borne viruses and host skin interface.. Dans: Skin and arthropod vectors, Academic P , p. 275-92, Boulanger N., 2018. (type: Book Chapter | BibTeX) @inbook{5804, title = {Insect-borne viruses and host skin interface.}, author = {C Mueller and V M Cao-Lormeau}, year = {2018}, date = {2018-01-01}, booktitle = {Skin and arthropod vectors}, volume = {Academic P}, pages = {275-92}, publisher = {Boulanger N.}, keywords = {}, pubstate = {published}, tppubtype = {inbook} } |
| 42. | Musso, D; Cao-Lormeau, V M: Is the Zika threat over?. Dans: Clin Microbiol Infect, 24 , p. 566-7, 2018. (type: Article de journal | BibTeX) @article{5575, title = {Is the Zika threat over?}, author = {D Musso and V M Cao-Lormeau}, year = {2018}, date = {2018-01-01}, journal = {Clin Microbiol Infect}, volume = {24}, pages = {566-7}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 43. | Musso, D: Infections in French Polynesia.. Dans: New Microbes New Infections, 27 , p. 27-8, 2018. (type: Article de journal | BibTeX) @article{5574, title = {Infections in French Polynesia.}, author = {D Musso}, year = {2018}, date = {2018-01-01}, journal = {New Microbes New Infections}, volume = {27}, pages = {27-8}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 44. | Hardison, D R; Holland, W C; Darius, H T; Chinain, M; Tester, P A; Shea, D; Bogdanoff, A K; Morris, Jr. J A; Quintana, Flores H A; Christopher, R: Investigation of ciguatoxins in invasive lionfish from the greater caribbean region: Implications for fishery development.. Dans: PLOS One, 13 , p. e0198358, 2018. (type: Article de journal | Résumé | BibTeX) @article{5566, title = {Investigation of ciguatoxins in invasive lionfish from the greater caribbean region: Implications for fishery development.}, author = {D R Hardison and W C Holland and H T Darius and M Chinain and P A Tester and D Shea and A K Bogdanoff and Jr. J A Morris and H A Flores Quintana and R Christopher}, year = {2018}, date = {2018-01-01}, journal = {PLOS One}, volume = {13}, pages = {e0198358}, abstract = {Lionfish, native to reef ecosystems of the tropical and sub-tropical Indo-Pacific, were introduced to Florida waters in the 1980s, and have spread rapidly throughout the northwestern Atlantic, Caribbean Sea and the Gulf of Mexico. These invasive, carnivorous fish significantly reduce other fish and benthic invertebrate biomass, fish recruitment, and species richness in reef ecosystems. Fisheries resource managers have proposed the establishment of a commercial fishery to reduce lionfish populations and mitigate adverse effects on reef communities. The potential for a commercial fishery for lionfish is the primary reason to identify locations where lionfish accumulate sufficient amounts of ciguatoxin (CTX) to cause ciguatera fish poisoning (CFP), the leading cause of non-bacterial seafood poisoning associated with fish consumption. To address this issue, an initial geographic assessment of CTX toxicity in lionfish from the Caribbean and Gulf of Mexico was conducted. Lionfish samples (n = 293) were collected by spearfishing from 13 locations (74 sampling sites) around the Caribbean and Gulf of Mexico between 2012 and 2015. The highest frequencies of lionfish containing measurable CTX occurred in areas known to be high-risk regions for CFP in the central to eastern Caribbean (e.g., 53% British Virgin Islands and 5% Florida Keys). Though measurable CTX was found in some locations, the majority of the samples (99.3%) contained CTX concentrations below the United States Food and Drug Administration guidance level of 0.1 ppb Caribbean ciguatoxin-1 (C-CTX-1) equivalents (eq.). Only 0.7% of lionfish tested contained more than 0.1 ppb C-CTX-1 eq. As of 2018, there has been one suspected case of CFP from eating lionfish. Given this finding, current risk reduction techniques used to manage CTX accumulating fish are discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Lionfish, native to reef ecosystems of the tropical and sub-tropical Indo-Pacific, were introduced to Florida waters in the 1980s, and have spread rapidly throughout the northwestern Atlantic, Caribbean Sea and the Gulf of Mexico. These invasive, carnivorous fish significantly reduce other fish and benthic invertebrate biomass, fish recruitment, and species richness in reef ecosystems. Fisheries resource managers have proposed the establishment of a commercial fishery to reduce lionfish populations and mitigate adverse effects on reef communities. The potential for a commercial fishery for lionfish is the primary reason to identify locations where lionfish accumulate sufficient amounts of ciguatoxin (CTX) to cause ciguatera fish poisoning (CFP), the leading cause of non-bacterial seafood poisoning associated with fish consumption. To address this issue, an initial geographic assessment of CTX toxicity in lionfish from the Caribbean and Gulf of Mexico was conducted. Lionfish samples (n = 293) were collected by spearfishing from 13 locations (74 sampling sites) around the Caribbean and Gulf of Mexico between 2012 and 2015. The highest frequencies of lionfish containing measurable CTX occurred in areas known to be high-risk regions for CFP in the central to eastern Caribbean (e.g., 53% British Virgin Islands and 5% Florida Keys). Though measurable CTX was found in some locations, the majority of the samples (99.3%) contained CTX concentrations below the United States Food and Drug Administration guidance level of 0.1 ppb Caribbean ciguatoxin-1 (C-CTX-1) equivalents (eq.). Only 0.7% of lionfish tested contained more than 0.1 ppb C-CTX-1 eq. As of 2018, there has been one suspected case of CFP from eating lionfish. Given this finding, current risk reduction techniques used to manage CTX accumulating fish are discussed. |
| 45. | Richard, V; Aubry, M: Method for Simple and Rapid Concentration of Zika Virus Particles from Infected Cell-Culture Supernatants.. Dans: J Virol Methods, 255 , p. 82-3, 2018. (type: Article de journal | Résumé | BibTeX) @article{5581, title = {Method for Simple and Rapid Concentration of Zika Virus Particles from Infected Cell-Culture Supernatants.}, author = {V Richard and M Aubry}, year = {2018}, date = {2018-01-01}, journal = {J Virol Methods}, volume = {255}, pages = {82-3}, abstract = {Experimental studies on Zika virus (ZIKV) may require improvement of infectious titers in viral stocks obtained by cell culture amplification. The use of centrifugal filter devices to increase infectious titers of ZIKV from cell-culture supernatants is highlighted here. A mean gain of 2.33?±?0.12 log10 DICT50/mL was easily and rapidly obtained with this process. This efficient method of ultrafiltration may be applied to other viruses and be useful in various experimental studies requiring high viral titers.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Experimental studies on Zika virus (ZIKV) may require improvement of infectious titers in viral stocks obtained by cell culture amplification. The use of centrifugal filter devices to increase infectious titers of ZIKV from cell-culture supernatants is highlighted here. A mean gain of 2.33?±?0.12 log10 DICT50/mL was easily and rapidly obtained with this process. This efficient method of ultrafiltration may be applied to other viruses and be useful in various experimental studies requiring high viral titers. |
| 46. | Pettersson, J; Bohlin, J; Dupont-Rouzeyrol, M; Brynildsrud, O; Alfsnes, K; Cao-Lormeau, V M; Gaunt, M W; Falconar, A K; Lamballerie, De X; Eldholm, V; Musso, D; Gould, E: Re-visiting the evolution, dispersal and epidemiology of Zika virus in Asia.. Dans: Emerg Infect Dis, 7 , p. 79, 2018. (type: Article de journal | Résumé | BibTeX) @article{5580, title = {Re-visiting the evolution, dispersal and epidemiology of Zika virus in Asia.}, author = {J Pettersson and J Bohlin and M Dupont-Rouzeyrol and O Brynildsrud and K Alfsnes and V M Cao-Lormeau and M W Gaunt and A K Falconar and X De Lamballerie and V Eldholm and D Musso and E Gould}, year = {2018}, date = {2018-01-01}, journal = {Emerg Infect Dis}, volume = {7}, pages = {79}, abstract = {Based on serological evidence and viral isolation, Zika virus (ZIKV) has circulated for many years relatively benignly in a sylvatic cycle in Africa and an urban cycle in South East Asia (SEA). With the recent availability of limited but novel Indian ZIKV sequences to add to the plethora of SEA sequences, we traced the phylogenetic history and spatio-temporal dispersal pattern of ZIKV in Asia prior to its explosive emergence in the Pacific region and the Americas. These analyses demonstrated that the introduction and dispersal of ZIKV on the Pacific islands were preceded by an extended period of relatively silent transmission in SEA, enabling the virus to expand geographically and evolve adaptively before its unanticipated introduction to immunologically naive populations on the Pacific islands and in the Americas. Our findings reveal new features of the evolution and dispersal of this intriguing virus and may benefit future disease control strategies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Based on serological evidence and viral isolation, Zika virus (ZIKV) has circulated for many years relatively benignly in a sylvatic cycle in Africa and an urban cycle in South East Asia (SEA). With the recent availability of limited but novel Indian ZIKV sequences to add to the plethora of SEA sequences, we traced the phylogenetic history and spatio-temporal dispersal pattern of ZIKV in Asia prior to its explosive emergence in the Pacific region and the Americas. These analyses demonstrated that the introduction and dispersal of ZIKV on the Pacific islands were preceded by an extended period of relatively silent transmission in SEA, enabling the virus to expand geographically and evolve adaptively before its unanticipated introduction to immunologically naive populations on the Pacific islands and in the Americas. Our findings reveal new features of the evolution and dispersal of this intriguing virus and may benefit future disease control strategies. |
| 47. | Vielle, N J; Zumkehr, B; García-Nicolás, O; Stojanov, M; Baud, D; Musso, D; Summerfield, A; Alves, M P: Silent infection of human dendritic cells by African and Asian strains of Zika virus.. Dans: Sci Rep, 8 , p. 5440, 2018. (type: Article de journal | Résumé | BibTeX) @article{5588, title = {Silent infection of human dendritic cells by African and Asian strains of Zika virus.}, author = {N J Vielle and B Zumkehr and O García-Nicolás and M Stojanov and D Baud and D Musso and A Summerfield and M P Alves}, year = {2018}, date = {2018-01-01}, journal = {Sci Rep}, volume = {8}, pages = {5440}, abstract = {While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made.}, keywords = {}, pubstate = {published}, tppubtype = {article} } While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made. |
| 48. | Seck, E H; Senghor, B; Merhej, V; Bachar, D; Cadoret, F; Robert, C; Azhar, E I; Yasir, M; Bibi, F; Jiman-Fatani, A; Konate, Salimata D; Musso, D; Doumbo, O; Sokhna, C; Levasseur, A; Lagier, J; Khelaifia, S; Million, M; Raoult, D: Salt in Stools is associated with Obesity, Gut Halophilic Microbiota and Akkermansia muciniphila depletion in Humans.. Dans: Int J Obes, 43 , p. 862-871, 2018. (type: Article de journal | BibTeX) @article{5585, title = {Salt in Stools is associated with Obesity, Gut Halophilic Microbiota and Akkermansia muciniphila depletion in Humans.}, author = {E H Seck and B Senghor and V Merhej and D Bachar and F Cadoret and C Robert and E I Azhar and M Yasir and F Bibi and A Jiman-Fatani and D Salimata Konate and D Musso and O Doumbo and C Sokhna and A Levasseur and J Lagier and S Khelaifia and M Million and D Raoult}, year = {2018}, date = {2018-01-01}, journal = {Int J Obes}, volume = {43}, pages = {862-871}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 49. | Roué, M; Darius, H T; Chinain, M: Solid Phase Adsorption Toxin Tracking (SPATT) Technology for the Monitoring of Aquatic Toxins: A Review.. Dans: Toxins, 10 , p. pii: E167, 2018. (type: Article de journal | Résumé | BibTeX) @article{5582, title = {Solid Phase Adsorption Toxin Tracking (SPATT) Technology for the Monitoring of Aquatic Toxins: A Review.}, author = {M Roué and H T Darius and M Chinain}, year = {2018}, date = {2018-01-01}, journal = {Toxins}, volume = {10}, pages = {pii: E167}, abstract = {The Solid Phase Adsorption Toxin Tracking (SPATT) technology, first introduced in 2004, uses porous synthetic resins capable of passively adsorbing toxins produced by harmful microalgae or cyanobacteria and dissolved in the water. This method allows for the detection of toxic compounds directly in the water column and offers numerous advantages over current monitoring techniques (e.g., shellfish or fish testing and microalgae/cyanobacteria cell detection), despite some limitations. Numerous laboratory and field studies, testing different adsorbent substrates of which Diaion® HP20 resin appears to be the most versatile substrate, have been carried out worldwide to assess the applicability of these passive monitoring devices to the detection of toxins produced by a variety of marine and freshwater microorganisms. SPATT technology has been shown to provide reliable, sensitive and time-integrated sampling of various aquatic toxins, and also has the potential to provide an early warning system for both the occurrence of toxic microalgae or cyanobacteria and bioaccumulation of toxins in foodstuffs. This review describes the wide range of lipophilic and hydrophilic toxins associated with toxin-producing harmful algal blooms (HABs) that are successfully detected by SPATT devices. Implications in terms of monitoring of emerging toxic risks and reinforcement of current risk assessment programs are also discussed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Solid Phase Adsorption Toxin Tracking (SPATT) technology, first introduced in 2004, uses porous synthetic resins capable of passively adsorbing toxins produced by harmful microalgae or cyanobacteria and dissolved in the water. This method allows for the detection of toxic compounds directly in the water column and offers numerous advantages over current monitoring techniques (e.g., shellfish or fish testing and microalgae/cyanobacteria cell detection), despite some limitations. Numerous laboratory and field studies, testing different adsorbent substrates of which Diaion® HP20 resin appears to be the most versatile substrate, have been carried out worldwide to assess the applicability of these passive monitoring devices to the detection of toxins produced by a variety of marine and freshwater microorganisms. SPATT technology has been shown to provide reliable, sensitive and time-integrated sampling of various aquatic toxins, and also has the potential to provide an early warning system for both the occurrence of toxic microalgae or cyanobacteria and bioaccumulation of toxins in foodstuffs. This review describes the wide range of lipophilic and hydrophilic toxins associated with toxin-producing harmful algal blooms (HABs) that are successfully detected by SPATT devices. Implications in terms of monitoring of emerging toxic risks and reinforcement of current risk assessment programs are also discussed. |
