@article { 2478, title = {Use of Centrifugal Filter Devices to Concentrate Dengue Virus in Mosquito per os Infection Experiments.}, journal = {PLoS ONE}, volume = {10}, year = {2015}, month = {2015}, pages = {e0138161}, abstract = {Dengue virus (DENV) is an arbovirus transmitted to humans by the bite of infected Aedes mosquitoes. Experimental per os infection of mosquitoes with DENV is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. We report here the convenient use of centrifugal filter devices to quickly concentrate DENV particles in cell-culture supernatants. The median viral titer in concentrated-supernatants was 8.50 log10 TCID50/mL. By using these DENV concentrated-supernatants to prepare infectious blood-meals in Aedes aegypti per os infection experiments, we obtained a mean mosquito-infection rate of 94%. We also evaluated the use of centrifugal filter devices to recover DENV particles from non-infectious blood-meals presented to infected mosquitoes through a feeding membrane to collect their saliva.}, author = {Richard, V. and Viallon, J. and Cao-Lormeau, V. M.} }