| 50. | Cardona-Ospina, J A; Alvarado-Arnez, L E; Escalera-Antezana, Juan Pablo J P; Bandeira, A C; Musso, D; Rodriguez-Morales, A J: Sexual transmission of arboviruses: more to explore?. Dans: Int J Infect Dis, 76 , p. 126-127, 2018. (type: Article de journal | BibTeX) @article{5573, title = {Sexual transmission of arboviruses: more to explore?}, author = {J A Cardona-Ospina and L E Alvarado-Arnez and J P Juan Pablo Escalera-Antezana and A C Bandeira and D Musso and A J Rodriguez-Morales}, year = {2018}, date = {2018-01-01}, journal = {Int J Infect Dis}, volume = {76}, pages = {126-127}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 51. | Mathieu-Daudé, F; Claverie, A; Plichart, C; Boulanger, D; Mphande, F A; Bossin, H C: Specific human antibody responses to Aedes aegypti and Aedes polynesiensis saliva: a new epidemiological tool to assess human exposure to disease vectors in the Pacific.. Dans: Plos Neg Trop Dis, 12 , p. e0006660, 2018. (type: Article de journal | Résumé | BibTeX) @article{5570, title = {Specific human antibody responses to Aedes aegypti and Aedes polynesiensis saliva: a new epidemiological tool to assess human exposure to disease vectors in the Pacific.}, author = {F Mathieu-Daudé and A Claverie and C Plichart and D Boulanger and F A Mphande and H C Bossin}, year = {2018}, date = {2018-01-01}, journal = {Plos Neg Trop Dis}, volume = {12}, pages = {e0006660}, abstract = {BackgroundAedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. In French Polynesia, Aedes aegypti is the main vector of dengue, chikungunya and Zika, and Aedes polynesiensis the primary vector of Bancroftian filariasis and a secondary vector of arboviruses. Tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. A promising approach to quantify the human-vector contact relies on the detection and the quantification of antibodies directed against mosquito salivary proteins. Methodology/Principal findings An ELISA test was developed to detect and quantify the presence of immunoglobulin G (IgG) directed against proteins from salivary gland extracts (SGE) of Ae. aegypti and Ae. polynesiensis in human populations exposed to either species, through a cross-sectional study. In Tahiti and Moorea islands where Ae. aegypti and Ae. polynesiensis are present, the test revealed that 98% and 68% of individuals have developed IgG directed against Ae. aegypti and Ae. polynesiensis SGE, respectively. By comparison, ELISA tests conducted on a cohort of people from metropolitan France, not exposed to these Aedes mosquitoes, indicated that 97% of individuals had no IgG directed against SGE of either mosquito species. The analysis of additional cohorts representing different entomological Aedes contexts showed no ELISA IgG cross-reactivity between Ae. aegypti and Ae. polynesiensis SGE. Conclusions/Significance The IgG response to salivary gland extracts seems to be a valid and specific biomarker of human exposure to the bites of Ae. aegypti and Ae. polynesiensis. This new immuno-epidemiological tool will enhance our understanding of people exposure to mosquito bites, facilitate the identification of areas where disease transmission risk is high and permit to evaluate the efficacy of novel vector control strategies in Pacific islands and other tropical settings. Author summary In Pacific islands like in most tropical regions, Aedes mosquitoes affect the health of human populations by transmitting diseases like dengue, chikungunya, Zika and filariasis. The biting nuisance of Aedes mosquitoes also impacts local tourism, affecting the sustainability of island economies. Mosquito saliva is injected during the biting process, and the response triggered by the human immune system to proteins contained in mosquito saliva was shown to be a relevant biomarker of exposure to mosquito bites. Using this approach, we have developed an immuno-epidemiological tool to investigate the exposure of people to the bites of Aedes aegypti and Aedes polynesiensis, two significant mosquito vectors of infectious diseases in French Polynesia and other island countries and territories in the Pacific. This novel tool proved specific and reliable. It will improve the assessment of disease transmission risk and be useful for measuring the efficacy of both conventional and innovative vector control strategies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundAedes mosquitoes severely affect the health and wellbeing of human populations by transmitting infectious diseases. In French Polynesia, Aedes aegypti is the main vector of dengue, chikungunya and Zika, and Aedes polynesiensis the primary vector of Bancroftian filariasis and a secondary vector of arboviruses. Tools for assessing the risk of disease transmission or for measuring the efficacy of vector control programmes are scarce. A promising approach to quantify the human-vector contact relies on the detection and the quantification of antibodies directed against mosquito salivary proteins. Methodology/Principal findings An ELISA test was developed to detect and quantify the presence of immunoglobulin G (IgG) directed against proteins from salivary gland extracts (SGE) of Ae. aegypti and Ae. polynesiensis in human populations exposed to either species, through a cross-sectional study. In Tahiti and Moorea islands where Ae. aegypti and Ae. polynesiensis are present, the test revealed that 98% and 68% of individuals have developed IgG directed against Ae. aegypti and Ae. polynesiensis SGE, respectively. By comparison, ELISA tests conducted on a cohort of people from metropolitan France, not exposed to these Aedes mosquitoes, indicated that 97% of individuals had no IgG directed against SGE of either mosquito species. The analysis of additional cohorts representing different entomological Aedes contexts showed no ELISA IgG cross-reactivity between Ae. aegypti and Ae. polynesiensis SGE. Conclusions/Significance The IgG response to salivary gland extracts seems to be a valid and specific biomarker of human exposure to the bites of Ae. aegypti and Ae. polynesiensis. This new immuno-epidemiological tool will enhance our understanding of people exposure to mosquito bites, facilitate the identification of areas where disease transmission risk is high and permit to evaluate the efficacy of novel vector control strategies in Pacific islands and other tropical settings. Author summary In Pacific islands like in most tropical regions, Aedes mosquitoes affect the health of human populations by transmitting diseases like dengue, chikungunya, Zika and filariasis. The biting nuisance of Aedes mosquitoes also impacts local tourism, affecting the sustainability of island economies. Mosquito saliva is injected during the biting process, and the response triggered by the human immune system to proteins contained in mosquito saliva was shown to be a relevant biomarker of exposure to mosquito bites. Using this approach, we have developed an immuno-epidemiological tool to investigate the exposure of people to the bites of Aedes aegypti and Aedes polynesiensis, two significant mosquito vectors of infectious diseases in French Polynesia and other island countries and territories in the Pacific. This novel tool proved specific and reliable. It will improve the assessment of disease transmission risk and be useful for measuring the efficacy of both conventional and innovative vector control strategies. |
| 52. | Aubry, M; Teissier, A; Huart, M; Merceron, S; Vanhomwegen, J; Mapotoeke, M; Mariteragi-Helle, T; Roche, C; Vial, A L; Teururai, S; Sicard, S; Paulous, S; Despres, P; Manuguerra, J C; Mallet, H P; Imrie, A; Musso, D; Deparis, X; Cao-Lo, : Seroprevalence of dengue and chikungunya virus antibodies, French Polynesia, 2014-2015.. Dans: Emerg Infect Dis, 24 , p. 558-61, 2018. (type: Article de journal | Résumé | BibTeX) @article{5558, title = {Seroprevalence of dengue and chikungunya virus antibodies, French Polynesia, 2014-2015.}, author = {M Aubry and A Teissier and M Huart and S Merceron and J Vanhomwegen and M Mapotoeke and T Mariteragi-Helle and C Roche and A L Vial and S Teururai and S Sicard and S Paulous and P Despres and J C Manuguerra and H P Mallet and A Imrie and D Musso and X Deparis and Cao-Lo}, year = {2018}, date = {2018-01-01}, journal = {Emerg Infect Dis}, volume = {24}, pages = {558-61}, abstract = {We investigated dengue and chikungunya virus antibody seroprevalence in French Polynesia during 2014-2015. Dengue virus seroprevalence was ˜60% among schoolchildren and >83% among the general population; chikungunya virus seroprevalence was <3% before and 76% after Zika virus emergence (2013). Dengue virus herd immunity may affect Zika virus infection and pathogenesis.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We investigated dengue and chikungunya virus antibody seroprevalence in French Polynesia during 2014-2015. Dengue virus seroprevalence was ˜60% among schoolchildren and >83% among the general population; chikungunya virus seroprevalence was <3% before and 76% after Zika virus emergence (2013). Dengue virus herd immunity may affect Zika virus infection and pathogenesis. |
| 53. | Roué, M; Darius, H T; Ung, A; Viallon, J; Sibat, M; Hess, P; Amzil, Z; Chinain, M: Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis.. Dans: 10 , p. pii: E189, 2018. (type: Article de journal | Résumé | BibTeX) @article{5584, title = {Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis.}, author = {M Roué and H T Darius and A Ung and J Viallon and M Sibat and P Hess and Z Amzil and M Chinain}, year = {2018}, date = {2018-01-01}, volume = {10}, pages = {pii: E189}, abstract = {Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. All tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. In a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). Observations suggest that no toxin elimination is visible in T. maxima throughout 6 days of detoxification.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. All tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. In a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). Observations suggest that no toxin elimination is visible in T. maxima throughout 6 days of detoxification. |
| 54. | Gatti, C M; Lonati, D; Darius, H T; Zancan, A; Roué, M; Schicchi, A; Locatelli, C A; Chinain, M: Tectus niloticus (Tegulidae, Gastropod) as a novel vector of ciguatera poisoning: clinical characterization and follow up of a mass poisoning event in Nuku Hiva Island (French Polynesia).. Dans: Toxins, 10 , p. pii: E102, 2018. (type: Article de journal | Résumé | BibTeX) @article{5565, title = {Tectus niloticus (Tegulidae, Gastropod) as a novel vector of ciguatera poisoning: clinical characterization and follow up of a mass poisoning event in Nuku Hiva Island (French Polynesia).}, author = {C M Gatti and D Lonati and H T Darius and A Zancan and M Roué and A Schicchi and C A Locatelli and M Chinain}, year = {2018}, date = {2018-01-01}, journal = {Toxins}, volume = {10}, pages = {pii: E102}, abstract = {Ciguatera fish poisoning (CFP) is the most prevalent non-bacterial food-borne form of poisoning in French Polynesia, which results from the consumption of coral reef fish naturally contaminated with ciguatoxins produced by dinoflagellates in the genus Gambierdiscus. Since the early 2000s, this French territory has also witnessed the emergence of atypical forms of ciguatera, known as ciguatera shellfish poisoning (CSP), associated with the consumption of marine invertebrates. In June 2014, nine tourists simultaneously developed a major and persistent poisoning syndrome following the consumption of the gastropod Tectus niloticus collected in Anaho, a secluded bay of Nuku Hiva Island (Marquesas Archipelago, French Polynesia). The unusual nature and severity of this event prompted a multidisciplinary investigation in order to characterize the etiology and document the short/long-term health consequences of this mass-poisoning event. This paper presents the results of clinical investigations based on hospital medical records, medical follow-up conducted six and 20 months post-poisoning, including a case description. This study is the first to describe the medical signature of T. niloticus poisoning in French Polynesia and contributed to alerting local authorities about the potential health hazards associated with the consumption of this gastropod, which is highly prized by local communities in Pacific island countries and territories.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatera fish poisoning (CFP) is the most prevalent non-bacterial food-borne form of poisoning in French Polynesia, which results from the consumption of coral reef fish naturally contaminated with ciguatoxins produced by dinoflagellates in the genus Gambierdiscus. Since the early 2000s, this French territory has also witnessed the emergence of atypical forms of ciguatera, known as ciguatera shellfish poisoning (CSP), associated with the consumption of marine invertebrates. In June 2014, nine tourists simultaneously developed a major and persistent poisoning syndrome following the consumption of the gastropod Tectus niloticus collected in Anaho, a secluded bay of Nuku Hiva Island (Marquesas Archipelago, French Polynesia). The unusual nature and severity of this event prompted a multidisciplinary investigation in order to characterize the etiology and document the short/long-term health consequences of this mass-poisoning event. This paper presents the results of clinical investigations based on hospital medical records, medical follow-up conducted six and 20 months post-poisoning, including a case description. This study is the first to describe the medical signature of T. niloticus poisoning in French Polynesia and contributed to alerting local authorities about the potential health hazards associated with the consumption of this gastropod, which is highly prized by local communities in Pacific island countries and territories. |
| 55. | Darius, H T; Roué, M; Sibat, M; Viallon, J; Gatti, C M; Vandersea, M W; Tester, P A; Litaker, R W; Amzil, Z; Hess, P; Chinain, M: Tectus niloticus (Tegulidae, Gastropod), as a novel vector of ciguatera poisoning. Detection of Pacific ciguatoxins in toxic samples from Nuku Hiva Island (French Polynesia).. Dans: Toxins, 10 , p. pii: E2, 2018. (type: Article de journal | Résumé | BibTeX) @article{5564, title = {Tectus niloticus (Tegulidae, Gastropod), as a novel vector of ciguatera poisoning. Detection of Pacific ciguatoxins in toxic samples from Nuku Hiva Island (French Polynesia).}, author = {H T Darius and M Roué and M Sibat and J Viallon and C M Gatti and M W Vandersea and P A Tester and R W Litaker and Z Amzil and P Hess and M Chinain}, year = {2018}, date = {2018-01-01}, journal = {Toxins}, volume = {10}, pages = {pii: E2}, abstract = {Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. The report of a CFP-like mass-poisoning outbreak following the consumption of Tectus niloticus (Tegulidae, Gastropod) from Anaho Bay on Nuku Hiva Island (Marquesas archipelago, French Polynesia) prompted field investigations to assess the presence of CTXs in T. niloticus. Samples were collected from Anaho Bay, 1, 6 and 28 months after this poisoning outbreak, as well as in Taiohae and Taipivai bays. Toxicity analysis using the neuroblastoma cell-based assay (CBA-N2a) detected the presence of CTXs only in Anaho Bay T. niloticus samples. This is consistent with qPCR results on window screen samples indicating the presence of Gambierdiscus communities dominated by the species G. polynesiensis in Anaho Bay. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major congener, followed by P-CTX-3C, P-CTX-4A and P-CTX-4B in toxic samples. Between July 2014 and November 2016, toxin content in T. niloticus progressively decreased, but was consistently above the safety limit recommended for human consumption. This study confirms for the first time T. niloticus as a novel vector of CFP in French Polynesia.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. The report of a CFP-like mass-poisoning outbreak following the consumption of Tectus niloticus (Tegulidae, Gastropod) from Anaho Bay on Nuku Hiva Island (Marquesas archipelago, French Polynesia) prompted field investigations to assess the presence of CTXs in T. niloticus. Samples were collected from Anaho Bay, 1, 6 and 28 months after this poisoning outbreak, as well as in Taiohae and Taipivai bays. Toxicity analysis using the neuroblastoma cell-based assay (CBA-N2a) detected the presence of CTXs only in Anaho Bay T. niloticus samples. This is consistent with qPCR results on window screen samples indicating the presence of Gambierdiscus communities dominated by the species G. polynesiensis in Anaho Bay. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major congener, followed by P-CTX-3C, P-CTX-4A and P-CTX-4B in toxic samples. Between July 2014 and November 2016, toxin content in T. niloticus progressively decreased, but was consistently above the safety limit recommended for human consumption. This study confirms for the first time T. niloticus as a novel vector of CFP in French Polynesia. |
| 56. | Darius, H T; Roué, M; Sibat, M; Viallon, J; Gatti, C M; Vandersea, M W; Tester, P A; Litaker, R W; Amzil, Z; Hess, P; Chinain, M: Toxicological investigations on the sea urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): evidence for the presence of Pacific ciguatoxins.. Dans: Marine Drugs, 16 , p. 122, 2018. (type: Article de journal | Résumé | BibTeX) @article{5563, title = {Toxicological investigations on the sea urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): evidence for the presence of Pacific ciguatoxins.}, author = {H T Darius and M Roué and M Sibat and J Viallon and C M Gatti and M W Vandersea and P A Tester and R W Litaker and Z Amzil and P Hess and M Chinain}, year = {2018}, date = {2018-01-01}, journal = {Marine Drugs}, volume = {16}, pages = {122}, abstract = {The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cellbased assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic assemblages of Anaho Bay, and G. polynesiensis was highly prevalent in in vitro cultures according to qPCR results. Liquid chromatographytandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major ciguatoxin congener in toxic sea urchin samples, followed by 51-OH-P-CTX-3C, P-CTX-3C, P-CTX-4A, and P-CTX-4B. Between July 2015 and November 2016, the toxin content in T. gratilla decreased, but was consistently above the safety limit allowed for human consumption. This study provides evidence of CTX bioaccumulation in T. gratilla as a cause of ciguatera-like poisoning associated with a documented symptomatology.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cellbased assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic assemblages of Anaho Bay, and G. polynesiensis was highly prevalent in in vitro cultures according to qPCR results. Liquid chromatographytandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major ciguatoxin congener in toxic sea urchin samples, followed by 51-OH-P-CTX-3C, P-CTX-3C, P-CTX-4A, and P-CTX-4B. Between July 2015 and November 2016, the toxin content in T. gratilla decreased, but was consistently above the safety limit allowed for human consumption. This study provides evidence of CTX bioaccumulation in T. gratilla as a cause of ciguatera-like poisoning associated with a documented symptomatology. |
| 57. | Vouga, M; Musso, D; Goorhuis, A; Freedman, D O; Baud, D: Updated Zika virus recommendations are needed.. Dans: Lancet, 392 , p. 818-9, 2018. (type: Article de journal | BibTeX) @article{5589, title = {Updated Zika virus recommendations are needed.}, author = {M Vouga and D Musso and A Goorhuis and D O Freedman and D Baud}, year = {2018}, date = {2018-01-01}, journal = {Lancet}, volume = {392}, pages = {818-9}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 58. | Musso, D; Rodriguez-Morales, A J; Levi, J E; Cao-Lormeau, V M; Gubler, D J: Unexpected outbreaks of arbovirus infections: lessons learned from the Pacific and tropical America.. Dans: Lancet Infect Dis, 18 , p. e355-e361, 2018. (type: Article de journal | Résumé | BibTeX) @article{5578, title = {Unexpected outbreaks of arbovirus infections: lessons learned from the Pacific and tropical America.}, author = {D Musso and A J Rodriguez-Morales and J E Levi and V M Cao-Lormeau and D J Gubler}, year = {2018}, date = {2018-01-01}, journal = {Lancet Infect Dis}, volume = {18}, pages = {e355-e361}, abstract = {Pandemic arboviruses have emerged as a major global health problem in the past four decades. Predicting where and when the next arbovirus epidemic will occur is a challenge, but history suggests that arboviral black swan events (epidemics that are difficult to predict and that have an extreme effect) will continue to occur as urban growth and globalisation expand. We briefly review unexpected arbovirus epidemics that have occurred in the past 50 years, with emphasis on the American and Pacific regions, to illustrate their unpredictability, and to highlight the need for improved global preparedness, including laboratory-based surveillance, prevention, and control programmes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Pandemic arboviruses have emerged as a major global health problem in the past four decades. Predicting where and when the next arbovirus epidemic will occur is a challenge, but history suggests that arboviral black swan events (epidemics that are difficult to predict and that have an extreme effect) will continue to occur as urban growth and globalisation expand. We briefly review unexpected arbovirus epidemics that have occurred in the past 50 years, with emphasis on the American and Pacific regions, to illustrate their unpredictability, and to highlight the need for improved global preparedness, including laboratory-based surveillance, prevention, and control programmes. |
| 59. | Kucharski, A; Kama, M; Watson, C; Aubry, M; Funks, S; Henderson, A; Brady, O; Vanhomwegen, J; Manuguerra, J C; Lau, C; Edmunds, W; Aaskov, J; Nilles, E; Cao-Lormeau, V M; Hue, S; Hibberd, M: Using paired serology and surveillance data to quantify dengue transmission and control during a large outbreak in Fiji.. Dans: eLife, 7 , p. e34848, 2018. (type: Article de journal | Résumé | BibTeX) @article{5567, title = {Using paired serology and surveillance data to quantify dengue transmission and control during a large outbreak in Fiji.}, author = {A Kucharski and M Kama and C Watson and M Aubry and S Funks and A Henderson and O Brady and J Vanhomwegen and J C Manuguerra and C Lau and W Edmunds and J Aaskov and E Nilles and V M Cao-Lormeau and S Hue and M Hibberd}, year = {2018}, date = {2018-01-01}, journal = {eLife}, volume = {7}, pages = {e34848}, abstract = {Dengue is a major health burden, but it can be challenging to examine transmission and evaluate control measures because outbreaks depend on multiple factors, including human population structure, prior immunity and climate. We combined population-representative paired sera collected before and after the 2013/14 dengue-3 outbreak in Fiji with surveillance data to determine how such factors influence transmission and control in island settings. Our results suggested the 10-19 year-old age group had the highest risk of infection, but we did not find strong evidence that other demographic or environmental risk factors were linked to seroconversion. A mathematical model jointly fitted to surveillance and serological data suggested that herd immunity and seasonally varying transmission could not explain observed dynamics. However, the model showed evidence of an additional reduction in transmission coinciding with a vector clean-up campaign, which may have contributed to the decline in cases in the later stages of the outbreak.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dengue is a major health burden, but it can be challenging to examine transmission and evaluate control measures because outbreaks depend on multiple factors, including human population structure, prior immunity and climate. We combined population-representative paired sera collected before and after the 2013/14 dengue-3 outbreak in Fiji with surveillance data to determine how such factors influence transmission and control in island settings. Our results suggested the 10-19 year-old age group had the highest risk of infection, but we did not find strong evidence that other demographic or environmental risk factors were linked to seroconversion. A mathematical model jointly fitted to surveillance and serological data suggested that herd immunity and seasonally varying transmission could not explain observed dynamics. However, the model showed evidence of an additional reduction in transmission coinciding with a vector clean-up campaign, which may have contributed to the decline in cases in the later stages of the outbreak. |
| 60. | Musso, D; Parola, P; Raoult, D: Yellow fever: the Pacific should be prepared.. Dans: Lancet, 392 , p. 2347, 2018. (type: Article de journal | BibTeX) @article{5577, title = {Yellow fever: the Pacific should be prepared.}, author = {D Musso and P Parola and D Raoult}, year = {2018}, date = {2018-01-01}, journal = {Lancet}, volume = {392}, pages = {2347}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 61. | Subissi, L; Dub, T; Besnard, M; Mariteragi-Helle, T; Nhan, T; Lutringer-Magnin, D; Barboza, P; Gurry, C; Nilles, E; Baud, D; Merianos, A; Musso, D; Glynn, J; Dupuis, G; Cao-Lormeau, V M; Giard, M; Mallet, H P: Zika Virus Infection during Pregnancy and Effects on Early Childhood Development, French Polynesia, 2013-2016.. Dans: Emerg Infect Dis, 24 , p. 1850-8, 2018. (type: Article de journal | Résumé | BibTeX) @article{5587, title = {Zika Virus Infection during Pregnancy and Effects on Early Childhood Development, French Polynesia, 2013-2016.}, author = {L Subissi and T Dub and M Besnard and T Mariteragi-Helle and T Nhan and D Lutringer-Magnin and P Barboza and C Gurry and E Nilles and D Baud and A Merianos and D Musso and J Glynn and G Dupuis and V M Cao-Lormeau and M Giard and H P Mallet}, year = {2018}, date = {2018-01-01}, journal = {Emerg Infect Dis}, volume = {24}, pages = {1850-8}, abstract = {Congenital Zika virus syndrome consists of a large spectrum of neurologic abnormalities seen in infants infected with Zika virus in utero. However, little is known about the effects of Zika virus intrauterine infection on the neurocognitive development of children born without birth defects. Using a case-control study design, we investigated the temporal association of a cluster of congenital defects with Zika virus infection. In a nested study, we also assessed the early childhood development of children recruited in the initial study as controls who were born without known birth defects,. We found evidence for an association of congenital defects with both maternal Zika virus seropositivity (time of infection unknown) and symptomatic Zika virus infection during pregnancy. Although the early childhood development assessment found no excess burden of developmental delay associated with maternal Zika virus infection, larger, longer-term studies are needed.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Congenital Zika virus syndrome consists of a large spectrum of neurologic abnormalities seen in infants infected with Zika virus in utero. However, little is known about the effects of Zika virus intrauterine infection on the neurocognitive development of children born without birth defects. Using a case-control study design, we investigated the temporal association of a cluster of congenital defects with Zika virus infection. In a nested study, we also assessed the early childhood development of children recruited in the initial study as controls who were born without known birth defects,. We found evidence for an association of congenital defects with both maternal Zika virus seropositivity (time of infection unknown) and symptomatic Zika virus infection during pregnancy. Although the early childhood development assessment found no excess burden of developmental delay associated with maternal Zika virus infection, larger, longer-term studies are needed. |
| 62. | Musso, D; Bossin, H C; Mallet, H P; Besnard, M; Broult, J; Baudouin, L; Levi, J E; Sabino, E C; Ghawche, F; Lanteri, M C; Baud, D: Zika Virus in French Polynesia, 2013-2014: anatomy of a completed outbreak (review paper).. Dans: Lancet Infect Dis, 18 , p. 2172-e182, 2018. (type: Article de journal | Résumé | BibTeX) @article{5579, title = {Zika Virus in French Polynesia, 2013-2014: anatomy of a completed outbreak (review paper).}, author = {D Musso and H C Bossin and H P Mallet and M Besnard and J Broult and L Baudouin and J E Levi and E C Sabino and F Ghawche and M C Lanteri and D Baud}, year = {2018}, date = {2018-01-01}, journal = {Lancet Infect Dis}, volume = {18}, pages = {2172-e182}, abstract = {The Zika virus crisis exemplified the risk associated with emerging pathogens and was a reminder that preparedness for the worst-case scenario, although challenging, is needed. Herein, we review all data reported during the unexpected emergence of Zika virus in French Polynesia in late 2013. We focus on the new findings reported during this outbreak, especially the first description of severe neurological complications in adults and the retrospective description of CNS malformations in neonates, the isolation of Zika virus in semen, the potential for blood-transfusion transmission, mother-to-child transmission, and the development of new diagnostic assays. We describe the effect of this outbreak on health systems, the implementation of vector-borne control strategies, and the line of communication used to alert the international community of the new risk associated with Zika virus. This outbreak highlighted the need for careful monitoring of all unexpected events that occur during an emergence, to implement surveillance and research programmes in parallel to management of cases, and to be prepared to the worst-case scenario.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Zika virus crisis exemplified the risk associated with emerging pathogens and was a reminder that preparedness for the worst-case scenario, although challenging, is needed. Herein, we review all data reported during the unexpected emergence of Zika virus in French Polynesia in late 2013. We focus on the new findings reported during this outbreak, especially the first description of severe neurological complications in adults and the retrospective description of CNS malformations in neonates, the isolation of Zika virus in semen, the potential for blood-transfusion transmission, mother-to-child transmission, and the development of new diagnostic assays. We describe the effect of this outbreak on health systems, the implementation of vector-borne control strategies, and the line of communication used to alert the international community of the new risk associated with Zika virus. This outbreak highlighted the need for careful monitoring of all unexpected events that occur during an emergence, to implement surveillance and research programmes in parallel to management of cases, and to be prepared to the worst-case scenario. |
| 63. | Masmejan, S; Baud, D; Musso, D; Panchaud, A: Zika virus, vaccines, and antiviral strategies.. Dans: Expert Rev Anti Infect Ther, 16 , p. 471-83, 2018. (type: Article de journal | Résumé | BibTeX) @article{5571, title = {Zika virus, vaccines, and antiviral strategies.}, author = {S Masmejan and D Baud and D Musso and A Panchaud}, year = {2018}, date = {2018-01-01}, journal = {Expert Rev Anti Infect Ther}, volume = {16}, pages = {471-83}, abstract = {Zika virus (ZIKV) recently emerged as a global public health emergency of international concern. ZIKV is responsible for severe neurological complications in adults and infection during pregnancy and can lead to congenital Zika syndrome. There is no licensed vaccine or drug to prevent or treat ZIKV infection. Areas covered: The aim of this article is to provide an overview and update of the progress of research on anti-ZIKV vaccine and medications until the end of 2017, with a special emphasis on drugs that can be used during pregnancy. Expert commentary: Development of new vaccines and drugs is challenging and several points particular to ZIKV infections augment this difficulty: (1) Cross-reactions between ZIKV and other flaviviruses, the impact of ZIKV vaccination on subsequent flavivirus infections, and vice-versa, is unknown, (2) Drugs against ZIKV should be safe in pregnant women, and (3) Evaluation of the efficacy of vaccine and drugs against ZIKV in clinical trials phase II-IV will be complicated due to the decline of ZIKV circulation.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Zika virus (ZIKV) recently emerged as a global public health emergency of international concern. ZIKV is responsible for severe neurological complications in adults and infection during pregnancy and can lead to congenital Zika syndrome. There is no licensed vaccine or drug to prevent or treat ZIKV infection. Areas covered: The aim of this article is to provide an overview and update of the progress of research on anti-ZIKV vaccine and medications until the end of 2017, with a special emphasis on drugs that can be used during pregnancy. Expert commentary: Development of new vaccines and drugs is challenging and several points particular to ZIKV infections augment this difficulty: (1) Cross-reactions between ZIKV and other flaviviruses, the impact of ZIKV vaccination on subsequent flavivirus infections, and vice-versa, is unknown, (2) Drugs against ZIKV should be safe in pregnant women, and (3) Evaluation of the efficacy of vaccine and drugs against ZIKV in clinical trials phase II-IV will be complicated due to the decline of ZIKV circulation. |
| 64. | Calvez, E; Mousson, L; Vazeille, M; O'Connor, O; Cao-Lormeau, V M; Mathieu-Daudé, F; Pocquet, N; Failloux, A B; Dupont-Rouzeyrol, M: Zika virus outbreak in the Pacific: Vector competence of regional vectors.. Dans: Plos Neg Trop Dis, 12 , p. e0006637, 2018. (type: Article de journal | Résumé | BibTeX) @article{5561, title = {Zika virus outbreak in the Pacific: Vector competence of regional vectors.}, author = {E Calvez and L Mousson and M Vazeille and O O'Connor and V M Cao-Lormeau and F Mathieu-Daudé and N Pocquet and A B Failloux and M Dupont-Rouzeyrol}, year = {2018}, date = {2018-01-01}, journal = {Plos Neg Trop Dis}, volume = {12}, pages = {e0006637}, abstract = {BackgroundIn 2013, Zika virus (ZIKV) emerged in French Polynesia and spread through the Pacific region between 2013 and 2017. Several potential Aedes mosquitoes may have contributed to the ZIKV transmission including Aedes aegypti, the main arbovirus vector in the region, and Aedes polynesiensis, vector of lymphatic filariasis and secondary vector of dengue virus. The aim of this study was to analyze the ability of these two Pacific vectors to transmit ZIKV at a regional scale, through the evaluation and comparison of the vector competence of wild Ae. aegypti and Ae. polynesiensis populations from different Pacific islands for a ZIKV strain which circulated in this region during the 2013-2017 outbreak. Methodology/Principal findings Field Ae. aegypti (three populations) and Ae. polynesiensis (two populations) from the Pacific region were collected for this study. Female mosquitoes were orally exposed to ZIKV (107 TCID50/mL) isolated in the region in 2014. At 6, 9, 14 and 21 days post-infection, mosquito bodies (thorax and abdomen), heads and saliva were analyzed to measure infection, dissemination, transmission rates and transmission efficiency, respectively. According to our results, ZIKV infection rates were heterogeneous between the Ae. aegypti populations, but the dissemination rates were moderate and more homogenous between these populations. For Ae. polynesiensis, infection rates were less heterogeneous between the two populations tested. The transmission rate and efficiency results revealed a low vector competence for ZIKV of the different Aedes vector populations under study. Conclusion/Significance Our results indicated a low ZIKV transmission by Ae. aegypti and Ae. polynesiensis tested from the Pacific region. These results were unexpected and suggest the importance of other factors especially the vector density, the mosquito lifespan or the large immunologically naive fraction of the population that may have contributed to the rapid spread of the ZIKV in the Pacific region during the 2013-2017 outbreak.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundIn 2013, Zika virus (ZIKV) emerged in French Polynesia and spread through the Pacific region between 2013 and 2017. Several potential Aedes mosquitoes may have contributed to the ZIKV transmission including Aedes aegypti, the main arbovirus vector in the region, and Aedes polynesiensis, vector of lymphatic filariasis and secondary vector of dengue virus. The aim of this study was to analyze the ability of these two Pacific vectors to transmit ZIKV at a regional scale, through the evaluation and comparison of the vector competence of wild Ae. aegypti and Ae. polynesiensis populations from different Pacific islands for a ZIKV strain which circulated in this region during the 2013-2017 outbreak. Methodology/Principal findings Field Ae. aegypti (three populations) and Ae. polynesiensis (two populations) from the Pacific region were collected for this study. Female mosquitoes were orally exposed to ZIKV (107 TCID50/mL) isolated in the region in 2014. At 6, 9, 14 and 21 days post-infection, mosquito bodies (thorax and abdomen), heads and saliva were analyzed to measure infection, dissemination, transmission rates and transmission efficiency, respectively. According to our results, ZIKV infection rates were heterogeneous between the Ae. aegypti populations, but the dissemination rates were moderate and more homogenous between these populations. For Ae. polynesiensis, infection rates were less heterogeneous between the two populations tested. The transmission rate and efficiency results revealed a low vector competence for ZIKV of the different Aedes vector populations under study. Conclusion/Significance Our results indicated a low ZIKV transmission by Ae. aegypti and Ae. polynesiensis tested from the Pacific region. These results were unexpected and suggest the importance of other factors especially the vector density, the mosquito lifespan or the large immunologically naive fraction of the population that may have contributed to the rapid spread of the ZIKV in the Pacific region during the 2013-2017 outbreak. |
| 65. | Richard, V; Paoaafaite, T; Cao-Lormeau, V M: Acquittal of Culex quinquefasciatus in transmitting Zika virus during the French Polynesian outbreak.. Dans: Acta Trop, 173 , p. 200-1, 2017. (type: Article de journal | BibTeX) @article{5621, title = {Acquittal of Culex quinquefasciatus in transmitting Zika virus during the French Polynesian outbreak.}, author = {V Richard and T Paoaafaite and V M Cao-Lormeau}, year = {2017}, date = {2017-01-01}, journal = {Acta Trop}, volume = {173}, pages = {200-1}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 66. | Meertens, L; Labeau, A; Dejarnac, O; Cipriani, S; Sinigaglia, L; Bonnet-Madin, L; Charpentier, Le T; Hafirassou, Lamine M; Zamborlini, A; Cao-Lormeau, V M; Coulpier, M; Missé, D; Jouvenet, N; Tabibiazar, R; Gressens, P; Schwartz, O; Amara, : Axl Mediates ZIKA Virus Entry in Human Glial Cells and Modulates Innate Immune Responses.. Dans: Cell Reports, 18 , p. 324-33, 2017. (type: Article de journal | Résumé | BibTeX) @article{5612, title = {Axl Mediates ZIKA Virus Entry in Human Glial Cells and Modulates Innate Immune Responses.}, author = {L Meertens and A Labeau and O Dejarnac and S Cipriani and L Sinigaglia and L Bonnet-Madin and T Le Charpentier and M Lamine Hafirassou and A Zamborlini and V M Cao-Lormeau and M Coulpier and D Missé and N Jouvenet and R Tabibiazar and P Gressens and O Schwartz and Amara}, year = {2017}, date = {2017-01-01}, journal = {Cell Reports}, volume = {18}, pages = {324-33}, abstract = {ZIKA virus (ZIKV) is an emerging pathogen responsible for neurological disorders and congenital microcephaly. However, the molecular basis for ZIKV neurotropism remains poorly understood. Here, we show that Axl is expressed in human microglia and astrocytes in the developing brain and thatitmediates ZIKV infection of glial cells. Axl-mediated ZIKV entry requires the Axl ligand Gas6, which bridges ZIKV particles to glial cells. Following binding, ZIKV is internalized through clathrin-mediated endocytosis and traffics to Rab5+ endosomes to establish productive infection. During entry, the ZIKV/Gas6 complex activates Axl kinase activity, which downmodulates interferon signaling and facilitates infection. ZIKV infection of human glial cells is inhibited by MYD1, an engineered Axl decoy receptor, and by the Axl kinase inhibitor R428. Our results highlight the dual role of Axl during ZIKV infection ofglial cells: promoting viral entry and modulating innate immune responses. Therefore, inhibiting Axl function may represent a potential target for future antiviral therapies.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ZIKA virus (ZIKV) is an emerging pathogen responsible for neurological disorders and congenital microcephaly. However, the molecular basis for ZIKV neurotropism remains poorly understood. Here, we show that Axl is expressed in human microglia and astrocytes in the developing brain and thatitmediates ZIKV infection of glial cells. Axl-mediated ZIKV entry requires the Axl ligand Gas6, which bridges ZIKV particles to glial cells. Following binding, ZIKV is internalized through clathrin-mediated endocytosis and traffics to Rab5+ endosomes to establish productive infection. During entry, the ZIKV/Gas6 complex activates Axl kinase activity, which downmodulates interferon signaling and facilitates infection. ZIKV infection of human glial cells is inhibited by MYD1, an engineered Axl decoy receptor, and by the Axl kinase inhibitor R428. Our results highlight the dual role of Axl during ZIKV infection ofglial cells: promoting viral entry and modulating innate immune responses. Therefore, inhibiting Axl function may represent a potential target for future antiviral therapies. |
| 67. | Laughhunn, A; Maria, Santa F; Broult, J; Lanteri, M C; Stassinopoulos, A; Musso, D; Aubry, M: Amustaline (S-303) Treatment Inactivates High Levels of Zika Virus in Red Blood Cell Components.. Dans: Transfusion, 57 , p. 779-89, 2017. (type: Article de journal | Résumé | BibTeX) @article{5609, title = {Amustaline (S-303) Treatment Inactivates High Levels of Zika Virus in Red Blood Cell Components.}, author = {A Laughhunn and F Santa Maria and J Broult and M C Lanteri and A Stassinopoulos and D Musso and M Aubry}, year = {2017}, date = {2017-01-01}, journal = {Transfusion}, volume = {57}, pages = {779-89}, abstract = {BackgroundThe potential for Zika virus (ZIKV) transfusion-transmission (TT) has been demonstrated in French Polynesia and Brazil. Pathogen inactivation (PI) of blood products is a proactive strategy to inactivate TT pathogens including arboviruses. Inactivation of West Nile, dengue, Zika, and chikungunya viruses was previously demonstrated by photochemical treatment with amotosalen and ultraviolet A (UVA) illumination. In this study, we evaluated ZIKV inactivation in red blood cell (RBC) components by a chemical approach that uses amustaline (S-303) and glutathione (GSH). Study design and methods RBC components were spiked with a high titer of ZIKV. Viral titers (infectivity) and ZIKV RNA loads (reverse transcription-polymerase chain reaction) were measured in spiked RBCs before and after S-303 and GSH treatment and confirmed using repetitive passages in cell culture. A mock-treated arm validated the approach by demonstrating stability of the virus (infectivity and RNA load) during the process. Results The mean ZIKV infectivity titer and RNA load in RBCs were 5.99 ± 0.2 log 50% tissue culture infectious dose (TCID50 )/mL and 7.75 ± 0.16 log genomic equivalents/mL before inactivation. No infectivity was detected immediately after S-303 and GSH treatment and after five serial passages in cell culture. Conclusion Complete ZIKV inactivation of more than 5.99 log TCID50 /mL in RBCs was achieved using S-303 and GSH at levels higher than those found in asymptomatic ZIKV-infected blood donors. Therefore, the S-303 and GSH PI system is promising for mitigating the risk of ZIKV TT.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundThe potential for Zika virus (ZIKV) transfusion-transmission (TT) has been demonstrated in French Polynesia and Brazil. Pathogen inactivation (PI) of blood products is a proactive strategy to inactivate TT pathogens including arboviruses. Inactivation of West Nile, dengue, Zika, and chikungunya viruses was previously demonstrated by photochemical treatment with amotosalen and ultraviolet A (UVA) illumination. In this study, we evaluated ZIKV inactivation in red blood cell (RBC) components by a chemical approach that uses amustaline (S-303) and glutathione (GSH). Study design and methods RBC components were spiked with a high titer of ZIKV. Viral titers (infectivity) and ZIKV RNA loads (reverse transcription-polymerase chain reaction) were measured in spiked RBCs before and after S-303 and GSH treatment and confirmed using repetitive passages in cell culture. A mock-treated arm validated the approach by demonstrating stability of the virus (infectivity and RNA load) during the process. Results The mean ZIKV infectivity titer and RNA load in RBCs were 5.99 ± 0.2 log 50% tissue culture infectious dose (TCID50 )/mL and 7.75 ± 0.16 log genomic equivalents/mL before inactivation. No infectivity was detected immediately after S-303 and GSH treatment and after five serial passages in cell culture. Conclusion Complete ZIKV inactivation of more than 5.99 log TCID50 /mL in RBCs was achieved using S-303 and GSH at levels higher than those found in asymptomatic ZIKV-infected blood donors. Therefore, the S-303 and GSH PI system is promising for mitigating the risk of ZIKV TT. |
| 68. | Baud, D; Gubler, D J; Schaub, B; Lanteri, M C; Musso, D: An update on Zika virus infection.. Dans: Lancet, 390 , p. 2099-2109, 2017. (type: Article de journal | Résumé | BibTeX) @article{5595, title = {An update on Zika virus infection.}, author = {D Baud and D J Gubler and B Schaub and M C Lanteri and D Musso}, year = {2017}, date = {2017-01-01}, journal = {Lancet}, volume = {390}, pages = {2099-2109}, abstract = {The epidemic history of Zika virus began in 2007, with its emergence in Yap Island in the western Pacific, followed in 2013-14 by a larger epidemic in French Polynesia, south Pacific, where the first severe complications and non-vector-borne transmission of the virus were reported. Zika virus emerged in Brazil in 2015 and was declared a national public health emergency after local researchers and physicians reported an increase in microcephaly cases. In 2016, WHO declared the recent cluster of microcephaly cases and other neurological disorders reported in Brazil a global public health emergency. Similar clusters of microcephaly cases were also observed retrospectively in French Polynesia in 2014. In 2015-16, Zika virus continued its spread to cause outbreaks in the Americas and the Pacific, and the first outbreaks were reported in continental USA, Africa, and southeast Asia. Non-vector-borne transmission was confirmed and Zika virus was established as a cause of severe neurological complications in fetuses, neonates, and adults. This Review focuses on important updates and gaps in the knowledge of Zika virus as of early 2017.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The epidemic history of Zika virus began in 2007, with its emergence in Yap Island in the western Pacific, followed in 2013-14 by a larger epidemic in French Polynesia, south Pacific, where the first severe complications and non-vector-borne transmission of the virus were reported. Zika virus emerged in Brazil in 2015 and was declared a national public health emergency after local researchers and physicians reported an increase in microcephaly cases. In 2016, WHO declared the recent cluster of microcephaly cases and other neurological disorders reported in Brazil a global public health emergency. Similar clusters of microcephaly cases were also observed retrospectively in French Polynesia in 2014. In 2015-16, Zika virus continued its spread to cause outbreaks in the Americas and the Pacific, and the first outbreaks were reported in continental USA, Africa, and southeast Asia. Non-vector-borne transmission was confirmed and Zika virus was established as a cause of severe neurological complications in fetuses, neonates, and adults. This Review focuses on important updates and gaps in the knowledge of Zika virus as of early 2017. |
| 69. | D'Mello, F; Braidy, N; Mareal, H; Guillemin, G; Rossi, F; Chinain, M; Laurent, D; Teo, C; Neilan, B A: Cytotoxic effects of environmental toxins on human glial cells.. Dans: Neurotox Res, 31 , p. 245-258, 2017. (type: Article de journal | Résumé | BibTeX) @article{5602, title = {Cytotoxic effects of environmental toxins on human glial cells.}, author = {F D'Mello and N Braidy and H Mareal and G Guillemin and F Rossi and M Chinain and D Laurent and C Teo and B A Neilan}, year = {2017}, date = {2017-01-01}, journal = {Neurotox Res}, volume = {31}, pages = {245-258}, abstract = {Toxins produced by cyanobacteria and dinoflagellates have increasingly become a public health concern due to their degenerative effects on mammalian tissue and cells. In particular, emerging evidence has called attention to the neurodegenerative effects of the cyanobacterial toxin ß-N-methylamino-L-alanine (BMAA). Other toxins such as the neurotoxins saxitoxin and ciguatoxin, as well as the hepatotoxic microcystin, have been previously shown to have a range of effects upon the nervous system. However, the capacity of these toxins to cause neurodegeneration in human cells has not, to our knowledge, been previously investigated. This study aimed to examine the cytotoxic effects of BMAA, microcystin-LR (MC-LR), saxitoxin (STX) and ciguatoxin (CTX-1B) on primary adult human astrocytes. We also demonstrated that a-lipoate attenuated MC-LR toxicity in primary astrocytes and characterised changes in gene expression which could potentially be caused by these toxins in primary astrocytes. Herein, we are the first to show that all of these toxins are capable of causing physiological changes consistent with neurodegeneration in glial cells, via oxidative stress and excitotoxicity, leading to a reduction in cell proliferation culminating in cell death. In addition, MC-LR toxicity was reduced significantly in astrocytes-treated a-lipoic acid. While there were no significant changes in gene expression, many of the probes that were altered were associated with neurodegenerative disease pathogenesis. Overall, this is important in advancing our current understanding of the mechanism of toxicity of MC-LR on human brain function in vitro, particularly in the context of neurodegeneration.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Toxins produced by cyanobacteria and dinoflagellates have increasingly become a public health concern due to their degenerative effects on mammalian tissue and cells. In particular, emerging evidence has called attention to the neurodegenerative effects of the cyanobacterial toxin ß-N-methylamino-L-alanine (BMAA). Other toxins such as the neurotoxins saxitoxin and ciguatoxin, as well as the hepatotoxic microcystin, have been previously shown to have a range of effects upon the nervous system. However, the capacity of these toxins to cause neurodegeneration in human cells has not, to our knowledge, been previously investigated. This study aimed to examine the cytotoxic effects of BMAA, microcystin-LR (MC-LR), saxitoxin (STX) and ciguatoxin (CTX-1B) on primary adult human astrocytes. We also demonstrated that a-lipoate attenuated MC-LR toxicity in primary astrocytes and characterised changes in gene expression which could potentially be caused by these toxins in primary astrocytes. Herein, we are the first to show that all of these toxins are capable of causing physiological changes consistent with neurodegeneration in glial cells, via oxidative stress and excitotoxicity, leading to a reduction in cell proliferation culminating in cell death. In addition, MC-LR toxicity was reduced significantly in astrocytes-treated a-lipoic acid. While there were no significant changes in gene expression, many of the probes that were altered were associated with neurodegenerative disease pathogenesis. Overall, this is important in advancing our current understanding of the mechanism of toxicity of MC-LR on human brain function in vitro, particularly in the context of neurodegeneration. |
| 70. | Chinain, M: Ciguatéra: connaissances d’antan, connaissances moderns.. Dans: Bulletin de la société des études océaniennes, 339 , p. 52-70, 2017. (type: Article de journal | BibTeX) @article{5601, title = {Ciguatéra: connaissances d’antan, connaissances moderns.}, author = {M Chinain}, year = {2017}, date = {2017-01-01}, journal = {Bulletin de la société des études océaniennes}, volume = {339}, pages = {52-70}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 71. | Musso, D; Richard, V; Teissier, A; Stone, M; Lanteri, M C; Latoni, G; Alsina, J; Reik, R; for the Busch, Recipient Epidemiology M P *; Group, Donor Evaluation Study REDS-III ZIKV Study: Detection of ZIKV RNA in semen of asymptomatic blood donors.. Dans: Clin Microbiol Infect, 23 , p. 1001.e1-1001.e3, 2017. (type: Article de journal | Résumé | BibTeX) @article{5617, title = {Detection of ZIKV RNA in semen of asymptomatic blood donors.}, author = {D Musso and V Richard and A Teissier and M Stone and M C Lanteri and G Latoni and J Alsina and R Reik and Recipient Epidemiology M P * for the Busch and Donor Evaluation Study REDS-III ZIKV Study Group}, year = {2017}, date = {2017-01-01}, journal = {Clin Microbiol Infect}, volume = {23}, pages = {1001.e1-1001.e3}, abstract = {ObjectivesZika virus (ZIKV) transmission through semen donation has never been reported but the risk is supported by the detection of ZIKV in semen and the demonstration of ZIKV sexual transmission. The potential impact of ZIKV on assisted reproductive procedures should be evaluated. Methods We tested longitudinally collected semen samples provided by asymptomatic blood donors who tested positive for ZIKV RNA in plasma during ZIKV outbreaks in Puerto Rico and Florida in 2016. Results Five of the 14 (35.7%) asymptomatic blood donors provided semen samples that tested positive for ZIKV RNA, with ZIKV RNA loads ranging from 8.03x103 to 2.55x106 copies/mL. Plasma collected at the same time as the semen tested negative for ZIKV RNA for most ZIKV RNA-positive semen collections; all corresponding plasma samples tested positive or equivocal for anti ZIKV IgG antibodies and all except one tested positive for ZIKV IgM antibodies. The rate of detection of ZIKV RNA in semen in asymptomatic donors is not significantly different from the rate previously reported for symptomatic patients. Conclusions Our results that show a high percentage of detection of ZIKV RNA in the semen of asymptomatic men confirm that ZIKV is a new threat for reproductive medicine and should have important implications for assisted reproductive technology. We recommend that semen donations from men at risk for ZIKV infection should be tested for ZIKV RNA, regardless of symptoms of ZIKV infection.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ObjectivesZika virus (ZIKV) transmission through semen donation has never been reported but the risk is supported by the detection of ZIKV in semen and the demonstration of ZIKV sexual transmission. The potential impact of ZIKV on assisted reproductive procedures should be evaluated. Methods We tested longitudinally collected semen samples provided by asymptomatic blood donors who tested positive for ZIKV RNA in plasma during ZIKV outbreaks in Puerto Rico and Florida in 2016. Results Five of the 14 (35.7%) asymptomatic blood donors provided semen samples that tested positive for ZIKV RNA, with ZIKV RNA loads ranging from 8.03x103 to 2.55x106 copies/mL. Plasma collected at the same time as the semen tested negative for ZIKV RNA for most ZIKV RNA-positive semen collections; all corresponding plasma samples tested positive or equivocal for anti ZIKV IgG antibodies and all except one tested positive for ZIKV IgM antibodies. The rate of detection of ZIKV RNA in semen in asymptomatic donors is not significantly different from the rate previously reported for symptomatic patients. Conclusions Our results that show a high percentage of detection of ZIKV RNA in the semen of asymptomatic men confirm that ZIKV is a new threat for reproductive medicine and should have important implications for assisted reproductive technology. We recommend that semen donations from men at risk for ZIKV infection should be tested for ZIKV RNA, regardless of symptoms of ZIKV infection. |
| 72. | Osman, D A; Phelippeau, M; Drancourt, M; Musso, D: Diversity of Mycobacterium tuberculosis lineages in French Polynesia.. Dans: J Microbiol Immunol Infect, 50 , p. 199-206, 2017. (type: Article de journal | Résumé | BibTeX) @article{5618, title = {Diversity of Mycobacterium tuberculosis lineages in French Polynesia.}, author = {D A Osman and M Phelippeau and M Drancourt and D Musso}, year = {2017}, date = {2017-01-01}, journal = {J Microbiol Immunol Infect}, volume = {50}, pages = {199-206}, abstract = {Background/purposeFrench Polynesia is an overseas territory located in the South Pacific. The incidence of tuberculosis in French Polynesia has been stable since 2000 with an average of 20 cases/y/100,000 inhabitants. Molecular epidemiology of Mycobacterium tuberculosis in French Polynesia is unknown because M. tuberculosis isolates have not been routinely genotyped. Methods From 2009 to 2012, 34 isolates collected from 32 French Polynesian patients were identified as M. tuberculosis by probe hybridization. These isolates were genotyped using spoligotyping and 24-loci mycobacterial interspersed repetitive units (MIRUs)-variable number of tandem repeat (VNTR). Spoligotype patterns obtained using commercial kits were compared with the online international database SITVIT. MIRU-VNTR genotyping was performed using an in-house protocol based on capillary electrophoresis sizing for 24-loci MIRU-VNTR genotyping. Results The results of the spoligotyping method revealed that 25 isolates grouped into six previously described spoligotypes [H1, H3, U likely (S), T1, Manu, and Beijing] and nine isolates grouped into six new spoligotypes. Comparison with the international database MIRU-VNTRplus distributed 30 isolates into five lineages (Haarlem, Latin American Mediterranean, S, X, and Beijing) and four as unassigned isolates. Conclusion Genotyping identified four phylogenetic lineages belonging to the modern Euro-American subgroup, one Beijing genotype responsible for worldwide pandemics, including remote islands in the South Pacific, and one Manu genotype of the ancestral lineage of M. tuberculosis.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background/purposeFrench Polynesia is an overseas territory located in the South Pacific. The incidence of tuberculosis in French Polynesia has been stable since 2000 with an average of 20 cases/y/100,000 inhabitants. Molecular epidemiology of Mycobacterium tuberculosis in French Polynesia is unknown because M. tuberculosis isolates have not been routinely genotyped. Methods From 2009 to 2012, 34 isolates collected from 32 French Polynesian patients were identified as M. tuberculosis by probe hybridization. These isolates were genotyped using spoligotyping and 24-loci mycobacterial interspersed repetitive units (MIRUs)-variable number of tandem repeat (VNTR). Spoligotype patterns obtained using commercial kits were compared with the online international database SITVIT. MIRU-VNTR genotyping was performed using an in-house protocol based on capillary electrophoresis sizing for 24-loci MIRU-VNTR genotyping. Results The results of the spoligotyping method revealed that 25 isolates grouped into six previously described spoligotypes [H1, H3, U likely (S), T1, Manu, and Beijing] and nine isolates grouped into six new spoligotypes. Comparison with the international database MIRU-VNTRplus distributed 30 isolates into five lineages (Haarlem, Latin American Mediterranean, S, X, and Beijing) and four as unassigned isolates. Conclusion Genotyping identified four phylogenetic lineages belonging to the modern Euro-American subgroup, one Beijing genotype responsible for worldwide pandemics, including remote islands in the South Pacific, and one Manu genotype of the ancestral lineage of M. tuberculosis. |
| 73. | Calvez, E; Guillaumot, L; Girault, D; Richard, V; O'Connor, O; Paoaafaite, T; Teurlai, M; Pocquet, N; Cao-Lormeau, V M; Dupont-Rouzeyrol, M: Dengue-1 virus and vector competence of Aedes aegypti (Diptera: Culicidae) populations from New Caledonia.. Dans: Parasite Vectors, 10 , p. 381, 2017. (type: Article de journal | BibTeX) @article{5600, title = {Dengue-1 virus and vector competence of Aedes aegypti (Diptera: Culicidae) populations from New Caledonia.}, author = {E Calvez and L Guillaumot and D Girault and V Richard and O O'Connor and T Paoaafaite and M Teurlai and N Pocquet and V M Cao-Lormeau and M Dupont-Rouzeyrol}, year = {2017}, date = {2017-01-01}, journal = {Parasite Vectors}, volume = {10}, pages = {381}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 74. | Musso, D; Lanteri, M C: Emergence of Zika virus: where does it come from and where is it going to?. Dans: Lancet Infect Dis, 17 , p. 255, 2017. (type: Article de journal | BibTeX) @article{5614, title = {Emergence of Zika virus: where does it come from and where is it going to?}, author = {D Musso and M C Lanteri}, year = {2017}, date = {2017-01-01}, journal = {Lancet Infect Dis}, volume = {17}, pages = {255}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 75. | Lequime, S; Richard, V; Cao-Lormeau, V M; Lambrechts, L: Full-genome dengue virus sequencing in mosquito saliva shows lack of convergent positive selection during transmission by Aedes aegypti.. Dans: Virus Evol, 3 , p. vex031, 2017. (type: Article de journal | BibTeX) @article{5610, title = {Full-genome dengue virus sequencing in mosquito saliva shows lack of convergent positive selection during transmission by Aedes aegypti.}, author = {S Lequime and V Richard and V M Cao-Lormeau and L Lambrechts}, year = {2017}, date = {2017-01-01}, journal = {Virus Evol}, volume = {3}, pages = {vex031}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 76. | Gubler, D J; Vasilakis, N; Musso, D: History and emergence of Zika virus.. Dans: J Infect Dis, 216 , p. S860-7, 2017. (type: Article de journal | BibTeX) @article{5607, title = {History and emergence of Zika virus.}, author = {D J Gubler and N Vasilakis and D Musso}, year = {2017}, date = {2017-01-01}, journal = {J Infect Dis}, volume = {216}, pages = {S860-7}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 77. | Berdalet, E; Tester, P A; Chinain, M; Fraga, S; Lemee, R; Litaker, R W; Penna, A; Usup, G; Vila, M; Zingone, A: Harmful algal blooms in benthic systems: Recent progress and future research.. Dans: Oceanography, 30 , p. 36-45, 2017. (type: Article de journal | Résumé | BibTeX) @article{5597, title = {Harmful algal blooms in benthic systems: Recent progress and future research.}, author = {E Berdalet and P A Tester and M Chinain and S Fraga and R Lemee and R W Litaker and A Penna and G Usup and M Vila and A Zingone}, year = {2017}, date = {2017-01-01}, journal = {Oceanography}, volume = {30}, pages = {36-45}, abstract = {Shallow, well-illuminated coastal waters from tropical to temperate latitudes are attractive environments for humans. Beaches and coral reefs have provided lodging and food to coastal communities for centuries. Unfortunately, tropical regions traditionally have been threatened by outbreaks of the toxic benthic dinoflagellate Gambierdiscus, which is associated with ciguatera fish poisoning. The ciguatoxins produced by Gambierdiscus bioaccumulate in reef fishes and are responsible for the most common algal toxin-related illnesses, globally affecting the greatest number of victims and often with significant long-term health effects. Recently, Gambierdiscus has been documented in subtropical and temperate latitudes. Blooms of another benthic and toxic dinoflagellate, Ostreopsis, have become more frequent and intense, especially in temperate waters. Ostreopsis produces palytoxins and analogues, and some outbreaks have been associated with massive benthic faunal damage and respiratory irritations in humans exposed to aerosols. The increased frequency of harmful events and the biogeographic extension of benthic microalgae incentivized the launch of the Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) Core Research Project on “Benthic Harmful Algal Blooms” in 2010. This article summarizes the main scientific advances and gaps in related knowledge as well as advances the project has made toward managing and mitigating the impacts of benthic HABs on human illnesses and marine resource losses.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Shallow, well-illuminated coastal waters from tropical to temperate latitudes are attractive environments for humans. Beaches and coral reefs have provided lodging and food to coastal communities for centuries. Unfortunately, tropical regions traditionally have been threatened by outbreaks of the toxic benthic dinoflagellate Gambierdiscus, which is associated with ciguatera fish poisoning. The ciguatoxins produced by Gambierdiscus bioaccumulate in reef fishes and are responsible for the most common algal toxin-related illnesses, globally affecting the greatest number of victims and often with significant long-term health effects. Recently, Gambierdiscus has been documented in subtropical and temperate latitudes. Blooms of another benthic and toxic dinoflagellate, Ostreopsis, have become more frequent and intense, especially in temperate waters. Ostreopsis produces palytoxins and analogues, and some outbreaks have been associated with massive benthic faunal damage and respiratory irritations in humans exposed to aerosols. The increased frequency of harmful events and the biogeographic extension of benthic microalgae incentivized the launch of the Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) Core Research Project on “Benthic Harmful Algal Blooms” in 2010. This article summarizes the main scientific advances and gaps in related knowledge as well as advances the project has made toward managing and mitigating the impacts of benthic HABs on human illnesses and marine resource losses. |
| 78. | Aubry, M; Teissier, Y; Mapotoeke, M; Teissier, A; Giard, M; Musso, D; Cao-Lormeau, V M: High risk of dengue type 2 outbreak in French Polynesia, 2017.. Dans: Eurosurveillance, 22 , p. pii: 30505, 2017. (type: Article de journal | Résumé | BibTeX) @article{5594, title = {High risk of dengue type 2 outbreak in French Polynesia, 2017.}, author = {M Aubry and Y Teissier and M Mapotoeke and A Teissier and M Giard and D Musso and V M Cao-Lormeau}, year = {2017}, date = {2017-01-01}, journal = {Eurosurveillance}, volume = {22}, pages = {pii: 30505}, abstract = {In French Polynesia, the four serotypes of dengue virus (DENV-1 to -4) have caused 14 epidemics since the mid-1940s. From the end of 2016, an increasing number of Pacific Island Countries and Territories have reported DENV-2 outbreaks and in February 2017, DENV-2 infection was detected in French Polynesia in three travellers from Vanuatu. As DENV-2 has not been circulating in French Polynesia since December 2000, there is high risk for an outbreak to occur.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In French Polynesia, the four serotypes of dengue virus (DENV-1 to -4) have caused 14 epidemics since the mid-1940s. From the end of 2016, an increasing number of Pacific Island Countries and Territories have reported DENV-2 outbreaks and in February 2017, DENV-2 infection was detected in French Polynesia in three travellers from Vanuatu. As DENV-2 has not been circulating in French Polynesia since December 2000, there is high risk for an outbreak to occur. |
| 79. | Maria, Santa F; Lanteri, M C; Aubry, M; Musso, D; Stassinopoulos, A: Inactivation of Zika virus in platelet components using amotosalen and ultraviolet A illumination.. Dans: Transfusion, 57 , p. 2016-25, 2017. (type: Article de journal | Résumé | BibTeX) @article{5622, title = {Inactivation of Zika virus in platelet components using amotosalen and ultraviolet A illumination.}, author = {F Santa Maria and M C Lanteri and M Aubry and D Musso and A Stassinopoulos}, year = {2017}, date = {2017-01-01}, journal = {Transfusion}, volume = {57}, pages = {2016-25}, abstract = {BackgroundConcerned over the risk of Zika virus (ZIKV) transfusion transmission, public health agencies recommended the implementation of mitigation strategies for its prevention. Those strategies included the use of pathogen inactivation for the treatment of plasma and platelets. The efficacy of amotosalen/ultraviolet A to inactivate ZIKV in plasma had been previously demonstrated, and the efficacy of inactivation in platelets with the same technology was assumed. These studies quantify ZIKV inactivation in platelet components using amotosalen/ultraviolet A. Study Design And Methods Platelet components were spiked with ZIKV, and ZIKV infectious titers and RNA loads were measured by cell culture-based assays and real-time polymerase chain reaction in spiked platelet components before and after photochemical treatment using amotosalen/ultraviolet A. Results The mean ZIKV infectivity titers and RNA loads in platelet components before inactivation were either 4.9 log10 plaque forming units per milliliter, or 4.4 log10 50% tissue culture infective dose per milliliter and 7.5 log10 genome equivalents per milliliter, respectively. No infectivity was detected immediately after amotosalen/ultraviolet A treatment. No replicative virus remained after treatment, as demonstrated by multiple passages on Vero cell cultures; and ZIKV RNA was not detected from the first passage after inactivation. Additional experiments in this study demonstrated efficient inactivation to the limit of detection in platelets manufactured in 65% platelet additive solution, 35% plasma, or 100% plasma. Conclusion As previously demonstrated for plasma, robust levels of ZIKV inactivation were achieved in platelet components. With inactivation of higher levels of ZIKV than those reported in asymptomatic, RNA-reactive blood donors, the pathogen-inactivation system using amotosalen/ultraviolet A offers the potential to mitigate the risk of ZIKV transmission by plasma and platelet transfusion.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundConcerned over the risk of Zika virus (ZIKV) transfusion transmission, public health agencies recommended the implementation of mitigation strategies for its prevention. Those strategies included the use of pathogen inactivation for the treatment of plasma and platelets. The efficacy of amotosalen/ultraviolet A to inactivate ZIKV in plasma had been previously demonstrated, and the efficacy of inactivation in platelets with the same technology was assumed. These studies quantify ZIKV inactivation in platelet components using amotosalen/ultraviolet A. Study Design And Methods Platelet components were spiked with ZIKV, and ZIKV infectious titers and RNA loads were measured by cell culture-based assays and real-time polymerase chain reaction in spiked platelet components before and after photochemical treatment using amotosalen/ultraviolet A. Results The mean ZIKV infectivity titers and RNA loads in platelet components before inactivation were either 4.9 log10 plaque forming units per milliliter, or 4.4 log10 50% tissue culture infective dose per milliliter and 7.5 log10 genome equivalents per milliliter, respectively. No infectivity was detected immediately after amotosalen/ultraviolet A treatment. No replicative virus remained after treatment, as demonstrated by multiple passages on Vero cell cultures; and ZIKV RNA was not detected from the first passage after inactivation. Additional experiments in this study demonstrated efficient inactivation to the limit of detection in platelets manufactured in 65% platelet additive solution, 35% plasma, or 100% plasma. Conclusion As previously demonstrated for plasma, robust levels of ZIKV inactivation were achieved in platelet components. With inactivation of higher levels of ZIKV than those reported in asymptomatic, RNA-reactive blood donors, the pathogen-inactivation system using amotosalen/ultraviolet A offers the potential to mitigate the risk of ZIKV transmission by plasma and platelet transfusion. |
| 80. | Blumel, J; Musso, D; Teitz, S; Miyabayashi, T; Boller, K; Schnierle, B S; Baylis, S A: Inactivation and removal of Zika virus during manufacture of plasma derived medicinal products.. Dans: Transfusion, 57 , p. 790-6, 2017. (type: Article de journal | BibTeX) @article{5599, title = {Inactivation and removal of Zika virus during manufacture of plasma derived medicinal products.}, author = {J Blumel and D Musso and S Teitz and T Miyabayashi and K Boller and B S Schnierle and S A Baylis}, year = {2017}, date = {2017-01-01}, journal = {Transfusion}, volume = {57}, pages = {790-6}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 81. | Lyu, Y; Richlen, M L; Sehein, T R; Chinain, M; Adachi, M; Nishimura, T; Xu, Y; Parsons, M L; Smith, T B; Zheng, T; Anderson, D M: LSU rDNA based RFLP assays for the routine identification of Gambierdiscus species.. Dans: Harmful Algae, 66 , p. 20-8, 2017. (type: Article de journal | Résumé | BibTeX) @article{5611, title = {LSU rDNA based RFLP assays for the routine identification of Gambierdiscus species.}, author = {Y Lyu and M L Richlen and T R Sehein and M Chinain and M Adachi and T Nishimura and Y Xu and M L Parsons and T B Smith and T Zheng and D M Anderson}, year = {2017}, date = {2017-01-01}, journal = {Harmful Algae}, volume = {66}, pages = {20-8}, abstract = {The Gambierdiscus genus is a group of benthic dinoflagellates commonly associated with ciguatera fish poisoning (CFP), which is generally found in tropical or sub-tropical regions around the world. Morphologically similar species within the genus can vary in toxicity; however, species identifications are difficult or sometimes impossible using light microscopy. DNA sequencing of ribosomal RNA genes (rDNA) is thus often used to identify and describe Gambierdiscus species and ribotypes, but the expense and time can be prohibitive for routine culture screening and/or large-scale monitoring programs. This study describes a restriction fragment length polymorphism (RFLP) typing method based on analysis of the large subunit rDNA that can successfully identify at least nine of the described Gambierdiscus species and two Fukuyoa species. The software programs DNAMAN 6.0 and Restriction Enzyme Picker were used to identify a set of restriction enzymes (SpeI, HpyCH4IV, and TaqaI) capable of distinguishing most of the known Gambierdiscus species for which DNA sequences were available. This assay was tested using in silico analysis and cultured isolates, and species identifications of isolates assigned by RFLP typing were confirmed by DNA sequencing. To verify the assay and assess intra-specific heterogeneity in RFLP patterns, identifications of 63 Gambierdiscus isolates comprising ten Gambierdiscus species, one ribotype, and two Fukuyoa species were confirmed using RFLP typing, and this method was subsequently employed in the routine identification of isolates collected from the Caribbean Sea. The RFLP assay presented here reduces the time and cost associated with morphological identification via scanning electron microscopy and/or DNA sequencing, and provides a phylogenetically sensitive method for routine Gambierdiscus species assignment.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Gambierdiscus genus is a group of benthic dinoflagellates commonly associated with ciguatera fish poisoning (CFP), which is generally found in tropical or sub-tropical regions around the world. Morphologically similar species within the genus can vary in toxicity; however, species identifications are difficult or sometimes impossible using light microscopy. DNA sequencing of ribosomal RNA genes (rDNA) is thus often used to identify and describe Gambierdiscus species and ribotypes, but the expense and time can be prohibitive for routine culture screening and/or large-scale monitoring programs. This study describes a restriction fragment length polymorphism (RFLP) typing method based on analysis of the large subunit rDNA that can successfully identify at least nine of the described Gambierdiscus species and two Fukuyoa species. The software programs DNAMAN 6.0 and Restriction Enzyme Picker were used to identify a set of restriction enzymes (SpeI, HpyCH4IV, and TaqaI) capable of distinguishing most of the known Gambierdiscus species for which DNA sequences were available. This assay was tested using in silico analysis and cultured isolates, and species identifications of isolates assigned by RFLP typing were confirmed by DNA sequencing. To verify the assay and assess intra-specific heterogeneity in RFLP patterns, identifications of 63 Gambierdiscus isolates comprising ten Gambierdiscus species, one ribotype, and two Fukuyoa species were confirmed using RFLP typing, and this method was subsequently employed in the routine identification of isolates collected from the Caribbean Sea. The RFLP assay presented here reduces the time and cost associated with morphological identification via scanning electron microscopy and/or DNA sequencing, and provides a phylogenetically sensitive method for routine Gambierdiscus species assignment. |
| 82. | Guernier, V; Richard, V; Nhan, T; Rouault, E; Teissier, A; Musso, D: Leptospira diversity in animals and humans in Tahiti, French Polynesia.. Dans: Plos Neg Trop Dis, 11 , p. e0005676, 2017. (type: Article de journal | Résumé | BibTeX) @article{5606, title = {Leptospira diversity in animals and humans in Tahiti, French Polynesia.}, author = {V Guernier and V Richard and T Nhan and E Rouault and A Teissier and D Musso}, year = {2017}, date = {2017-01-01}, journal = {Plos Neg Trop Dis}, volume = {11}, pages = {e0005676}, abstract = {BackgroundLeptospirosis is a highly endemic bacterial zoonosis in French Polynesia (FP). Nevertheless, data on the epidemiology of leptospirosis in FP are scarce. We conducted molecular studies on Leptospira isolated from humans and the potential main animal reservoirs in order to identify the most likely sources for human infection. Methodology/Principal Findings Wild rats (n = 113), farm pigs (n = 181) and domestic dogs (n = 4) were screened for Leptospira infection in Tahiti, the most populated island in FP. Positive samples were genotyped and compared to Leptospira isolated from human cases throughout FP (n = 51), using secY, 16S and LipL32 sequencing, and MLST analysis. Leptospira DNA was detected in 20.4% of rats and 26.5% of pigs. We identified two Leptospira species and three sequence types (STs) in animals and humans: Leptospira interrogans ST140 in pigs only and L. interrogans ST17 and Leptospira borgpetersenii ST149 in humans and rats. Overall, L. interrogans was the dominant species and grouped into four clades: one clade including a human case only, two clades including human cases and dogs, and one clade including human cases and rats. All except one pig sample showed a unique L. interrogans (secY) genotype distinct from those isolated from humans, rats and dogs. Moreover, LipL32 sequencing allowed the detection of an additional Leptospira genotype in pigs, clearly distinct from the previous ones. Conclusions/Significance Our data confirm rats as a major potential source for human leptospirosis in FP. By contrast to what was expected, farm pigs did not seem to be a major reservoir for the Leptospira genotypes identified in human patients. Thus, further investigations will be required to determine their significance in leptospirosis transmission in FP.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundLeptospirosis is a highly endemic bacterial zoonosis in French Polynesia (FP). Nevertheless, data on the epidemiology of leptospirosis in FP are scarce. We conducted molecular studies on Leptospira isolated from humans and the potential main animal reservoirs in order to identify the most likely sources for human infection. Methodology/Principal Findings Wild rats (n = 113), farm pigs (n = 181) and domestic dogs (n = 4) were screened for Leptospira infection in Tahiti, the most populated island in FP. Positive samples were genotyped and compared to Leptospira isolated from human cases throughout FP (n = 51), using secY, 16S and LipL32 sequencing, and MLST analysis. Leptospira DNA was detected in 20.4% of rats and 26.5% of pigs. We identified two Leptospira species and three sequence types (STs) in animals and humans: Leptospira interrogans ST140 in pigs only and L. interrogans ST17 and Leptospira borgpetersenii ST149 in humans and rats. Overall, L. interrogans was the dominant species and grouped into four clades: one clade including a human case only, two clades including human cases and dogs, and one clade including human cases and rats. All except one pig sample showed a unique L. interrogans (secY) genotype distinct from those isolated from humans, rats and dogs. Moreover, LipL32 sequencing allowed the detection of an additional Leptospira genotype in pigs, clearly distinct from the previous ones. Conclusions/Significance Our data confirm rats as a major potential source for human leptospirosis in FP. By contrast to what was expected, farm pigs did not seem to be a major reservoir for the Leptospira genotypes identified in human patients. Thus, further investigations will be required to determine their significance in leptospirosis transmission in FP. |
| 83. | Gerardin, P; Cao-Lormeau, V M; Tournebize, P; Cerny, T: Letter re: Acute Zika infection with concurrent onset of Guillain-BarrÉ syndrome.. Dans: Neurology, 88 , p. 1874, 2017. (type: Article de journal | BibTeX) @article{5605, title = {Letter re: Acute Zika infection with concurrent onset of Guillain-BarrÉ syndrome.}, author = {P Gerardin and V M Cao-Lormeau and P Tournebize and T Cerny}, year = {2017}, date = {2017-01-01}, journal = {Neurology}, volume = {88}, pages = {1874}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 84. | Phelippeau, M; Asmar, S; Osman, D A; Sassi, M; Robert, C; Musso, D; Drancourt, M: Mycobacterium massilipolynesiensis sp. nov., a rapidly-growing mycobacterium of medical interest related to Mycobacterium phlei.. Dans: Sci Rep, 7 , p. 40443, 2017. (type: Article de journal | Résumé | BibTeX) @article{5620, title = {Mycobacterium massilipolynesiensis sp. nov., a rapidly-growing mycobacterium of medical interest related to Mycobacterium phlei.}, author = {M Phelippeau and S Asmar and D A Osman and M Sassi and C Robert and D Musso and M Drancourt}, year = {2017}, date = {2017-01-01}, journal = {Sci Rep}, volume = {7}, pages = {40443}, abstract = {In French Polynesia, respiratory tract clinical isolate M26, displayed unusual phenotype and contradictory phylogenetic affiliations, suggesting a hitherto unidentified rapidly-growing Mycobacterium species. The phenotype of strain M26 was further characterized and its genome sequenced. Strain M26 genome consists in a 5,732,017-bp circular chromosome with a G?+?C% of 67.54%, comprising 5,500 protein-coding genes and 52 RNA genes (including two copies of the 16?S rRNA gene). One region coding for a putative prophage was also predicted. An intriguing characteristic of strain M26's genome is the large number of genes encoding polyketide synthases and nonribosomal peptide synthases. Phylogenomic analysis showed that strain M26's genome is closest to the Mycobacterium phlei genome with a 76.6% average nucleotide identity. Comparative genomics of 33 Mycobacterium genomes yielded 361 genes unique to M26 strain which functional annotation revealed 84.21% of unknown function and 3.88% encoding lipid transport and metabolism; while 48.87% of genes absent in M26 strain have unknown function, 9.5% are implicated in transcription and 19% are implicated in transport and metabolism. Strain M26's unique phenotypic and genomic characteristics indicate it is representative of a new species named "Mycobacterium massilipolynesiensis". Looking for mycobacteria in remote areas allows for the discovery of new Mycobacterium species.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In French Polynesia, respiratory tract clinical isolate M26, displayed unusual phenotype and contradictory phylogenetic affiliations, suggesting a hitherto unidentified rapidly-growing Mycobacterium species. The phenotype of strain M26 was further characterized and its genome sequenced. Strain M26 genome consists in a 5,732,017-bp circular chromosome with a G?+?C% of 67.54%, comprising 5,500 protein-coding genes and 52 RNA genes (including two copies of the 16?S rRNA gene). One region coding for a putative prophage was also predicted. An intriguing characteristic of strain M26's genome is the large number of genes encoding polyketide synthases and nonribosomal peptide synthases. Phylogenomic analysis showed that strain M26's genome is closest to the Mycobacterium phlei genome with a 76.6% average nucleotide identity. Comparative genomics of 33 Mycobacterium genomes yielded 361 genes unique to M26 strain which functional annotation revealed 84.21% of unknown function and 3.88% encoding lipid transport and metabolism; while 48.87% of genes absent in M26 strain have unknown function, 9.5% are implicated in transcription and 19% are implicated in transport and metabolism. Strain M26's unique phenotypic and genomic characteristics indicate it is representative of a new species named "Mycobacterium massilipolynesiensis". Looking for mycobacteria in remote areas allows for the discovery of new Mycobacterium species. |
| 85. | Chateau-Degat, M L; Beuter, A; Vauterin, G; Nguyen, N L; Chinain, M; Darius, H T; Legrand, A M; Chansin, R; Dewailly, E: Neurologic signs of ciguatera disease : evidence of their persistence.. Dans: Am J Trop Med Hyg, 77 , p. 1170-5, 2017. (type: Article de journal | Résumé | BibTeX) @article{5796, title = {Neurologic signs of ciguatera disease : evidence of their persistence.}, author = {M L Chateau-Degat and A Beuter and G Vauterin and N L Nguyen and M Chinain and H T Darius and A M Legrand and R Chansin and E Dewailly}, year = {2017}, date = {2017-01-01}, journal = {Am J Trop Med Hyg}, volume = {77}, pages = {1170-5}, abstract = {Ciguatoxins exert their effect on the voltage-sensitive sodium channel of the cellular membranes of all excitable tissues. This effect confers to ciguatera disease (CD) its neurologic hallmarks. A prospective study among French Polynesian adults over a two-month period was conducted to characterize and determine the persistence of neurologic symptoms of CD. We compared 47 patients with CD with 125 controls. In the acute phase of the disease, patients had mainly sensory disturbances as detected by an hypoesthesia on the palm of the hand and poorer sway performance compared with controls. Follow-up two months showed improvement of sway performance that eventually reaching control levels. However, for light-touch threshold, even if we observed a decrease threshold towards normal values, more than 50% of patients did not reach normal values 60 days after disease onset. Our results support the existence of neurologic impairments of CD and suggest their persistence for at least two months after onset.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ciguatoxins exert their effect on the voltage-sensitive sodium channel of the cellular membranes of all excitable tissues. This effect confers to ciguatera disease (CD) its neurologic hallmarks. A prospective study among French Polynesian adults over a two-month period was conducted to characterize and determine the persistence of neurologic symptoms of CD. We compared 47 patients with CD with 125 controls. In the acute phase of the disease, patients had mainly sensory disturbances as detected by an hypoesthesia on the palm of the hand and poorer sway performance compared with controls. Follow-up two months showed improvement of sway performance that eventually reaching control levels. However, for light-touch threshold, even if we observed a decrease threshold towards normal values, more than 50% of patients did not reach normal values 60 days after disease onset. Our results support the existence of neurologic impairments of CD and suggest their persistence for at least two months after onset. |
| 86. | Lau, C; Aubry, M; Musso, D; Teissier, A; Paulous, S; Despres, P; Lamballerie, De X; Pastorino, B; Cao-Lormeau, V M; Weinstein, P: New evidence for endemic circulation of Ross River Virus in the Pacific Islands and potential for emergence.. Dans: Int J Infect Dis, 57 , p. 73-6, 2017. (type: Article de journal | Résumé | BibTeX) @article{5608, title = {New evidence for endemic circulation of Ross River Virus in the Pacific Islands and potential for emergence.}, author = {C Lau and M Aubry and D Musso and A Teissier and S Paulous and P Despres and X De Lamballerie and B Pastorino and V M Cao-Lormeau and P Weinstein}, year = {2017}, date = {2017-01-01}, journal = {Int J Infect Dis}, volume = {57}, pages = {73-6}, abstract = {ObjectivesAn epidemic of Ross River virus (RRV) occurred in the South Pacific in 1979-1980, but RRV has not been thought to occur endemically outside Australia and Papua New Guinea. A seroprevalence study was conducted to determine whether RRV has circulated in American Samoa since 1980. Methods RRV ELISA IgG was performed on 200 serum samples collected in American Samoa in 2010; seroneutralization tests were performed on 60 representative samples. Results Of 196 available ELISA IgG results, 145 (74%, 95% confidence interval 67-80%) were seropositive. Of the 60 samples subjected to seroneutralization testing, none of the 15 ELISA IgG-negative and 16 of the 45 ELISA IgG-positive samples neutralized RRV. ELISA IgG seroprevalence was higher in persons born before/during the 1979-1980 RRV outbreak (78.3%), but was also high (63.0%) in people born after the outbreak who had lived their entire lives in American Samoa. Conclusions This study provides serological evidence that RRV circulation is likely to have occurred in American Samoa after 1980. Considering there are no marsupials in American Samoa, this finding implies that other species are capable of acting as reservoir hosts and indicates the potential for RRV to circulate in a much wider area than those currently recognized.}, keywords = {}, pubstate = {published}, tppubtype = {article} } ObjectivesAn epidemic of Ross River virus (RRV) occurred in the South Pacific in 1979-1980, but RRV has not been thought to occur endemically outside Australia and Papua New Guinea. A seroprevalence study was conducted to determine whether RRV has circulated in American Samoa since 1980. Methods RRV ELISA IgG was performed on 200 serum samples collected in American Samoa in 2010; seroneutralization tests were performed on 60 representative samples. Results Of 196 available ELISA IgG results, 145 (74%, 95% confidence interval 67-80%) were seropositive. Of the 60 samples subjected to seroneutralization testing, none of the 15 ELISA IgG-negative and 16 of the 45 ELISA IgG-positive samples neutralized RRV. ELISA IgG seroprevalence was higher in persons born before/during the 1979-1980 RRV outbreak (78.3%), but was also high (63.0%) in people born after the outbreak who had lived their entire lives in American Samoa. Conclusions This study provides serological evidence that RRV circulation is likely to have occurred in American Samoa after 1980. Considering there are no marsupials in American Samoa, this finding implies that other species are capable of acting as reservoir hosts and indicates the potential for RRV to circulate in a much wider area than those currently recognized. |
| 87. | Pagano, M; Rodier, M; Guillaumot, C; Thomas, Y; Henry, K; Andrefouet, S: Ocean-lagoon water and plankton exchanges in a semi-closed pearl farming atoll lagoon (Ahe, Tuamotu archipelago, French Polynesia).. Dans: Estuarine, Coastal and Shelf Science, 191 , p. 60-73, 2017. (type: Article de journal | Résumé | BibTeX) @article{5619, title = {Ocean-lagoon water and plankton exchanges in a semi-closed pearl farming atoll lagoon (Ahe, Tuamotu archipelago, French Polynesia).}, author = {M Pagano and M Rodier and C Guillaumot and Y Thomas and K Henry and S Andrefouet}, year = {2017}, date = {2017-01-01}, journal = {Estuarine, Coastal and Shelf Science}, volume = {191}, pages = {60-73}, abstract = {In atoll lagoons, plankton richness is highly dependent on water exchange with the ocean through the atoll rim. However, the dynamics of the physical and biological fluxes at the lagoon-ocean interface remain poorly characterized. Here, we studied the combined effects of lagoon-ocean water exchanges and local environmental conditions on the phyto- and zooplankton abundance and community structure across the atoll lagoon rim of Ahe (Tuamotu Archipelago, French Polynesia). Plankton and environmental variables were monitored in May 2013 (i) at several stations inside and outside the lagoon and (ii) during time-series corresponding to ebb-flood tidal cycles in the two types of channels connecting the lagoon to the ocean: at the passage (300 m long and about 11 m deep) and in hoa (i.e reef-flat less than 50 cm depth). Our results highlight tidally-driven selective plankton exchanges between the lagoon and external ocean. Phytoplankton (chlorophyll-a) and zooplankton biomass were respectively 4 times and 7 times higher in the lagoon than at stations outside the atoll lagoon. Copepoda was the dominant zooplanlcton group at the oceanic station (>75% abundance) whereas meroplankton (with bivalve larvae most common) was dominant at the lagoon stations (54%), in the passage (55-82%) and in hoa (>80%). These differences between sites suggest a loss of bivalve larvae through export to the ocean and retention and/or increased production of copepods in the lagoon. The daily export of bivalve larvae represents a low percentage of the lagoon stock, in agreement with previously published larval dispersal numerical models. The retention of copepods could constitute a significant input of nutrients and organic matter (through excretion, feces release, decomposition, and remineralization) into the lagoon.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In atoll lagoons, plankton richness is highly dependent on water exchange with the ocean through the atoll rim. However, the dynamics of the physical and biological fluxes at the lagoon-ocean interface remain poorly characterized. Here, we studied the combined effects of lagoon-ocean water exchanges and local environmental conditions on the phyto- and zooplankton abundance and community structure across the atoll lagoon rim of Ahe (Tuamotu Archipelago, French Polynesia). Plankton and environmental variables were monitored in May 2013 (i) at several stations inside and outside the lagoon and (ii) during time-series corresponding to ebb-flood tidal cycles in the two types of channels connecting the lagoon to the ocean: at the passage (300 m long and about 11 m deep) and in hoa (i.e reef-flat less than 50 cm depth). Our results highlight tidally-driven selective plankton exchanges between the lagoon and external ocean. Phytoplankton (chlorophyll-a) and zooplankton biomass were respectively 4 times and 7 times higher in the lagoon than at stations outside the atoll lagoon. Copepoda was the dominant zooplanlcton group at the oceanic station (>75% abundance) whereas meroplankton (with bivalve larvae most common) was dominant at the lagoon stations (54%), in the passage (55-82%) and in hoa (>80%). These differences between sites suggest a loss of bivalve larvae through export to the ocean and retention and/or increased production of copepods in the lagoon. The daily export of bivalve larvae represents a low percentage of the lagoon stock, in agreement with previously published larval dispersal numerical models. The retention of copepods could constitute a significant input of nutrients and organic matter (through excretion, feces release, decomposition, and remineralization) into the lagoon. |
| 88. | Musso, D; Broult, J; Bierlaire, D; Lanteri, M C; Aubry, M: Prevention of transfusion-transmitted Zika virus in French Polynesia, nucleic acid testing versus pathogen inactivation.. Dans: ISBT Science Series, 12 , p. 254-9, 2017. (type: Article de journal | Résumé | BibTeX) @article{5613, title = {Prevention of transfusion-transmitted Zika virus in French Polynesia, nucleic acid testing versus pathogen inactivation.}, author = {D Musso and J Broult and D Bierlaire and M C Lanteri and M Aubry}, year = {2017}, date = {2017-01-01}, journal = {ISBT Science Series}, volume = {12}, pages = {254-9}, abstract = {Background and Objectives French Polynesia (FP) experienced a large Zika virus (ZIKV) outbreak in 20132014. Flavivirus transfusion-transmission infections (TTIs) had been previously reported. We evaluated several mitigating strategies to prevent ZIKV TTIs in FP. Material and Methods Mitigation strategies consisted of pre/postdonation symptom reporting, donor deferral, importation of plasma units from non-endemic areas, quarantine of blood components, blood screening using a laboratory-developed nucleic acid testing (NAT) assay and platelet pathogen inactivation (PI) using the INTERCEPT Blood Systems. The pros and cons of NAT versus PI to prevent ZIKV TTIs were determined. Results Donor interview, deferral and component quarantine strategies were not sensitive enough to prevent subsequent detection of ZIKV RNA NAT-positive donations. A 12-week delay between the onset of outbreak and NAT implementation allowed for 30 blood products collected from 30 asymptomatic blood donors who retrospectively tested positive for ZIKV RNA by NAT to be transfused while platelet PI which was in routine use since 2010 in FP potentially prevented several cases of ZIKV TTIs. Conclusion Nucleic acid testing and PI were used in FP to prevent ZIKV TTIs. NAT was an effective measure once implemented but is not a proactive solution. PI is a proactive solution but with only systems approved for platelets and plasma. Based on the FP experience, there is an urgent need for approved whole blood and/or red blood cell PI systems and to develop multiplex NAT to maintain the availability of the blood supply in remote areas during arbovirus epidemics.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Background and Objectives French Polynesia (FP) experienced a large Zika virus (ZIKV) outbreak in 20132014. Flavivirus transfusion-transmission infections (TTIs) had been previously reported. We evaluated several mitigating strategies to prevent ZIKV TTIs in FP. Material and Methods Mitigation strategies consisted of pre/postdonation symptom reporting, donor deferral, importation of plasma units from non-endemic areas, quarantine of blood components, blood screening using a laboratory-developed nucleic acid testing (NAT) assay and platelet pathogen inactivation (PI) using the INTERCEPT Blood Systems. The pros and cons of NAT versus PI to prevent ZIKV TTIs were determined. Results Donor interview, deferral and component quarantine strategies were not sensitive enough to prevent subsequent detection of ZIKV RNA NAT-positive donations. A 12-week delay between the onset of outbreak and NAT implementation allowed for 30 blood products collected from 30 asymptomatic blood donors who retrospectively tested positive for ZIKV RNA by NAT to be transfused while platelet PI which was in routine use since 2010 in FP potentially prevented several cases of ZIKV TTIs. Conclusion Nucleic acid testing and PI were used in FP to prevent ZIKV TTIs. NAT was an effective measure once implemented but is not a proactive solution. PI is a proactive solution but with only systems approved for platelets and plasma. Based on the FP experience, there is an urgent need for approved whole blood and/or red blood cell PI systems and to develop multiplex NAT to maintain the availability of the blood supply in remote areas during arbovirus epidemics. |
| 89. | Aubry, M; Laughhunn, A; Maria, Santa F; Lanteri, M C; Stassinopoulos, A; Musso, D: Pathogen inactivation of Dengue virus in red blood cells using amustaline and glutathione.. Dans: Transfusion, 57 , p. 2888-96, 2017. (type: Article de journal | BibTeX) @article{5591, title = {Pathogen inactivation of Dengue virus in red blood cells using amustaline and glutathione.}, author = {M Aubry and A Laughhunn and F Santa Maria and M C Lanteri and A Stassinopoulos and D Musso}, year = {2017}, date = {2017-01-01}, journal = {Transfusion}, volume = {57}, pages = {2888-96}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 90. | Subissi, L; Daudens-Vaysse, E; Cassadou, S; Ledrans, M; Bompard, P; Gustave, J; Aubry, M; Cao-Lormeau, V M; Mallet, H P: Revising rates of asymptomatic Zika virus infections based on sentinel surveillance data from French Overseas Territories.. Dans: Int J Infect Dis, 65 , p. 116-8, 2017. (type: Article de journal | Résumé | BibTeX) @article{5624, title = {Revising rates of asymptomatic Zika virus infections based on sentinel surveillance data from French Overseas Territories.}, author = {L Subissi and E Daudens-Vaysse and S Cassadou and M Ledrans and P Bompard and J Gustave and M Aubry and V M Cao-Lormeau and H P Mallet}, year = {2017}, date = {2017-01-01}, journal = {Int J Infect Dis}, volume = {65}, pages = {116-8}, abstract = {French Polynesia and the French Territories of the Americas (FTAs) have experienced outbreaks of Zika virus (ZIKV) infection. These territories used similar sentinel syndromic surveillance to follow the epidemics. However, the surveillance system only takes into account consulting patients diagnosed with ZIKV disease, while non-consulting cases, as well as asymptomatic cases, are not taken into account. In the French territories under study, the ratio of consulting to non-consulting patients was found to likely be as low as 1/3 to 1/4, and rough estimates of the ZIKV asymptomatic infections indicated a lower rate than previously reported (i.e., not more than half).}, keywords = {}, pubstate = {published}, tppubtype = {article} } French Polynesia and the French Territories of the Americas (FTAs) have experienced outbreaks of Zika virus (ZIKV) infection. These territories used similar sentinel syndromic surveillance to follow the epidemics. However, the surveillance system only takes into account consulting patients diagnosed with ZIKV disease, while non-consulting cases, as well as asymptomatic cases, are not taken into account. In the French territories under study, the ratio of consulting to non-consulting patients was found to likely be as low as 1/3 to 1/4, and rough estimates of the ZIKV asymptomatic infections indicated a lower rate than previously reported (i.e., not more than half). |
| 91. | Stone, M; Lanteri, M C; Bakkour, S; Deng, X; Galel, S; Linnen, J; Munoz-Jordan, J; Lanciotti, R; Rios, M; Gallian, P; Musso, D; Leci, J; Bush, M: Relative analytical sensitivity of donor nucleic acid amplification technology screening and diagnostic real-time polymerase chain reaction assays for detection of Zika virus RNA.. Dans: Transfusion, 57 , p. 737-47, 2017. (type: Article de journal | Résumé | BibTeX) @article{5623, title = {Relative analytical sensitivity of donor nucleic acid amplification technology screening and diagnostic real-time polymerase chain reaction assays for detection of Zika virus RNA.}, author = {M Stone and M C Lanteri and S Bakkour and X Deng and S Galel and J Linnen and J Munoz-Jordan and R Lanciotti and M Rios and P Gallian and D Musso and J Leci and M Bush}, year = {2017}, date = {2017-01-01}, journal = {Transfusion}, volume = {57}, pages = {737-47}, abstract = {BackgroundZika virus (ZIKV) has spread rapidly in the Pacific and throughout the Americas and is associated with severe congenital and adult neurologic outcomes. Nucleic acid amplification technology (NAT) assays were developed for diagnostic applications and for blood donor screening on high-throughput NAT systems. We distributed blinded panels to compare the analytical performance of blood screening relative to diagnostic NAT assays. Study design and methods A 25-member, coded panel (11 half-log dilutions of a 2013 French Polynesia ZIKV isolate and 2015 Brazilian donor plasma implicated in transfusion transmission, and 3 negative controls) was sent to 11 laboratories that performed 17 assays with 2 to 12 replicates per panel member. Results were analyzed for the percentage reactivity at each dilution and by probit analysis to estimate the 50% and 95% limits of detection (LOD50 and LOD95 , respectively). Results Donor-screening NAT assays that process approximately 500 µL of plasma into amplification reactions were comparable in sensitivity (LOD50 and LOD95, 2.5 and 15-18 copies/mL) and were approximately 10-fold to 100-fold more sensitive than research laboratory-developed and diagnostic reverse transcriptase-polymerase chain reaction tests that process from 10 to 30 µL of plasma per amplification. Increasing sample input volume assayed with the Centers for Disease Control and Prevention reverse transcriptase-polymerase chain reaction assays increased the LODs by 10-fold to 30-fold. Conclusions Blood donor-screening ZIKV NAT assays demonstrate similar excellent sensitivities to assays currently used for screening for transfusion-transmitted viruses and are substantially more sensitive than most other laboratory-developed and diagnostic ZIKV reverse transcriptase-polymerase chain reaction assays. Enhancing sensitivities of laboratory-developed and diagnostic assays may be achievable by increasing sample input.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundZika virus (ZIKV) has spread rapidly in the Pacific and throughout the Americas and is associated with severe congenital and adult neurologic outcomes. Nucleic acid amplification technology (NAT) assays were developed for diagnostic applications and for blood donor screening on high-throughput NAT systems. We distributed blinded panels to compare the analytical performance of blood screening relative to diagnostic NAT assays. Study design and methods A 25-member, coded panel (11 half-log dilutions of a 2013 French Polynesia ZIKV isolate and 2015 Brazilian donor plasma implicated in transfusion transmission, and 3 negative controls) was sent to 11 laboratories that performed 17 assays with 2 to 12 replicates per panel member. Results were analyzed for the percentage reactivity at each dilution and by probit analysis to estimate the 50% and 95% limits of detection (LOD50 and LOD95 , respectively). Results Donor-screening NAT assays that process approximately 500 µL of plasma into amplification reactions were comparable in sensitivity (LOD50 and LOD95, 2.5 and 15-18 copies/mL) and were approximately 10-fold to 100-fold more sensitive than research laboratory-developed and diagnostic reverse transcriptase-polymerase chain reaction tests that process from 10 to 30 µL of plasma per amplification. Increasing sample input volume assayed with the Centers for Disease Control and Prevention reverse transcriptase-polymerase chain reaction assays increased the LODs by 10-fold to 30-fold. Conclusions Blood donor-screening ZIKV NAT assays demonstrate similar excellent sensitivities to assays currently used for screening for transfusion-transmitted viruses and are substantially more sensitive than most other laboratory-developed and diagnostic ZIKV reverse transcriptase-polymerase chain reaction assays. Enhancing sensitivities of laboratory-developed and diagnostic assays may be achievable by increasing sample input. |
| 92. | Aubry, M; Teissier, A; Huart, M; Merceron, S; Vanhomwegen, J; Roche, C; Vial, A L; Teururai, S; Sicard, S; Paulous, S; Despres, P; Manuguerra, J C; Mallet, H P; Musso, D; Deparis, X; Cao-Lormeau, V M: Ross River virus seroprevalence, French Polynesia, 2014-2015.. Dans: Emerg Infect Dis, 23 , p. 1751-3, 2017. (type: Article de journal | BibTeX) @article{5592, title = {Ross River virus seroprevalence, French Polynesia, 2014-2015.}, author = {M Aubry and A Teissier and M Huart and S Merceron and J Vanhomwegen and C Roche and A L Vial and S Teururai and S Sicard and S Paulous and P Despres and J C Manuguerra and H P Mallet and D Musso and X Deparis and V M Cao-Lormeau}, year = {2017}, date = {2017-01-01}, journal = {Emerg Infect Dis}, volume = {23}, pages = {1751-3}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 93. | Andronico, A; Dorléans, F; Fergé, J L; Salje, H; Ghawche, F; Signate, A; Daudens-Vaysse, E; Baudouin, L; Dub, T; Aubry, M; Cao-Lormeau, V M; Ledrans, M; Noel, H; Mallet, H P; Fontanet, A; Cabie, A; Cauchemez, S: Real-time assessment of healthcare requirements during the Zika virus epidemic in Martinique.. Dans: Am J Epidemiol, 186 , p. 1194-1203, 2017. (type: Article de journal | Résumé | BibTeX) @article{5590, title = {Real-time assessment of healthcare requirements during the Zika virus epidemic in Martinique.}, author = {A Andronico and F Dorléans and J L Fergé and H Salje and F Ghawche and A Signate and E Daudens-Vaysse and L Baudouin and T Dub and M Aubry and V M Cao-Lormeau and M Ledrans and H Noel and H P Mallet and A Fontanet and A Cabie and S Cauchemez}, year = {2017}, date = {2017-01-01}, journal = {Am J Epidemiol}, volume = {186}, pages = {1194-1203}, abstract = {The spread of Zika virus in the Americas has been associated with a surge in Guillain-Barré syndrome (GBS) cases. Given the severity of GBS, territories affected by Zika virus need to plan health-care resources to manage GBS patients. To inform such planning in Martinique, we analyzed Zika virus surveillance and GBS data from Martinique in real time with a modeling framework that captured dynamics of the Zika virus epidemic, the risk of GBS in Zika virus-infected persons, and the clinical management of GBS cases. We compared our estimates with those from the 2013-2014 Zika virus epidemic in French Polynesia. We were able to predict just a few weeks into the epidemic that, due to lower transmission potential and lower probability of developing GBS following infection in Martinique, the total number of GBS cases in Martinique would be substantially lower than suggested by simple extrapolations from French Polynesia. We correctly predicted that 8 intensive-care beds and 7 ventilators would be sufficient to treat GBS cases. This study showcased the contribution of modeling to inform local health-care planning during an outbreak. Timely studies that estimate the proportion of infected persons that seek care are needed to improve the predictive power of such approaches.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The spread of Zika virus in the Americas has been associated with a surge in Guillain-Barré syndrome (GBS) cases. Given the severity of GBS, territories affected by Zika virus need to plan health-care resources to manage GBS patients. To inform such planning in Martinique, we analyzed Zika virus surveillance and GBS data from Martinique in real time with a modeling framework that captured dynamics of the Zika virus epidemic, the risk of GBS in Zika virus-infected persons, and the clinical management of GBS cases. We compared our estimates with those from the 2013-2014 Zika virus epidemic in French Polynesia. We were able to predict just a few weeks into the epidemic that, due to lower transmission potential and lower probability of developing GBS following infection in Martinique, the total number of GBS cases in Martinique would be substantially lower than suggested by simple extrapolations from French Polynesia. We correctly predicted that 8 intensive-care beds and 7 ventilators would be sufficient to treat GBS cases. This study showcased the contribution of modeling to inform local health-care planning during an outbreak. Timely studies that estimate the proportion of infected persons that seek care are needed to improve the predictive power of such approaches. |
| 94. | Vouga, M; Musso, D; Shaub, B; Panchaud, A; Baud, D: Zika virus: are we going too far ?. Dans: Lancet, 389 , p. 151, 2017. (type: Article de journal | BibTeX) @article{5625, title = {Zika virus: are we going too far ?}, author = {M Vouga and D Musso and B Shaub and A Panchaud and D Baud}, year = {2017}, date = {2017-01-01}, journal = {Lancet}, volume = {389}, pages = {151}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 95. | Musso, D; Aubry, M; Broult, J; Stassinopoulos, A; Green, J: Zika virus: new emergencies, potential for severe complications, and prevention of transfusion-transmitted Zika fever in the context of arboviruses co-circulation.. Dans: Blood Transfusion, 15 , p. 272-3, 2017. (type: Article de journal | BibTeX) @article{5616, title = {Zika virus: new emergencies, potential for severe complications, and prevention of transfusion-transmitted Zika fever in the context of arboviruses co-circulation.}, author = {D Musso and M Aubry and J Broult and A Stassinopoulos and J Green}, year = {2017}, date = {2017-01-01}, journal = {Blood Transfusion}, volume = {15}, pages = {272-3}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 96. | Musso, D; Lanteri, M C: Zika virus in Singapore: unanswered questions.. Dans: Lancet Infect Dis, 17 , p. 782-3, 2017. (type: Article de journal | BibTeX) @article{5615, title = {Zika virus in Singapore: unanswered questions.}, author = {D Musso and M C Lanteri}, year = {2017}, date = {2017-01-01}, journal = {Lancet Infect Dis}, volume = {17}, pages = {782-3}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 97. | Dupont-Rouzeyrol, M; Diancourt, L; Calvez, E; Vandenbogaert, M; O'Connor, O; Teissier, A; Pol, M; Aubry, M; Faye, O; Tou, D; Cao-Lormeau, V M; Caro, V: Zika virus evolution in the edges of the Pacific Ocean.. Dans: Emerg Microbes Infect, 6 , p. e111, 2017. (type: Article de journal | BibTeX) @article{5603, title = {Zika virus evolution in the edges of the Pacific Ocean.}, author = {M Dupont-Rouzeyrol and L Diancourt and E Calvez and M Vandenbogaert and O O'Connor and A Teissier and M Pol and M Aubry and O Faye and D Tou and V M Cao-Lormeau and V Caro}, year = {2017}, date = {2017-01-01}, journal = {Emerg Microbes Infect}, volume = {6}, pages = {e111}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 98. | Gerardin, P; Cao-Lormeau, V M; Musso, D; Despres, P; Besnard, M: Zika rash and increased risk of congenital brain abnormalities.. Dans: Lancet, 389 , p. 151-2, 2017. (type: Article de journal | BibTeX) @article{5604, title = {Zika rash and increased risk of congenital brain abnormalities.}, author = {P Gerardin and V M Cao-Lormeau and D Musso and P Despres and M Besnard}, year = {2017}, date = {2017-01-01}, journal = {Lancet}, volume = {389}, pages = {151-2}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
| 99. | Bierlaire, D; Mauguin, S; Broult, J; Musso, D: Zika virus and blood transfusion, the experience of French Polynesia.. Dans: Transfusion, 57 , p. 729-33, 2017. (type: Article de journal | Résumé | BibTeX) @article{5598, title = {Zika virus and blood transfusion, the experience of French Polynesia.}, author = {D Bierlaire and S Mauguin and J Broult and D Musso}, year = {2017}, date = {2017-01-01}, journal = {Transfusion}, volume = {57}, pages = {729-33}, abstract = {BackgroundBetween October 2013 and March 2014, French Polynesia experienced the largest Zika virus (ZIKV) outbreak ever described before the emergence of ZIKV in the Americas in 2015. As arbovirus transfusion-transmitted (TT) infections have been previously reported, we hypothesized that transfusion of blood products could also transmit ZIKV. Study design and methods Mitigation strategies to prevent ZIKV-TT infections included nonspecific measures and the implementation of a laboratory developed ZIKV-specific nucleic acid testing (NAT) assay. Donor sera were tested in pools of 3 and constitutive sera of ZIKV-reactive pools were tested individually. Donor sera were tested prospectively and retrospectively. A posttransfusion follow-up of a patient transfused with ZIKV RNA-reactive blood products was implemented. Results NAT detected 42 blood donor sera as ZIKV RNA reactive of 1505 tested (2.8%). Thirty ZIKV RNA-reactive blood products collected before the implementation of NAT were transfused to 26 recipients. Posttransfusion investigations were conducted by the hemovigilance unit and data were available for 12 recipients. Symptomatic ZIKV-TT infections were not reported. Conclusion Predonation screening of blood donors, postdonation information, products discard, and quarantine of blood products were not effective enough to prevent transfusion of ZIKV RNA-reactive blood products. ZIKV NAT was an effective measure once implemented to prevent transfusion of ZIKV RNA-reactive blood products but it is difficult to evaluate the effectiveness of this measure to prevent ZIKV-TT infection, which is a rare event.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BackgroundBetween October 2013 and March 2014, French Polynesia experienced the largest Zika virus (ZIKV) outbreak ever described before the emergence of ZIKV in the Americas in 2015. As arbovirus transfusion-transmitted (TT) infections have been previously reported, we hypothesized that transfusion of blood products could also transmit ZIKV. Study design and methods Mitigation strategies to prevent ZIKV-TT infections included nonspecific measures and the implementation of a laboratory developed ZIKV-specific nucleic acid testing (NAT) assay. Donor sera were tested in pools of 3 and constitutive sera of ZIKV-reactive pools were tested individually. Donor sera were tested prospectively and retrospectively. A posttransfusion follow-up of a patient transfused with ZIKV RNA-reactive blood products was implemented. Results NAT detected 42 blood donor sera as ZIKV RNA reactive of 1505 tested (2.8%). Thirty ZIKV RNA-reactive blood products collected before the implementation of NAT were transfused to 26 recipients. Posttransfusion investigations were conducted by the hemovigilance unit and data were available for 12 recipients. Symptomatic ZIKV-TT infections were not reported. Conclusion Predonation screening of blood donors, postdonation information, products discard, and quarantine of blood products were not effective enough to prevent transfusion of ZIKV RNA-reactive blood products. ZIKV NAT was an effective measure once implemented to prevent transfusion of ZIKV RNA-reactive blood products but it is difficult to evaluate the effectiveness of this measure to prevent ZIKV-TT infection, which is a rare event. |
| 100. | Baud, D; Musso, D; Vouga, M; Alves, M P; ulliemoz, N: Zika virus: a new threat to human reproduction.. Dans: American Journal of Reproductive Immunology, 77 , p. doi:0.1111/aji.12614., 2017. (type: Article de journal | Résumé | BibTeX) @article{5596, title = {Zika virus: a new threat to human reproduction.}, author = {D Baud and D Musso and M Vouga and M P Alves and N ulliemoz}, year = {2017}, date = {2017-01-01}, journal = {American Journal of Reproductive Immunology}, volume = {77}, pages = {doi:0.1111/aji.12614.}, abstract = {Zika virus (ZIKV) was first isolated in 1947 in a rhesus monkey from the Zika forest of Uganda. Until 2007, only 14 human cases were reported. The first large human outbreak occurred in 2007 (Yap Island, Federated States of Micronesia, Pacific) followed by French Polynesia in 2013 and Brazil in 2015. The virus is mainly transmitted through Aedes mosquito bites, but sexual and post-transfusion transmissions have been reported. Symptoms include low-grade fever, maculopapular rash, conjunctivitis, myalgia, arthralgia, and asthenia. During the recent outbreaks in French Polynesia and Brazil, ZIKV infection has been associated with two major complications: microcephaly and Guillain-Barré syndrome. Since fetal infection includes other birth defects, congenital Zika syndrome has been used to define in utero infection. The majority of sexual transmission occurred from a symptomatic male to a female, but female-to-male and male-to-male transmission have been reported. Asymptomatic male-to-female transmission has also been described. Importantly, ZIKV RNA can persist at least 6 months in semen. The male urogenital tract may therefore act as a reservoir for the virus. ZIKV RNA was detected in a cervical swab of a patient 3 days after presenting the classic symptoms suggesting a potential tropism for the female genital tract. Long-lasting presence of ZIKV RNA might not indicate that the individual is infectious but makes recommendation for couples potentially exposed to the virus and willing to conceive difficult. It will also be important to determine whether genital ZIKV infection might have a deleterious effect on male and female fertility.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Zika virus (ZIKV) was first isolated in 1947 in a rhesus monkey from the Zika forest of Uganda. Until 2007, only 14 human cases were reported. The first large human outbreak occurred in 2007 (Yap Island, Federated States of Micronesia, Pacific) followed by French Polynesia in 2013 and Brazil in 2015. The virus is mainly transmitted through Aedes mosquito bites, but sexual and post-transfusion transmissions have been reported. Symptoms include low-grade fever, maculopapular rash, conjunctivitis, myalgia, arthralgia, and asthenia. During the recent outbreaks in French Polynesia and Brazil, ZIKV infection has been associated with two major complications: microcephaly and Guillain-Barré syndrome. Since fetal infection includes other birth defects, congenital Zika syndrome has been used to define in utero infection. The majority of sexual transmission occurred from a symptomatic male to a female, but female-to-male and male-to-male transmission have been reported. Asymptomatic male-to-female transmission has also been described. Importantly, ZIKV RNA can persist at least 6 months in semen. The male urogenital tract may therefore act as a reservoir for the virus. ZIKV RNA was detected in a cervical swab of a patient 3 days after presenting the classic symptoms suggesting a potential tropism for the female genital tract. Long-lasting presence of ZIKV RNA might not indicate that the individual is infectious but makes recommendation for couples potentially exposed to the virus and willing to conceive difficult. It will also be important to determine whether genital ZIKV infection might have a deleterious effect on male and female fertility. |
